Peritrophic Matrices

Merzendorfer, Hans; Kelkenberg, Marco; Muthukrishnan, Subbaratnam

doi:10.1007/978-3-319-40740-1_8

Cited by 17 publications

(13 citation statements)

References 318 publications

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“…8), suggesting a possible degradation as parasite cysts transit towards this region. The remarkable PM expansion observed in some of the cysts at 11 dpi, where the PM layers remain intact despite containing several tightly packed trypanosomes, is indicative of a highly flexible PM rich in chitin fibres cross-linked to O-glycosylated peritrophins [14,40]. Whether parasite encapsulation within the PM is a novel tsetse defence mechanism to control trypanosome infection intensity remains to be elucidated, but one should note this process does not lead to self-cure.…”

Section: Do Serum Factors Influence Early Colonisation Of the Proventmentioning

confidence: 97%

Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus

et al. 2020

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Section: Do Serum Factors Influence Early Colonisation Of the Proventmentioning

confidence: 97%

Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus

et al. 2020

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“…8), suggesting a possible degradation as parasite cysts transit towards this region. The remarkable PM expansion observed in some of the cysts at 11 dpi, where the PM layers remain intact despite containing several tightly packed trypanosomes, is indicative of a highly flexible PM rich in β-chitin fibres cross-linked to O -glycosylated peritrophins [14, 40]. Whether parasite encapsulation within the PM is a novel tsetse defence mechanism to control trypanosome infection intensity remains to be elucidated, but one should note this process does not lead to self-cure.…”

Section: Resultsmentioning

confidence: 99%

Trypanosoma bruceicolonises the tsetse gut via an immature peritrophic matrix in the proventriculus

Rose

Dyer

Casas-Sánchez

et al. 2019

Preprint

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Projection (MIP) (top) and 3D reconstruction (bottom) of a trapped trypanosome. e, A PM sample from 130 a naïve fly depicting how this tissue looks under DIC and MIP after rendering from multiple z-sections, 131 whilst the orthogonal view shows the XZ/YZ planes of the folded PM section. f, DIC and MIP of an 132 infected PM sample containing trypanosome cysts, whilst the orthogonal XZ-YZ views show 133 trypanosomes trapped within PM layers. A second, smaller cyst-like structure can be seen in the XZ 134 orthogonal view at a North-West position to the bigger cyst. ES, ectoperitrophic space. L, lumen. Scale 135 bar 20µm. 136 137 Transmission electron microscopy (TEM) analyses of trypanosome-infected midguts 138 TEM was then used to better understand, at the ultrastructural level, the nature of the PM 139

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“…The insect midgut can be divided into three morphologically distinct regions: the anterior, middle and posterior regions (Harper & Granados, ; Merzendorfer et al ., ). The transcriptional expression patterns of proteins in the EcPS supported the notion that different regions of the midgut may have specialized functions.…”

Section: Discussionmentioning

confidence: 97%

“…In this study, we focused on the role of the EcPS as a major site for terminal digestion of food constituents in the insect midgut (Merzendorfer et al, 2016). There have been several proteomic studies of the EnPS and PM that identified major enzymes in the initial processing of food constituents (Nakazawa et al, 2004;Pauchet et al, 2008;Liu et al, 2010;Hu et al, 2012;Ponnuvel et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…These differences appear to be correlated with variations in gene expression by the midgut epithelial cells along the length of the midgut (Jasrapuria et al, 2010;Toprak et al, 2014). In addition, the midgut lumen can also be divided into three physiological compartments: the endoperitrophic space (EnPS); the introperitrophic space filled by the PM; and the ectoperitrophic space (EcPS) (Lehane, 1997;Merzendorfer et al, 2016). Each of these three compartments seems to coordinate with one another to ensure rapid and efficient digestion of the ingested food as it passes through the midgut.…”

Section: Introductionmentioning

confidence: 99%

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The physiological differentiation along the midgut of Bombyx mori – inspirations from proteomics and gene expression patterns of the secreted proteins in the ectoperitrophic space

Liu

Yang

et al. 2017

Insect Molecular Biology

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The ectoperitrophic space (EcPS) between the insect midgut epithelial cells and the peritrophic matrix is an unexplored, clean resource for concentrated proteins secreted by the midgut epithelial cells, which offers an ideal opportunity to uncover the midgut functions. In this study, we used Bombyx mori as a model organism and performed comparative proteomic analyses of the secreted proteins in the EcPS at the feeding and wandering stages. A total of 372 proteins were identified from both stages and 70 proteins were predicted to be secreted. Amongst these proteins, 17 secreted digestive proteins were identified and their temporal and spatial transcriptional expression patterns demonstrated that all these proteins were up-regulated at the feeding stage and differentially expressed in different parts of the midgut. Proteins with nutrient reservoir activity and defence activity were found to be up-regulated at the wandering stage. This work is the first to show the presence of digestive enzymes in the EcPS of the insect midgut using a proteomic approach, which provides evidence that suggests a physiological functional differentiation of the insect midgut. It is very clear that the EcPS undergoes dynamic changes in its composition of proteins in response to the changing needs of the insect at different developmental stages.

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Peritrophic Matrices

Cited by 17 publications

References 318 publications

Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus

Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus

Trypanosoma bruceicolonises the tsetse gut via an immature peritrophic matrix in the proventriculus

The physiological differentiation along the midgut of Bombyx mori – inspirations from proteomics and gene expression patterns of the secreted proteins in the ectoperitrophic space

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