Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever

Napoleão-Pêgo, Paloma; Carneiro, Flavia Raquel Gonçalves; Durans, Andressa da Matta; Gomes, Larissa Rodrigues; Morel, Carlos Médicis; Provance, David William; De-Simone, Salvatore G.

doi:10.1038/s41598-021-94817-x

Cited by 12 publications

(9 citation statements)

References 60 publications

(55 reference statements)

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“…The IgG immunodominant epitopes were the same as the ones previously described in Asian, European, or North American epitope mapping studies 1 – 11 . Taken together, the immune epitopes found in this study may help the development of epitope-based serological diagnostic assays or effective vaccine candidates 12 .…”

Section: Introductionmentioning

confidence: 69%

Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts

Vigan-Womas

Spadoni

Poiret

et al. 2023

Sci Rep

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Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development.

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Section: Introductionmentioning

confidence: 69%

Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts

Vigan-Womas

Spadoni

Poiret

et al. 2023

Sci Rep

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“…The performance of selected epitopes strongly supports the continued use of these epitopes with a chimeric multi-epitope protein [59] as a target in more sensitive and fast diagnostic tests and suggests that the antigen may be eligible to enter phase IIB studies [60].…”

Section: Discussionmentioning

confidence: 77%

Fine Epitope Mapping of the <em>Vibrio cholera</em> Toxins A, B, and P and an ELISA Assay

De-Simone¹,

Napoleão-Pêgo²,

Gonçalves³

et al. 2022

Preprint

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Oral immunization with the choleric toxin (CT) elicits a high level of protection against its enterotoxin activities and can control cholera in endemic settings. However, the complete B-cell epitope map of the CT responsible for protection remains to be clarified. Here, we have mapped the B-cell linear epitopes of the three chains of the CT protein (CTP) prepared by Spot synthesis. The immunoreactivity of sera from mice immunized with an oral, inactivated vaccine (Schankol&dagger;™) was measured against membrane-bound peptides for mapping. Results: Eighteen IgG epitopes were identified; eight in the CTA, three in the CTB, and seven in the protein P. Three epitopes, TQTGFVRHDDGYVST (aa 66-77, Vc/TxA-3), KNGAIFQVE VPGSQN (aa 64-78, Vc/TxB-11), and LNDEHK (aa 90-95, Vc/TxP-16), were chosen to synthesize a multiple antigen peptide that was used to coat ELISA plates to screen immunized mouse sera as a test for an in vitro diagnostics for cholera. Conclusion: Vaccination with inactive CT-generated antibodies against multiple linear epitopes and the selected epitopes can be used to construct immunological reagents for rapid serological diagnosis of cholera with high sensitivity and specificity.

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“…Tetanus is diagnosed by the patient's history and clinical signs [63] since there are no universal disposable laboratory tests [64]. Thus, concerning the diagnosis, our ELISA-peptide results using the MAP4 epitope peptides revealed that all four epitopes appropriately discriminated between negative and positive samples (p < 0.0001).…”

Section: Discussionmentioning

confidence: 86%

High-Throughput IgG Epitope Mapping of Tetanus Neurotoxin: Implications for Immunotherapy and Vaccine Design

De-Simone¹,

Napoleão-Pêgo²,

Lechuga³

et al. 2023

Preprint

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Background: Tetanus is an acute, fatal disease caused by exotoxins released from Clostridium tetani during infections. A protective humoral immune response can be induced by vaccinations with pediatric and booster combinatorial vaccines that contain inactivated tetanus neurotoxin (TeNT) as a major antigen. Although some epitopes in NeNT have been described using various ap-proaches, a comprehensive list of its antigenic determinants that are involved with immunity have not been elucidated. To that end, a high resolution analysis of the linear B-cell epitopes in NeNT was performed using antibodies generated in vaccinated children. Methods: Two hundred sixty-four peptides that cover the entire coding sequence of TeNT protein were prepared in situ on a cellulose membrane by SPOT synthesis and probed with sera from children vaccinated (ChVS) with a triple DTP-vaccine to map continuous B-cell epitopes that were further characterized and validated by immunoassays. Results: Forty-three IgG epitopes were identified. Four (TT-215-218) were chemically synthesized as Multiple Antigen Peptides (MAPs) and used in peptide ELISAs to screen post-pandemic DTP vaccinations. The assay displayed a high performance with high sensitivity (99.99%) and specificity (100%). Conclusions: The complete map of linear IgG epitopes induced by vaccination with inactivated TeNT highlights three key epitopes involved in the ef-ficacy of the vaccine. Antibodies against epitope TT-8/G can block enzymatic activity, and those against epitopes TT-40/G and TT-42/G can interfere with TeNT binding to neuronal cell receptors. We further show that four of the epitopes identified can be employed in peptide ELISAs to assess vaccine coverage. Overall, the data suggest a set of select epitopes to engineer new, directed vaccines

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Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever

Cited by 12 publications

References 60 publications

Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts

Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts

Fine Epitope Mapping of the <em>Vibrio cholera</em> Toxins A, B, and P and an ELISA Assay

High-Throughput IgG Epitope Mapping of Tetanus Neurotoxin: Implications for Immunotherapy and Vaccine Design

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