Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine

Abstract

Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).

Evaluation to Select Tomato Genotypes with Big Fruit and Verification of Genetic Advance

“…The character of fruit weight per plant contained four transgressive segregant genotype, F3GH3-241, F3GH3-223, F3GH3-79, and F3GH3-123, while the numbers of fruit character contained only one genotype, F3GH3-123. Transgressive segregants verified by comparing the mean and variance [47,48] between selected genotype families and homozygous comparisons were also reported in Pisum sativum [28], and chili [29][30][31]. Verification results (Table 9) show the number of genotypes verified by putative transgressive segregants for the characters of flowering time, harvest time, fruit length, fruit diameter, fruit weight, fruit weight per plant, and the numbers of fruit (1, 1, 6, 5, 4, 4, 1 genotypes, respectively) of the ten observed genotypes.…”

Preferensi Petani Terhadap Karakteristik Varietas Unggul Baru Kedelai di Lahan Kering Kabupaten Pandeglang, Provinsi Banten

“…Selain faktor perluasan areal tanam dengan memanfaatkan lahan kering untuk meningkatkan produksi kedelai di Provinsi Banten, salah satu faktor yang sangat penting lainnya adalah tersedianya varietas unggul baru yang mampu beradaptasi pada lahan kering di Provinsi Banten. Varietas unggul merupakan salah satu kunci penting untuk meningkatkan produksi secara nasional (Yu & Chung, 2021;Wibisono et al, 2015;Wibisono et al, 2021;Wibisono et al, 2022). Dengan demikian, mendapatkan varietas unggul yang dapat beradaptasi dengan baik pada spesifik lokasi, khususnya di lahan kering Provinsi Banten menjadi penting untuk dilakukan pengkajian.…”

Diversity Analysis of 53 Soybean Accessions Introduced from China Based on Morphological Characteristics and SSR Markers

“…To achieve soybean self-sufficiency, soybean productivity needs to be increased through plant breeding to develop new superior varieties [11]. The development of new superior varieties can be achieved through various methods including artificial crossing [12,13], mutation induction [14][15][16][17], local variety purification [18], and utilization of introduced varieties [19].…”