“…Vials containing only inoculum were used for blank correction. Once filled, the vials were sealed with rubber stoppers and aluminum caps, shaken and placed in an incubator (Shel Lab, Sheldon Manufacturing, Inc., Cornelius, OR, USA) at 39 ± 0.5 • C. The gas pressure in the headspace was measured manually by inserting a sterile needle connected to the pressure transducer at 3,6,9,12,15,18,21,24,30,36,48,60,72,96, and 144 h after inoculation. The volume of gas produced was estimated from the pressure values as proposed by López et al [13].…”