Performance Evaluation of a Dedicated Preclinical PET/CT System for the Assessment of Mineralization Process in a Mouse Model of Atherosclerosis

Rucher, Guillaume; Camelière, Lucie; Fendri, Jihéne; Abbas, Ahmed M.; Dupont, Kevin; Kamel, Saı̈d; Delcroix, Nicolas; Dupont, Axel; Berger, L.; Manrique, Alain

doi:10.1007/s11307-018-1202-2

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…fluoride into mineralizing tissues [19][20][21][22][23] . These techniques allow for high resolution threedimensional imaging but are limited by the availability of specialized equipment, carry additional safety considerations, and require development of conjugated or radioactive reporters.…”

Section: Previous Researchers Have Used Single-photon Emission Comput...mentioning

confidence: 99%

Osterix‐mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model

et al. 2019

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Acknowledgements: ERE was supported in part by a grant from NIH (R01 GM 49039) and some of the experiments by a sponsored research agreement from Sanofi. The authors acknowledge the assistance of other members of the Edelman lab, the in vivo imaging core at MIT, and the bio-medical engineering department at Sanofi. Author Contributions Statement: SES, SU and LFC and NA designed the experiments. SES, SU, LFC, and DP performed experiments. SES and NA wrote the manuscript. SES and PFM generated the Osterix-mCherry mice. ERE and NA supervised the project. All authors discussed the results and reviewed the manuscript.

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Section: Previous Researchers Have Used Single-photon Emission Comput...mentioning

confidence: 99%

Osterix‐mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model

et al. 2019

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“…Key aspects for the critical appraisal of the applicability of PET are summarized with regards to the applicability of PET in vivo and ex vivo, the location and size of VC identifiable by PET, the repeatability of analysis in longitudinal studies, the compatibility with additional methods, and the personal and technical expanse. The exemplary workflow presented is based on the publications of [ 140 , 141 , 142 , 143 , 144 , 145 ]. …”

Section: Methods For the Detection Of Vascular Calcification In Rodentsmentioning

confidence: 99%

“…For imaging and quantification of calcification, the establishment of reconstruction parameters is decisive. A study evaluating PET performance found a significant impact on the assessment of the mineralization process by optimizing the reconstitution parameters in uremic and non-uremic rats [141]. In ApoE −/− mice on a high cholesterol diet, a 18 F-NaF PET/CT scan was conducted in vivo in isoflurane-anaesthetized mice with an intraperitoneal tracer injection, as well as ex vivo and calcification were found to progress over the time of high cholesterol feeding with results consistent between in vivo and ex vivo settings [142].…”

Section: Positron Emission Tomographymentioning

confidence: 99%

Vascular Calcification in Rodent Models—Keeping Track with an Extented Method Assortment

Herrmann

Gummi

Xia

et al. 2021

Biology

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Vascular calcification is a multifaceted disease and a significant contributor to cardiovascular morbidity and mortality. The calcification deposits in the vessel wall can vary in size and localization. Various pathophysiological pathways may be involved in disease progression. With respect to the calcification diversity, a great number of research models and detection methods have been established in basic research, relying mostly on rodent models. The aim of this review is to provide an overview of the currently available rodent models and quantification methods for vascular calcification, emphasizing animal burden and assessing prospects to use available methods in a way to address the 3R principles of Russel and Burch: “Replace, Reduce and Refine”.

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“…High spatial resolution is critical in preclinical positron emission tomography (PET) imaging where the ultimate goal would be to obtain an image definition in small animal models similar to that obtained in humans with clinical imaging. Since mice are three orders of magnitude smaller than humans, submillimetric (or submicroliter) spatial resolution is essential to discriminate small regions involved in tumor heterogeneity (Buvat et al 2015), inflammation processes (Rucher et al 2018), neurodegenerative disease progression (Dubois et al 2016) or cardiac gated studies (Croteau et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Performance evaluation of the mouse version of the LabPET II PET scanner

Gaudin¹,

Thibaudeau²,

Arpin³

et al. 2021

Phys. Med. Biol.

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The LabPET II is a new positron emission tomography technology platform designed to achieve submillimetric spatial resolution imaging using fully pixelated avalanche photodiodes-based detectors and highly integrated parallel front-end processing electronics. The detector was designed as a generic building block to develop devices for preclinical imaging of small to mid-sized animals and for clinical imaging of the human brain. The aim of this work is to assess the physical characteristics and imaging performance of the mouse version of LabPET II scanner following the NEMA NU4-2008 standard and using high resolution phantoms and in vivo imaging applications. A reconstructed spatial resolution of 0.78 mm (0.5 μ l) is measured close to the center of the radial field of view. With an energy window of 350 650 keV, the system absolute sensitivity is 1.2% and its maximum noise equivalent count rate reaches 61.1 kcps at 117 MBq. Submillimetric spatial resolution is achieved in a hot spot phantom and tiny bone structures were resolved with unprecedented contrast in the mouse. These results provide convincing evidence of the capabilities of the LabPET II technology for biomolecular imaging in preclinical research.

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Performance Evaluation of a Dedicated Preclinical PET/CT System for the Assessment of Mineralization Process in a Mouse Model of Atherosclerosis

Abstract: Optimizing reconstruction parameters significantly impacted on the assessment of mineralization process in a preclinical model of accelerated atherosclerosis using Na[F]F PET. In addition, improving the CT resolution was associated with a dramatic radiation dose increase.

Cited by 4 publications

References 31 publications

Osterix‐mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model

Osterix‐mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model

Vascular Calcification in Rodent Models—Keeping Track with an Extented Method Assortment

Performance evaluation of the mouse version of the LabPET II PET scanner

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