Periodontitis causes abnormalities in the liver of rats

Vasconcelos, Any Carolina Cardoso Guimarães; Vasconcelos, Daniel Fernando Pereira; Silva, Felipe Rodolfo Pereira da; França, Luíz Felipe de Carvalho; Alves, Even Herlany Pereira; Lenardo, David Di; Pessoa, Larissa dos Santos; Novaes, Pedro Duarte; Barbosa, André Luiz dos Reis; Mani, Arya; Mariano, Flávia S.; Medeiros, Jand Venes R.; Oliveira, Aldeídia Pereira de

doi:10.1002/jper.18-0226

Cited by 23 publications

(45 citation statements)

References 47 publications

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“…The steatosis score was evaluated and classified according to the percentage of cells with steatosis, within a scale of 5 degrees: 0, absent or present in <5% of hepatocytes; Grade 1, ≥5% and <25%; Grade 2, ≥25% and <50%; Grade 3, ≥50% and <75% and Grade 4, ≥75%. The evaluation of inflammation and necrosis followed the following classification: 0: none; 1: <2 foci/field; 2: 2–4 foci / field; 3: >4 outbreaks/field …”

Section: Methodssupporting

confidence: 75%

High‐fat diet aggravates the liver disease caused by periodontitis in rats

Andrade

França

Pessoa

et al. 2019

Journal of Periodontology

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Background: Periodontitis is an inflammatory disease that causes periodontium and hepatic alterations. Liver disease is related to the intake of foods rich in fat and sugars (high-fat). The objective of this study was to evaluate whether a high-fat diet can aggravate the liver disease caused by ligature-induced periodontitis in rats.Methods: Twenty-one female rats were divided into three groups (n = 7 in each group): control; periodontitis (periodontitis induced with ligature) and high-fat + periodontitis (received hypercaloric diet and induction of periodontitis). The rats were submitted to the analyses of the following periodontal parameters: gingival bleeding index (GBI), probing pocket depth (PPD), tooth mobility (TM), and alveolar bone height. In the hepatic tissue, the levels of malondialdehyde (MDA), glutathione (GSH), total cholesterol, and myeloperoxidase activity (MPO) were measured. Liver samples were also histopathologically evaluated. Finally, blood levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose, total cholesterol, cholesterol high-density lipoprotein (HDL), and uric acid were measured. Results:The high-fat + periodontitis group presented an increase in the steatosis score (P < 0.05) for the histopathologic evaluation, when compared with the periodontitis group. MDA, uric acid and ALT levels also increased, whereas GSH and HDL levels showed lower values. Conclusion:A high-fat diet aggravates the liver disease caused by ligature-induced periodontitis in rats.

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Section: Methodssupporting

confidence: 75%

High‐fat diet aggravates the liver disease caused by periodontitis in rats

Andrade

França

Pessoa

et al. 2019

Journal of Periodontology

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“…A similar model has been used previously in several other studies (Dos Santos Carvalho et al, 2017;França et al, 2017;Vasconcelos et al, 2019). The animals were divided into three groups: control group (n = 8), which received no treatment or induction of periodontal disease; periodontitis (n = 8), and bromelain (n = 8), which received intraperitoneal treatment with bromelain (bromelain from pineapple stem, Sigma-Aldrich®, São Paulo, SP, Brazil) 15 mg/kg for 20 days + induction of periodontitis.…”

Section: Experimental Designmentioning

confidence: 99%

Bromelain reduces the non‐alcoholic fatty liver disease and periodontal damages caused by ligature‐induced periodontitis

