Periostin expression in cancer-associated fibroblasts of invasive ductal breast carcinoma

Ratajczak‐Wielgomas, Katarzyna; Grzegrzółka, Jędrzej; Piotrowska, Aleksandra; Gomułkiewicz, Agnieszka; Witkiewicz, Wojciech; Dzięgiel, Piotr

doi:10.3892/or.2016.5095

Cited by 41 publications

(60 citation statements)

References 53 publications

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“…In our recently published study (27), as well as in studies of other authors (20), a different localization of POSTN expression in invasive ductal carcinoma (IDC) e.g. in cancer-associated fibroblasts (CAFs) and in cancer cells was confirmed.…”

Section: Introductionsupporting

confidence: 61%

“…Existing literature data on the role of POSTN expression in breast cancer confirmed POSTN expression localized both in CAFs and in cancer cells, in which expression was usually found in 30-60% of analysed cases of cancer (19,20,27).…”

Section: Discussionmentioning

confidence: 69%

“…IHC reactions were assessed under a Bx41 light microscope (Olympus) by two independent pathologists blinded to the experimental and patient data. The expression of POSTN in cytoplasm was evaluated using the semi-quantitative IRS scale, according to Remmele and Stegner (30), which was used in our previous study (27). POSTN expression was evaluated on whole section (one slide per case).…”

Section: Methodsmentioning

confidence: 99%

“…Using laser capture microdissection technique (LCM), we were first to show POSTN expression at mRNA level in both of those cell types as well as statistically significant higher expression of mRNA POSTN in stromal cells relative to cancer cells (27). Furthermore, significantly increased expression of POSTN in CAFs in IDC relative to pre-invasive ductal breast carcinoma in situ (DCIS) was also noted, which may indicate the role of POSTN expression by CAFs in breast cancer progression (27). In view of the above facts and as one of the very first, in this study we have attempted to examine the importance of POSTN expression in IDC cancer cells only, in reference to clinicopathological data.…”

Section: Introductionmentioning

confidence: 99%

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Expression of periostin in breast cancer cells

Ratajczak‐Wielgomas

Grzegrzółka

Piotrowska

et al. 2017

International Journal of Oncology

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Periostin (POSTN) is a protein involved in multiple processes important for cancer development, both at the stage of cancer initiation and progression, as well as metastasis. The aim of this study was to determine the expression of POSTN in the cells of non-invasive ductal breast carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC) and to correlate it with clinicopathological data. Immunohistochemical studies (IHC) were conducted on 21 cases of fibrocystic breast change (FC), 44 cases of DCIS and 92 cases of IDC. POSTN expression at mRNA (real-time PCR) and protein level (western blot analysis) was also confirmed in selected breast cancer cell lines (MCF-7, SK-BR-3, MDA-MB-231 and BO2). Statistically significant higher level of POSTN expression in IDC and DCIS cancer cells compared to FC was noted. Also, the level of POSTN expression in the cytoplasm of IDC cells was shown to increase with the increasing degree of tumour malignancy (G) and significantly higher expression of POSTN was observed in each degree of tumour malignancy (G) relative to FC. Statistically significant higher POSTN expression was observed in tumours with estrogen receptor-negative (ER-) and progesterone receptor-negative (PR-) phenotypes in comparison to estrogen receptor-positive (ER+) and progesterone receptor-positive (PR+) cases, as well as significant negative correlation between POSTN expression in cancer cells and expression of ER and PR (p<0.05). Additionally, statistically significant differences in POSTN expression were shown between particular breast cancer cell lines, both at mRNA and protein level. Observed POSTN expression was the lowest in the case of MCF-7, and the highest in MDA-MB-231 and BO2 of the most aggressive potential clinically corresponding to G3 tumours. POSTN expression in the cytoplasm of IDC cancer cells may play an important role in cancer transformation mechanism.

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Section: Introductionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression of periostin in breast cancer cells

Ratajczak‐Wielgomas

Grzegrzółka

Piotrowska

et al. 2017

International Journal of Oncology

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“…The role of periostin in breast cancer has been described. Periostin is expressed in invasive ductal carcinoma cells [87]. Its expression increases with the cancer grade, suggesting that periostin may play a role in cancer progression [88].…”

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confidence: 99%

Vitamin K effects in human health: new insights beyond bone and cardiovascular health

et al. 2019

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Vitamin K is a cofactor for the function of the enzyme γ-glutamyl carboxylase, necessary for the activation of multiple vitamin K dependent-proteins. Vitamin K dependent-proteins (VKDPs) have important roles in bone health, vascular health, metabolism, reproduction as well as in cancer progression. Vitamin K deficiency is common in different conditions, including kidney disease, and it may influence the activity of VKDPs. This review discusses vitamin K status in human health and the physiologic and pathologic roles of VKDPs, beyond the established effects in skeletal and cardiovascular health.

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Cancer‐associated fibroblasts: Is it a key to an intricate lock of tumorigenesis?

Aden

Zaheer

Ahluwalia

et al. 2023

Cell Biology International

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The past few decades have witnessed a major leap in knowledge relating to the role of tumor microenvironment (TME) in carcinogenesis and evolving behavior of the tumor. Multiple factors within the TME modulate the cancer cells and the associated therapies. Stephen Paget first asserted that the microenvironment plays an important role in the growth of tumor metastasis. The most important player in the TME is cancer‐associated fibroblast (CAF) which significantly participates in the proliferation, invasion, and metastasis of tumor cells. CAFs show phenotypic and functional heterogeneity. Mostly CAFs originate from quiescent resident fibroblast or mesoderm‐derived precursor cells (mesenchymal stem cells), although several alternate sources of origin have been noted. However, due to lack of specific fibroblast‐restricted markers, it is very difficult to trace lineage and identify the biological origin of distinct subtypes of CAFs. CAFs are predominantly shown by several studies to mainly act as tumor‐promoting agents, however, tumor‐inhibiting actions are also being validated by several studies. A more objectified and comprehensive functional and phenotypic classification of CAF is required, which will help in better way for tumor management. Here, in this review, we have tried to review the current status of CAF origin, along with phenotypic and functional heterogeneity, and recent progress in CAF research.

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Periostin expression in cancer-associated fibroblasts of invasive ductal breast carcinoma

Cited by 41 publications

References 53 publications

Expression of periostin in breast cancer cells

Expression of periostin in breast cancer cells

Vitamin K effects in human health: new insights beyond bone and cardiovascular health

Cancer‐associated fibroblasts: Is it a key to an intricate lock of tumorigenesis?

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