‘Periodical’ Pattern of Kinetics of DNA Strand Break Repair Following γ-irradiation of Human T1-cells

Schneeweiss, F.; Tisljar-Lentulis, G.; Haustein, K.; Feinendegen, L. E.

doi:10.1080/09553008714551951

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…Our results obtained for 50 Gy are comparable to the experiments with human T 1 cells performed by Schneeweiss et al [8] who found a "periodical pattern" of repair kinetics with three to tour peaks. To elucidate the nature of the multicomponent repair curves, we have tried to act on the shape of the repair curves by using different experimental conditions:…”

Section: Discussionsupporting

confidence: 91%

“…Similar maxima ("waves") have been reported by several other authors after exposure to ionizing radiation. A temporary increase of the numbers of breaks during incubation of exposed mammalian ceils at 37 ~ was observed in some experiments by Williams and Little [9], Schneeweiss et al [7,8] and lliakis and Okayasu [18] in the radiation-sensi-tire mutant xrs-5. Cook and Brazell [2] observed such a pattern with nucleoid sedimentation.…”

Section: Discussionmentioning

confidence: 80%

“…It has been demonstrated by many authors [1][2][3][4][5][6][7][8] that the repair kinetics of DNA strand breaks induced by x-rays or y-rays reflects a multicomponent process.…”

Section: Introductionmentioning

confidence: 99%

“…Other authors described a "cyclic" repair process [1,9,10], a series of "waves" on the repair kinetic curves [2,4], or a "periodical" pattern of repair [8] indicating that DNA breaks can be formed by the action of enzymes with endonuclease activity. Schneeweiss et al [8] even observed four peaks on the repair kinetic curve by monitoring the total number of DNA-strand breaks in non-synchronized human T 1 cells according to the method developed by Birnboim and Jevcak [ 1 1 ].…”

Section: Introductionmentioning

confidence: 99%

“…Schneeweiss et al [8] even observed four peaks on the repair kinetic curve by monitoring the total number of DNA-strand breaks in non-synchronized human T 1 cells according to the method developed by Birnboim and Jevcak [ 1 1 ]. The "waves" on the repair curve were explained as a result of the interaction of two antagonistic processes: rejoining and generation of breaks during the repair of DNA damage.…”

Section: Introductionmentioning

confidence: 99%

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Complex repair kinetics of DNA strand breaks induced by γ-rays or UV radiation in Ehrlich ascites tumour cells

Mlejnek

Kozubek

1996

Radiat Environ Biophys

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Fluorometric analysis of DNA unwinding (FADU)-a sensitive technique for the detection of strand breaks in DNA-has been modified and used for the detailed investigation of repair kinetics of DNA-strand breaks arising under different conditions in Ehrlich ascites tumour (EAT) cells irradiated by gamma-rays or ultraviolet (UV) radiation. The repair kinetics of DNA-strand breaks induced in EAT cells by gamma-radiation was measured at radiation doses of 8, 20 and 50 Gy. We found complex repair curves in all cases, probably reflecting the combined processes of break rejoining and break generation during repair. In order to affect the above-mentioned processes, we have used different conditions of repair and different types of radiation. Lowering of the temperature of incubation and treating the cells by 5-fluoro-2'-deoxyuridine (FUdR) lead to complex changes of the repair curve with a reduced "wave" pattern. In order to change the type of damage to DNA, we used UV radiation (254 nm, 10 and 20 J/m2). Detailed studies of the repair kinetics showed that the repair curve for 10 J/m2 had a second maximum within 70 min after irradiation.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 80%

“…It has been demonstrated by many authors [1][2][3][4][5][6][7][8] that the repair kinetics of DNA strand breaks induced by x-rays or y-rays reflects a multicomponent process.…”

Section: Introductionmentioning

confidence: 99%