Perivascular support of human hematopoietic stem/progenitor cells

Corselli, Mirko; Chin, Chee Jia; Parekh, Chintan; Sahaghian, Arineh; Wang, Wenyuan; Ge, Shundi; Evseenko, Denis; Wang, Xiaoyan; Montelatici, Elisa; Lazzari, Lorenza; Crooks, Gay M.; Péault, Bruno

doi:10.1182/blood-2012-08-451864

Cited by 168 publications

(165 citation statements)

References 53 publications

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“…Natural forerunners of culture‐defined MSCs have been identified as pericytes 19 and ASC 20. We assessed the expression of a number of genes including Mospd1 by RNA sequencing in freshly sorted pericytes (CD146+ CD45− CD31− CD34−) and ASCs (CD34+CD146−CD45−CD31−) and then also in these cells following culture for three passages.…”

Section: Resultsmentioning

confidence: 99%

A Role for MOSPD1 in Mesenchymal Stem Cell Proliferation and Differentiation

et al. 2015

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Mesenchymal stem cells (MSCs) isolated from many tissues including bone marrow and fat can be expanded in vitro and can differentiate into a range of different cell types such as bone, cartilage, and adipocytes. MSCs can also exhibit immunoregulatory properties when transplanted but, although a number of clinical trials using MSCs are in progress, the molecular mechanisms that control their production, proliferation, and differentiation are poorly understood. We identify MOSPD1 as a new player in this process. We generated MOSPD1‐null embryonic stem cells (ESCs) and demonstrate that they are deficient in their ability to differentiate into a number of cell lineages including osteoblasts, adipocytes, and hematopoietic progenitors. The self‐renewal capacity of MOSPD1‐null ESCs was normal and they exhibited no obvious defects in early germ layer specification nor in epithelial to mesenchymal transition (EMT), indicating that MOSPD1 functions after these key steps in the differentiation process. Mesenchymal stem cell (MSC)‐like cells expressing CD73, CD90, and CD105 were generated from MOSPD1‐null ESCs but their growth rate was significantly impaired implying that MOSPD1 plays a role in MSC proliferation. Phenotypic deficiencies exhibited by MOSPD1‐null ESCs were rescued by exogenous expression of MOSPD1, but not MOSPD3 indicating distinct functional properties of these closely related genes. Our in vitro studies were supported by RNA‐sequencing data that confirmed expression of Mospd1 mRNA in cultured, proliferating perivascular pre‐MSCs isolated from human tissue. This study adds to the growing body of knowledge about the function of this largely uncharacterized protein family and introduces a new player in the control of MSC proliferation and differentiation. Stem Cells 2015;33:3077–3086

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Section: Resultsmentioning

confidence: 99%

A Role for MOSPD1 in Mesenchymal Stem Cell Proliferation and Differentiation

et al. 2015

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“…The CD146 + subset of MSCs can longtime maintain the hematopoietic stem cells (HSCs) with engraftment and self-renewal ability [28]. In an experimental approach combining stringent cell purification by flow cytometry and differentiation in culture and in vivo, human CD146 + perivascular cells represent the ubiquitous ancestors of MSCs [29]. Additionally, CD146 has been reported to play a crucial role in the vascular development [30].…”

Section: Uca-pscs 3 Hmentioning

confidence: 99%

Different angiogenic potentials of mesenchymal stem cells derived from umbilical artery, umbilical vein and wharton’s jelly

Sun

Ding

2017

Fertility and Sterility

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“…Although present in the BM and other tissues such as the adipose, their precise location remained hampered by the lack of specific markers [47][48][49]. CD146 is used to identify MSPC in humans [50][51][52], and help demonstrate that these cells are perivascular similar to pericytes and that in addition to their ability to generate bone, cartilage and adipose when implanted in mice, MSPC also express HSC regulators such as Kit ligand and CXCL12 and could support HSC maintenance in vitro (reviewed in [53]). …”

Section: Mesenchymal Cells Mesenchymal Stromal Progenitor Cell (Mspc)mentioning

confidence: 99%