Periostin induction in tumor cell line explants and inhibition of in vitro cell growth by anti-periostin antibodies

Tai, Isabella T.; Dai, Meiru; Chen, Lan Bo

doi:10.1093/carcin/bgi034

Cited by 91 publications

(90 citation statements)

References 14 publications

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“…By in situ hybridisation, we identified the tumour cells as the unique source of production of periostin while immunohistochemical analysis demonstrates the presence of periostin only in the juxtatumoral stroma. This is the first report where stromal localization of periostin is described as previous studies have reported localization of periostin in the cytoplasm of other types of cancer cells (Gillan et al, 2002;Bao et al, 2004;Shao et al, 2004;Kim et al, 2005;Tai et al, 2005). Although, we have occasionally detected a cytoplasmic immunoreactivity of periostin in PDAC cells, the stromal localization was always dominant.…”

Section: Discussionsupporting

confidence: 67%

“…A similar structure was reported for fasciclin I, a cell adhesion molecule involved in the development of the central nervous system of insects (Zinn et al, 1988), and for the big-h3 molecule, a secreted protein induced by TGF-b1 which promotes the attachment and spreading of fibroblasts (Skonier et al, 1992;LeBaron et al, 1995). Periostin was found to be overexpressed in various types of human cancer such as lung (Sasaki et al, 2001a), brain (Sasaki et al, 2002), ovary (Gillan et al, 2002), breast (Shao et al, 2004) and colon cancers Tai et al, 2005) and elevated levels have been detected in sera of patients with thymoma (Sasaki et al, 2001b), non-small lung carcinoma (Sasaki et al, 2001c) and breast cancer (Sasaki et al, 2003). Although these reports implicate periostin in tumour spread, the functional role of this protein is poorly described and recent publications report conflicting data.…”

Section: Introductionsupporting

confidence: 53%

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Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death: role of the β4 integrin and the PI3k pathway

Baril¹,

Gangeswaran²,

Mahon³

et al. 2006

Oncogene

256

244

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Pancreatic ductal adenocarcinoma is a devastating disease, characterized by a rapid progression and poor treatment response. Using gene expression profiling of pancreatic cancer tissues, we previously identified periostin as a potential diagnostic and therapeutic target. In this study, we report the overexpression of periostin in a larger set of pancreatic cancer tissues and show that although the periostin transcript is exclusively expressed in tumour cells, the protein product is only detected in the extracellular matrix adjacent to cancer cells. Using an enzyme-linked immunosorbent assay (ELISA) assay, we show significantly increased levels of periostin in the sera of pancreatic cancer patients compared to non-cancer controls. We demonstrate that periostin promotes the invasiveness of tumour cells by increasing the motility of cells without inducing expression of proteases, and enhances the survival of tumour cells exposed to hypoxic conditions. At the molecular level, we provide evidence that the a 6 b 4 integrin complex acts as the cell receptor of periostin in pancreatic cancer cells and that interaction promotes phosphorylation of focal adhesion kinase (FAK) and protein kinase B (AKT) though activation of the PI3 kinase pathway, but not the RAS/MEK/ERK pathway. These findings suggest an important role of periostin in pancreatic cancer and provide a rationale to study periostin for diagnostic and therapeutic applications.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionsupporting

confidence: 53%

Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death: role of the β4 integrin and the PI3k pathway

Baril¹,

Gangeswaran²,

Mahon³

et al. 2006

Oncogene

256

244

View full text Add to dashboard Cite

show abstract

“…Periostin is originally identified from osteoblasts and functions as a cell adhesion molecule for preosteoblast and to participate in osteoblast recruitment, attachment and spreading (Takeshita et al, 1993;Horiuchi et al, 1999). Previous studies showed that the expression of Periostin is upregulated in various types of cancer, including head and neck (Gonzalez et al, 2003), colon , Tai et al, 2005, breast , lung (Sasaki et al, 2001c) and ovarian cancer (Gillan et al, 2002). Here, we also found that 68% of OSCC cases expressed Periostin mRNA and 69% of OSCC cases expressed Periostin protein.…”

Section: Discussionmentioning

confidence: 99%

Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer

et al. 2006

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Oral squamous-cell carcinoma (OSCC) is one of the most common types of human cancer. Typically OSCC cells show persistent invasion that frequently leads to local recurrence and distant lymphatic metastasis. We previously identified Periostin as the gene demonstrating the highest fold change expression in the invasive clone by comparing the transcriptional profile of parent OSCC cell line and a highly invasive clone. Here, we demonstrated that Periostin overexpression enhanced invasiveness in oral cancer cell lines. To know the role of Periostin in invasion, angiogenesis and metastasis in OSCC cases, we first examined the expression of Periostin mRNA in 31 OSCC cases by RT -PCR and Periostin protein in 74 OSCC cases by immunohistochemistry. Then, we compared the Periostin expression with invasion pattern, metastasis and blood vessel density. Periostin mRNA and protein overexpression were frequently found in OSCC cases and Periostin expression was well correlated with the invasion pattern and metastasis. Moreover, blood vessel density of Periostin-positive cases was higher than those of Periostin-negative cases. Interestingly, recombinant Periostin enhanced capillary formation in vitro in a concentration-dependant manner. In summary, these findings suggest that Periostin may promote invasion and angiogenesis in OSCC, and that Periostin can be a strong marker for prediction of metastasis in oral cancer patients.

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“…Recent studies have shown that PN is highly expressed in various human cancers, and have suggested that PN promotes tumor growth and metastasis (3)(4)(5). Moreover, PN is believed to promote tumor growth and metastasis, and to be correlated with poor prognosis.…”

Section: Introductionmentioning

confidence: 99%

Role of periostin in cancer progression and metastasis: Inhibition of breast cancer progression and metastasis by anti-periostin antibody in a murine model

Kyutoku

Taniyama

Katsuragi³

et al. 2011

Int J Mol Med

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Abstract. Periostin (PN), a secreted adhesion-related protein expressed in the periosteum and periodontal ligaments, acts as a critical regulator of the formation and maintenance of bone and teeth, and also plays an important role in tumorigenesis. Although PN is highly expressed in various types of human cancers, its function is still unclear. In this study, we focused on the exon 17 region of PN, which is alternatively spliced out. To investigate the function of full-length PN with exon 17, we produced a neutralizing antibody (PN1-Ab) against the peptide encoded by exon 17. In vivo, administration of PN1-Ab significantly inhibited the growth of primary tumors as well as metastatic tumors, associated with prevention of bone destruction, resulting in increased survival of mice. consistent with in vivo data, the present in vitro study demonstrated that addition of full-length PN significantly inhibited cell adhesion and detached adherent cells, while PN1-Ab inhibited the action of PN in a dose-dependent manner. In addition, PN1-Ab significantly inhibited the proliferation, migration and invasion of 4T1 mouse breast cancer cells, which produced PN. Interestingly, PN1-Ab also inhibited the differentiation of osteoclasts. Overall, the present study demonstrated that PN plays a pivotal role in the progression and metastasis of breast cancer. Since administration of PN1-Ab prolonged cell survival through inhibition of the progression and metastasis of 4T1 cells, further development of the PN1-Ab such as generation of a humanized antibody may provide a new therapeutic agent against breast cancer.

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Periostin induction in tumor cell line explants and inhibition of in vitro cell growth by anti-periostin antibodies

Cited by 91 publications

References 14 publications

Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death: role of the β4 integrin and the PI3k pathway

Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death: role of the β4 integrin and the PI3k pathway

Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer

Role of periostin in cancer progression and metastasis: Inhibition of breast cancer progression and metastasis by anti-periostin antibody in a murine model

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