Periodic DNA Nanotemplates Synthesized by Rolling Circle Amplification

Beyer, Stefan; Nickels, Patrick; Simmel, Friedrich C.

doi:10.1021/nl050155a

Cited by 141 publications

(109 citation statements)

References 41 publications

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“…Due to the partially complementary sequences of these two DNA strands, they were hybridized to form a circular shape. After hybridization, the nick was enzymatically closed by T4 DNA ligase 35,36 .…”

Section: Rna Membrane Fabricationmentioning

confidence: 99%

Self-assembly of free-standing RNA membranes

et al. 2014

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RNA has emerged as a promising material for nanostructure and microstructure engineering. Although rare, some macroscopic RNA structures have also been constructed using lipid or polymer materials. Here, we report the first example of an enzymatically generated RNA membrane. This robust and free-standing RNA membrane has a macroscopic structure and is generated without any polymer support or complexation. Our RNA membrane is fabricated following two sequential processes, complementary rolling circle transcription and evaporation-induced self-assembly, and its structural and functional properties are rationally controlled by adjusting RNA base pairing. In this study, three types of RNA membranes are fabricated and are used to demonstrate potential applications.

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Section: Rna Membrane Fabricationmentioning

confidence: 99%

Self-assembly of free-standing RNA membranes

et al. 2014

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“…RCA is an enzymatic method that allows the fabrication of long, ss DNA of periodic sequence from cyclic templates. The resulting RCA products were employed to construct long tracks of proteins [89] and nanoparticles [90]. For the assembly of nanotubes, a circular ss DNA was combined with a primer strand and DNA polymerase Phi29 producing the periodic ss DNA Guide (Fig.…”

Section: Dna Origamimentioning

confidence: 99%

Drug delivery systems based on nucleic acid nanostructures

Vries

Zhang

Herrmann

2013

Journal of Controlled Release

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a b s t r a c t a r t i c l e i n f oThe field of DNA nanotechnology has progressed rapidly in recent years and hence a large variety of 1D-, 2D-and 3D DNA nanostructures with various sizes, geometries and shapes is readily accessible. DNA-based nanoobjects are fabricated by straight forward design and self-assembly processes allowing the exact positioning of functional moieties and the integration of other materials. At the same time some of these nanosystems are characterized by a low toxicity profile. As a consequence, the use of these architectures in a biomedical context has been explored. In this review the progress and possibilities of pristine nucleic acid nanostructures and DNA hybrid materials for drug delivery will be discussed. For the latter class of structures, a distinction is made between carriers with an inorganic core composed of gold or silica and amphiphilic DNA block copolymers that exhibit a soft hydrophobic interior.

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“…30 Similarly, Simmel et al obtained linear chains of NPs on longer scaffolds. 31 Kiehl et al has demonstrated the use of self-assembled 2D DNA lattices to organize AuNPs into periodic striped patterns (Fig. 11a).…”

Section: This Journal Is C the Royal Society Of Chemistry 2008mentioning

confidence: 99%