Perfluorocarbon Liquid: Its Application in Vitreoretinal Surgery and Related Ocular Inflammation

Qi, Yu; Liu, Kun; Su, Li; Xia, Xin; Xu, Xun

doi:10.1155/2014/250323

Cited by 52 publications

(57 citation statements)

References 66 publications

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“…Perfluorocarbons have been intensively used in biomedical applications for almost two decades for biomedical imaging under the form of nano‐emulsions or even biomedical therapeutics as an artificial, intravascular oxygen carrier . Other biomedical applications include lung surfactant replacement and ophthalmologic aids . For cell and tissue culture systems, a series of benefits of using perfluorocarbons have been identified which include easy sterilisation, chemical and biological inertness, recoverability and recyclability, scavengers for gaseous cellular products and the ability to form two‐phase liquid/liquid systems …”

Section: Introductionmentioning

confidence: 99%

“…26,27 Other biomedical applications include lung surfactant replacement 27,28 and ophthalmologic aids. 27,29 For cell and tissue culture systems, a series of benefits of using perfluorocarbons have been identified which include easy sterilisation, chemical and biological inertness, recoverability and recyclability, scavengers for gaseous cellular products and the ability to form two-phase liquid/liquid systems. 30,31 When anchorage-dependent cells are inoculated in the perfluorocarbon/medium two-phase system, cells migrate to the interface where they attach, spread and grow.…”

Section: Introductionmentioning

confidence: 99%

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Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system

Hanga

Murasiewicz

Pacek

et al. 2017

J. Chem. Technol. Biotechnol

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BACKGROUNDHuman mesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerative medicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors that could determine specialised tissue regeneration. To date, hMSCs have been successfully cultured in vitro on substrates such as polystyrene dishes (TCPS) or microcarriers. However, hMSC sub‐cultivation and harvest typically employs proteolytic enzymes that act by cleaving important cell membrane proteins resulting in long‐term cell damage. In a process where the cells themselves are the product, a non‐enzymatic and non‐damaging harvesting approach is desirable.RESULTSAn alternative system for hMSC expansion and subsequent non‐enzymatic harvest was investigated here. A liquid/liquid two‐phase system was proposed, comprising a selected perfluorocarbon (FC40) and growth medium (DMEM). The cells exhibited similar cell morphologies compared with TCPS. Moreover, they retained their identity and differentiation potential post‐expansion and post‐harvest. Further, no significant difference was found when culturing hMSCs in the culture systems prepared with either fresh or recycled FC40 perfluorocarbon.CONCLUSIONSThese findings make the FC40/DMEM system an attractive alternative for traditional cell culture substrates due to their ease of cell recovery and recyclability, the latter impacting on overall process costs. © 2017 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system

Hanga

Murasiewicz

Pacek

et al. 2017

J. Chem. Technol. Biotechnol

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“…Subretinal PFCL is a relatively rare but serious complication secondary to surgical repair of rhegmatogenous retinal detachment. Temporary exposure to PFCL has been reported to be clinically safe, however, retained PFCL has the potential toxic effects on various structures of the retina [ 2 ]. Actually, it is optimal to notice the PFCL bubbles intraoperatively and remove subretinal PFCL immediately.…”

Section: Discussionmentioning

confidence: 99%

Surgical removal of submacular perfluorocarbon liquid with a 38-gauge flexible cannula combined with internal limiting membrane peeling and intravitreal air tamponade: a case series

et al. 2018

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BackgroundTo report a case series in which a modified technique was used to remove retained submacular perfluorocarbon liquid (PFCL) secondary to vitreoretinal surgery for rhegmatogenous retinal detachment.Case presentationFour patients who had undergone pars plana vitrectomy for rhegmatogenous retinal detachment were further treated with surgical intervention because of retained submacular PFCL. With a three-port pars plana approach, after the internal limiting membrane peeling with indocyanine green staining, a 38-gauge flexible cannula was used to aspirate the submacular perfluorocarbon bubble, followed by fluid-air exchange and air injection into vitreous cavity. Submacular perfluorocarbon liquid was removed successfully and visual acuity had an improvement in all cases.ConclusionThe surgical removal of retained submacular PFCL using a 38-gauge flexible cannula combined with internal limiting membrane peeling and intravitreal air tamponade may provide anatomical and visual satisfactory outcomes.Electronic supplementary materialThe online version of this article (10.1186/s12886-018-0798-y) contains supplementary material, which is available to authorized users.

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“…At US, PFCL retention is seen as echogenic images with marked reverberation artefacts (Fig. 25 ) [ 33 ].…”

Section: Pathologic Us Findingsmentioning

confidence: 99%

Ocular ultrasonography focused on the posterior eye segment: what radiologists should know

et al. 2016

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Ocular B-mode ultrasonography (US) is an important adjuvant for the clinical assessment of a variety of ocular diseases. When ophthalmoscopy is not possible, mainly due to opacification of the transparent media (e.g., mature cataract or vitreous haemorrhage), US can guide the ophthalmologist in diagnosing disease and choosing treatment. The superficial location and cystic structure of the eye make US ideal for imaging of the eye. Moreover, dynamic study helps distinguish between various conditions that would otherwise be difficult to differentiate in some clinical setting, such as vitreous, retinal, and choroidal detachment. US is also good technique for detecting other pathologic conditions such as lens dislocation, vitreous haemorrhage, asteroid hyalosis, optic disc drusen, and tumors (e.g., choroidal melanoma, metastases, hemangioma). An understanding of the basic anatomy of the eye, the US technique, and common entities that affect the ocular globe will allow radiologists to offer this valuable imaging modality to patients and referring clinicians. This article focuses on the US anatomy and pathologic conditions that affect the posterior ocular segment.Teaching points• US is specially indicated when ocular fundus cannot be assessed on ophthalmoscopy.• Multipurpose equipment with high-frequency transducers is optimal for imaging the eye.• Ultrasound can reliably depict ocular anatomy and pathology as detachments and tumours.• Dynamic examination is vital for distinguishing certain pathologic conditions as detachments.Electronic supplementary materialThe online version of this article (doi:10.1007/s13244-016-0471-z) contains supplementary material, which is available to authorized users.

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Perfluorocarbon Liquid: Its Application in Vitreoretinal Surgery and Related Ocular Inflammation

Cited by 52 publications

References 66 publications

Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system

Expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs) using a two-phase liquid/liquid system

Surgical removal of submacular perfluorocarbon liquid with a 38-gauge flexible cannula combined with internal limiting membrane peeling and intravitreal air tamponade: a case series

Ocular ultrasonography focused on the posterior eye segment: what radiologists should know

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