Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey

Sağıroğlu, Pınar; Hasdemir, Ufuk; Gelmez, Gülşen Altınkanat; Aksu, Burak; Karatuna, Onur; Sõyletır, Gũner

doi:10.1016/j.bjm.2018.02.002

Cited by 17 publications

(10 citation statements)

References 43 publications

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“…However, first KPC-2-positive K. pneumoniae have been identified in Turkey, in 2014 [31]. Since then, sporadic KPC-producing K. pneumoniae was reported [32,33]. In the present study, we didn't detect bla KPC gene among tested carbapenem and colistin resistant K. pneumoniae isolates.…”

Section: Discussioncontrasting

confidence: 64%

“…In the present study, we didn't detect bla KPC gene among tested carbapenem and colistin resistant K. pneumoniae isolates. MBLs identified in K. pneumoniae mainly reported from Japan (IMP), Taiwan (IMP), Indian subcontinent (NDM), Balkan states (NDM), and Greece (VIM) [33]. Recent findings suggest that the Balkan states and the Middle East may act as secondary reservoirs of NDM-1 producers.…”

Section: Discussionmentioning

confidence: 99%

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Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

Hazırolan

Karagöz

2020

AMicr

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Carbapenemase-producing and colistin resistant Klebsiella pneumoniae has become a worldwide healthcare problem. This study describes molecular characterization of carbapenemase-producing and colistin resistant clinical K. pneumoniae isolates.A total of 93 non-replicate carbapenem and colistin resistant K. pneumoniae were recovered from clinical specimens in a university hospital during 2017–2019. Detection of blaOXA-48, blaKPC, blaNDM-1, blaIMP, blaVIM-1 and mcr-1, -2, -3, -4, -5, -6, -7, and -8 genes was performed by PCR. The bacterial isolates were assigned to clonal lineages by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).All isolates harbored blaOXA-48 and only two isolates harbored blaOXA-48, and blaNDM-1 genes together. In colistin resistant K. pneumoniae, mcr-1 was detected in two (2.1%) isolates. Ninety three isolates of K. pneumoniae were categorized into three clusters and five pulsotypes. MLST revealed two different sequence types, ST101 (89/93) and ST147 (4/93).In our study ST101 was found to be a significantly dominant clone carrying blaOXA-48 and among our strains a low frequency of mcr-1 gene was determined. The emergence of colistin resistance was observed in K. pneumoniae ST101 isolates. ST101 may become a global threat in the dissemination of carbapenem and colistin resistance.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

Hazırolan

Karagöz

2020

AMicr

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“…Disk diffusion susceptibility testing revealed zone inhibition diameters for ertapenem at 20 mm and imipenem at 26 mm, which were below the epidemiological (13)(14)(15)(16). Antimicrobial susceptibility testing was also performed on Mueller-Hinton agar supplemented with cloxacillin.…”

Section: Resultsmentioning

confidence: 99%

“…Carbapenemase production was investigated by lateral flow immunoassays using the RESIST-4 O.K.N.V. kit (CORIS BioConcept, Gembloux, Belgium) for the detection of KPC, NDM, VIM, and OXA-48-like enzymes and the NG-CARBA 5 test (NG Biotech, Guipry, France) for the detection of KPC, NDM, VIM, OXA-48-like, and IMP enzymes (13)(14)(15). Finally, genes for KPC, NDM, VIM, OXA-48-like, and IMP enzymes were sought using the Xpert Carba-R kit (Cepheid, Sunnyvale, CA) (16).…”

Section: Methodsmentioning

confidence: 99%

False-Positive Carbapenem-Hydrolyzing Confirmatory Tests Due to ACT-28, a Chromosomally Encoded AmpC with Weak Carbapenemase Activity from Enterobacter kobei