Alves¹,

Carvalho²,

Silva

et al. 2020

Oral Diseases

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Objective: The objective of this research was to evaluate the effects of bromelain (derived from Ananas comosus) upon periodontitis in rats. Materials and Methods: Twenty-four rats were separated into groups: control, periodontitis, and bromelain treatment. Bromelain was administered daily by intraperitoneal injection for 20 days. Periodontitis was induced by ligature around the first molars. Oral parameters and blood biomarkers were measured. The histopathological evaluation of the hepatic tissue was performed. Bromelain treatment significantly reduced several oral inflammatory parameters, alveolar bone loss, and blood biomarkers compared to the rats on periodontitis. Results: Treatment with bromelain improved the steatosis score. Bromelain used in ligature-induced periodontitis in rats was able to reduce the oral inflammatory parameters Gingival Bleeding Index (GBI), tooth mobility (TM), probing pocket depth (PPD), malondialdehyde (MDA), alveolar bone height (ABH) and gingival myeloperoxidase (MPO) and blood parameters (cholesterol, triglycerides, alanine aminotransferase, and aspartate aminotransferase). Bromelain treatment reduced the impact of periodontitis, such as the reduction of hepatic steatosis and improvement in the dosages of MDA and GSH. Conclusion: Bromelain acts as a potential adjunct in the non-surgical treatment of periodontitis and, consequently, reduces the impact of periodontitis, acting as antiinflammatory and antioxidant. K E Y W O R D S alveolar bone loss, anti-inflammatory, antioxidant, periodontal diseases, proteinase How to cite this article: Alves EHP, Carvalho ADS, Silva FRP, et al. Bromelain reduces the non-alcoholic fatty liver disease and periodontal damages caused by ligature-induced periodontitis.

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“…Blocks of hepatic tissues, 6-9 samples per group, with 1 mm 3 of lateral and medial lobes were processed. 9 Ultrasections were examined using a TEM (JEM 1400; JEOL) at 80 kV. A digital camera system (Gatan) was used to capture the images.…”

Section: Ultrastructural Evaluation Of Hepatocytes By Transmission mentioning

confidence: 99%

“…2,3 Periodontitis causes damages not only to the periodontal tissues, but also alters other organs 4,5 and the liver. Studies have revealed an important relation between periodontitis and hepatic alterations [6][7][8][9] Investigations with different methods have sought to ameliorate periodontal damages, 10,11 but that is still a challenge to periodontology. This challenge opens up opportunities for the exploration of new drugs from natural origin as the αTPN, which is a volatile monoterpene, the main compound of the essential oils found in plants of the species: Croton sonderianus ('Marmeleiro black'), Eucalyptus globulus ('Eucalyptus'), and Melaleuca qinquenervia ('Niaouli').…”

Section: Introductionmentioning

confidence: 99%

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Alpha‐terpineol complexed with beta‐cyclodextrin reduces damages caused by periodontitis in rats

Vasconcelos

Vasconcelos²,

Silva³

et al. 2020

J of Periodontal Research

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Background and objective: This study aimed to assess the effectiveness of the treatment with alpha-terpineol (αTPN) complexed with beta-cyclodextrin (βCD) on oral, blood, and hepatic parameters in ligature-induced periodontitis. Material and Methods: Forty female rats were distributed among the following groups: control (vehicle solution), periodontitis (ligature + vehicle solution), 5 mg/kg of αTPN-βCD (ligature), and 25 mg/kg of αTPN-βCD (ligature). Compounds were administered daily via intraperitoneal injection over a 20-day period. Periodontitis was induced with the bilateral insertion of ligatures around the first lower molars of each rat. Oral parameters, as well as blood biomarkers, were measured: histopathological assessment of the hepatic tissue was carried out using light and transmission electron microscopy. Results: The treatment with αTPN-βCD significantly improved several oral parameters and blood biomarkers in comparison with rats with periodontitis. In addition, the treatment with αTPN-βCD significantly ameliorated the steatosis score and reduced the number of lipid droplets and the amount of foamy cytoplasm in the hepatocytes of rats with periodontitis. Conclusion: The results obtained suggest that the treatment with αTPN-βCD improves several oral and blood parameters in rats with experimental periodontitis. In addition, hepatic alterations caused by periodontitis were ameliorated in the rats treated with αTPN-βCD.

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Periodontitis causes abnormalities in the liver of rats

Abstract: Experimental periodontitis caused immunohistochemistry, histopathologic, ultrastructural, oxidative, and biochemical changes in the liver of rats.

Cited by 23 publications

References 47 publications

High‐fat diet aggravates the liver disease caused by periodontitis in rats

High‐fat diet aggravates the liver disease caused by periodontitis in rats

Bromelain reduces the non‐alcoholic fatty liver disease and periodontal damages caused by ligature‐induced periodontitis

Alpha‐terpineol complexed with beta‐cyclodextrin reduces damages caused by periodontitis in rats

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