2018
DOI: 10.1016/j.bjm.2018.02.002 View full text |Buy / Rent full text
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Abstract: In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for blaKPC, blaIMP, blaVIM, blaNDM, and blaOXA-48. The rat… Show more

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“…However, first KPC-2-positive K. pneumoniae have been identified in Turkey, in 2014 [31]. Since then, sporadic KPC-producing K. pneumoniae was reported [32,33]. In the present study, we didn't detect bla KPC gene among tested carbapenem and colistin resistant K. pneumoniae isolates.…”
Section: Discussioncontrasting
“…In the present study, we didn't detect bla KPC gene among tested carbapenem and colistin resistant K. pneumoniae isolates. MBLs identified in K. pneumoniae mainly reported from Japan (IMP), Taiwan (IMP), Indian subcontinent (NDM), Balkan states (NDM), and Greece (VIM) [33]. Recent findings suggest that the Balkan states and the Middle East may act as secondary reservoirs of NDM-1 producers.…”
Section: Discussionmentioning
“…Disk diffusion susceptibility testing revealed zone inhibition diameters for ertapenem at 20 mm and imipenem at 26 mm, which were below the epidemiological (13)(14)(15)(16). Antimicrobial susceptibility testing was also performed on Mueller-Hinton agar supplemented with cloxacillin.…”
Section: Resultsmentioning
“…Carbapenemase production was investigated by lateral flow immunoassays using the RESIST-4 O.K.N.V. kit (CORIS BioConcept, Gembloux, Belgium) for the detection of KPC, NDM, VIM, and OXA-48-like enzymes and the NG-CARBA 5 test (NG Biotech, Guipry, France) for the detection of KPC, NDM, VIM, OXA-48-like, and IMP enzymes (13)(14)(15). Finally, genes for KPC, NDM, VIM, OXA-48-like, and IMP enzymes were sought using the Xpert Carba-R kit (Cepheid, Sunnyvale, CA) (16).…”
Section: Methodsmentioning
“…In 2016, as part of the European Survey of Carbapenemase Producing Enterobacteriaceae (EuSCAPE) project, 155 carbapenemase suspected K. pneumoniae (n=134, 86.5%) and E. coli (n=21, 13.5%) isolates submitted from 18 centers in various regions of Turkey were investigated, but none was positive for KPC [20] . Recently, Sağıroğlu et al [29] reported the presence of KPC in K. pneumoniae strains isolated from two patients. In 2016, Kuskucu et al [30] reported two KPC-2-producing E. coli isolates in Turkey.…”
Section: Discussionmentioning