Jousset

Oueslati

Bernabeu

et al. 2019

Antimicrob Agents Chemother

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In Enterobacter cloacae complex (ECC), the overproduction of the chromosome-encoded cephalosporinase (cAmpC) associated with decreased outer membrane permeability may result in carbapenem resistance. In this study, we have characterized ACT-28, a cAmpC with weak carbapenemase activity, from a single Enterobacter kobei lineage. ECC clinical isolates were characterized by whole-genome sequencing (WGS), susceptibility testing, and MIC, and carbapenemase activity was monitored using diverse carbapenem hydrolysis methods. ACT-28 steady-state kinetic parameters were determined. Among 1,039 non-carbapenemase-producing ECC isolates with decreased susceptibility to carbapenems received in 2016-2017 at the French National Reference Center for antibiotic resistance, only 8 had a positive carbapenemase detection test (Carba NP). These eight ECC isolates were resistant to broad-spectrum cephalosporins due to AmpC derepression, showed decreased susceptibility to carbapenems, and were categorized as carbapenemase-producing Enterobacteriaceae (CPE) according to several carbapenemase detection assays. WGS identified a single clone of E. kobei ST125 expressing only its cAmpC, ACT-28. The bla ACT-28 gene was expressed in a wild-type and in a porin-deficient Escherichia coli background and compared to the bla ACT-1 gene. Detection of carbapenemase activity was positive only for E. coli expressing the bla ACT-28 gene. Kinetic parameters of purified ACT-28 revealed a slightly increased imipenem hydrolysis compared to that of ACT-1. In silico porin analysis revealed the presence of a peculiar OmpC-like protein specific to E. kobei ST125 that could impair carbapenem influx into the periplasm and thus enhance carbapenem-resistance caused by ACT-28. We described a widespread lineage of E. kobei ST125 producing ACT-28, with weak carbapenemase activity that can lead to false-positive detection by several biochemical and phenotypic diagnostic tests. E nterobacter cloacae complex (ECC) organisms are highly adapted to the hospital environment and are mostly responsible for hospital-acquired infections such as intravenous-catheter-related bacteremia, urinary tract infections (UTIs), and pulmonary infections (1). The ECC shows a genomic heterogeneity with 13 clusters based on their hsp60 sequences (2) and currently comprises seven different species (3): Enterobacter asburiae, E. cloacae, Enterobacter hormaechei, Enterobacter kobei, Enterobacter ludwigii, Enterobacter mori, and Enterobacter xiangfangensis.Enterobacter spp. naturally exhibit resistance to aminopenicillins, first-and second-

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“…In 2016, as part of the European Survey of Carbapenemase Producing Enterobacteriaceae (EuSCAPE) project, 155 carbapenemase suspected K. pneumoniae (n=134, 86.5%) and E. coli (n=21, 13.5%) isolates submitted from 18 centers in various regions of Turkey were investigated, but none was positive for KPC [20] . Recently, Sağıroğlu et al [29] reported the presence of KPC in K. pneumoniae strains isolated from two patients. In 2016, Kuskucu et al [30] reported two KPC-2-producing E. coli isolates in Turkey.…”

Section: Discussionmentioning

confidence: 99%

Investigation of blaKPC Gene by PCR in Carbapenem Resistant Escherichia coli and Klebsiella pneumoniae Clinical İsolates in a Tertiary Care Hospital in Turkey

Özyazici¹,

Özkaya²,

Kaya³

et al. 2019

mjima

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Introduction: One of the most common mechanisms in the development of carbapenem resistance is acquiring carbapenemases. A new and different carbapenemase, Klebsiella pneumoniae carbapenemase (KPC), has recently emerged in Turkey. Klebsiella pneumoniae carbapenemases are encoded by the bla KPC gene and can be transferred between different species. Reports showing the presence of bla KPC gene in isolates from Turkey are limited. The current study aimed to investigate the presence of bla KPC gene in carbapenem resistant K. pneumoniae and Escherichia coli isolates in a tertiary care hospital in Turkey. Materials and Methods: Patient samples from various clinical units in Adana Numune Training and Research Hospital between November 2013 and October 2014 were sent to our laboratory at the same hospital. K. pneumoniae and E. coli strains isolated from different clinical samples were identified using both conventional methods and the VITEK® 2.0 automated identification system (bioMérieux, France). Imipenem-cilastatin, meropenem, and ertapenem susceptibility was analyzed using a VITEK® 2.0 antibiotic susceptibility testing system (bioMérieux, France). The presence of blaKPC gene was investigated by polymerase chain reaction. Results: A total of 49 resistant K. pneumoniae and 33 E. coli isolates were included in the study. Most were isolated from urine specimens. Among the carbapenems we tested, highest resistance rates were to ertapenem (98.0% in K. pneumoniae and 84.8% in E. coli isolates). One K. pneumoniae isolate was found positive for the blaKPC gene. Conclusion: According to our literature survey, this study is among the first reports from Turkey presenting the isolation of KPC-producing K. pneumoniae. This finding indicates the need to monitor carbapenem resistance arising from bla KPC , which may spread horizontally. Moreover, surveillance of antibiotic sensitivity rates and observation of regional differences will be useful guidelines in determining infection control and antimicrobial use management policies.

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Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey

Cited by 17 publications

References 43 publications

Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

Emergence of carbapenemase-producing and colistin resistant Klebsiella pneumoniae ST101 high-risk clone in Turkey

False-Positive Carbapenem-Hydrolyzing Confirmatory Tests Due to ACT-28, a Chromosomally Encoded AmpC with Weak Carbapenemase Activity from Enterobacter kobei

Investigation of blaKPC Gene by PCR in Carbapenem Resistant Escherichia coli and Klebsiella pneumoniae Clinical İsolates in a Tertiary Care Hospital in Turkey

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