Penelitian ini bertujuan melakukan optimalisasi keberadaan Pelabuhan Perikanan Labuan agar dapat menjadi pengungkit perekonomian daerah. Penelitian dilakukan pada bulan September sampai dengan November 2021 dengan pendekatan kualitatif dan kuantitatif (mix method). Pengumpulan data primer yang terkait dengan persepsi setiap pemangku kepentingan (stakeholder) dilakukan melalui wawancara dan diskusi kelompok terpumpun (focus group discussion), sedangkan analisis data menggunakan MACTOR (matrix of alliance, conflicts, tactics, objectives and recommendations). Hasil penelitian menunjukkan bahwa terdapat sebelas pemangku kepentingan yang berperan dalam pengembangan Pelabuhan Perikanan Labuan dengan empat pemangku kepentingan di antaranya, yakni UPTD PPP, nelayan, pengelola TPI, dan kelompok pengolah hasil perikanan, memiliki tingkat ketergantungan serta tingkat pengaruh yang kuat terhadap keberhasilan pengembangan Pelabuhan Perikanan Labuan. Seluruh pemangku kepentingan bersepakat bahwa tujuan pengembangan pelabuhan perikanan diarahkan pada peningkatan peran UPTD PPP dalam pencapaian tujuan pembangunan, keamanan dan keselamatan pelayaran, peningkatan ekonomi masyarakat, peningkatan pendapatan desa, dan fasilitasi bagi nelayan. Keberadaan pedagang kaki lima di wilayah pelabuhan akan menimbulkan permasalahan jika tidak dikelola dengan baik. Rekomendasi yang diberikan adalah perlunya optimalisasi peran seluruh pemangku kepentingan yang terkait dengan pengembangan Pelabuhan Perikanan Labuan, sinkronisasi program kerja dari seluruh pemangku kepentingan untuk mengembangkan pelabuhan perikanan di Labuan agar tujuan pengembangannya tercapai dan memberikan manfaat bagi masyarakat, serta perlunya pelibatan institusi desa melalui pengadaan lahan bagi pedagang kaki lima agar keberadaannya tidak mengganggu fungsi pelabuhan. Title: Stakeholders Analysis for the Development of Labuan Fishery Port to Improve Economy of the Community This study aims to optimize the existence of the Labuan Fishing Port so that can be leveraged for the regional economy. This research was conducted from September to November 2021 with a qualitative and quantitative method approach (mix method). Primary data collection related to the perception of each stakeholder is carried out through interviews and focus group discussions while data analysis used MAXTOR (matrix of alliance, conflicts, tactics, objectives and recommendations) to see the role of stakeholders in achieving outcomes directly or indirectly. The results showed that there were eleven stakeholders who played a role in the development of the Labuan Fishery Port and four of theme, namely UPTD PPP, fishermen, TPI managers and fishery product processing groups, had a high level of dependence and a strong level of influence on the success of fishing port development. All stakeholders agree that the purpose of developing fishing ports is directed at increasing the role of UPTD PPP in achieving development goals, shipping security and safety, improvement of the community’s economy, increase in village income and fisherman facilitation. The existence of street vendors in the port area will cause problems if not managed properly. The recommendations given are the need to optimize the role of all relevant stakeholders in the development of the Labuan Fishing Port, to synchronize the work programs of all stakeholders to develop a fishing port in Labuan so that its development goals are achieved and provide benefits to the community and the need to involve village institutions through land acquisition for street vendors in the port area.