Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model

Application of the cell sheet technique in tissue engineering

“…In the study by Iwata et al (20), three-layered PDL cell sheets were implanted, which were supported with woven polyglycolic acid, to dental root surfaces having three-wall periodontal defects in dogs. The bone defects were filled with porous β-tricalcium phosphate (β-TCP).…”

Stem Cell Regenerative Therapy in Oral and Maxillofacial Region: A Systematic Review.

“…Transplantation of PDL derived cells into animal models were shown to regenerate periodontal tissue. Iwata et al2009 (39) harvested and expanded primary canine PDL cells in vitro and also made into transplantable constructs containing Poly glycolic acid (PGA) Scaffold and PDL cell sheets. The transplantable constructs in combination with porous tricalcium phosphate (b-TCP) induced regeneration of periodontal structures, including alveolar bone, cementum, and periodontal fibers.…”

The Scope of Stem Cells in Periodontal Regeneration

“…To increase the strength and number of cells, a 3D culture model of multilayered cell sheet have so been developed [91,92]. Also more recently cell sheet fragments and cell sheet pellets have been developed to increase the efficacy of the cells transplanted especially in cases where the target sit is too small for the entire cell sheet [93,94].…”