Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.01.038 View full text
A. Carl Sanchez, Jason A. Anspach, Tivadar Farkas

Abstract: The observed performance when using high efficiency, low volume (U)HPLC columns has and continues to be limited by the extra column dispersion introduced by the chromatographic system. Even with the latest UHPLC instruments the injection and detection systems are still major contributors to peak broadening, especially when using 50 mm × 2.1mm columns. A previously described injection technique now termed Performance Optimizing Injection Sequence (POISe) was shown to reduce or eliminate the impact of the inject…

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