Peptide conjugation enhances the cellular co-localization, but not endosomal escape, of modular poly(acrylamide-co-methacrylic acid) nanogels

Clegg, John R.; Sun, Jessie A.; Gu, Joann; Venkataraman, Abhijeet; Peppas, Nicholas A.

doi:10.1016/j.jconrel.2020.10.045

Cited by 8 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concentrations from 10 μm to 3.21 mm were reported to study systems codelivering calcein, [167,170,191] with the majority of studies applying concentrations between 161 and 322 μm. [168,173,[177][178][179][180][181][182][183][184][185][186]190,192] Using a calcein concentration of 161 μm, Deshpande et al have shown that poly(N-isopropylacrylamide nanogels with poly-𝜖-lysine can release calcein in cervical cancer cells. [168] Similar concentrations were used to study nanoparticles with hydrophobic components.…”

Section: Simultaneous and Sequential Addition Of Nanocarrier And Calc...mentioning

confidence: 99%

“…Concentrations from 10 µ m to 3.21 m m were reported to study systems codelivering calcein, [ 167,170,191 ] with the majority of studies applying concentrations between 161 and 322 µ m . [ 168,173,177–186,190,192 ]…”

Section: Calcein Release Studied Within Cellsmentioning

confidence: 99%

“…[ 181 ] Calcein concentrations in a similar range have also been shown to be useful for studying CPPs. [ 184 ] As an example, Salomone and colleagues showed that a fusion peptide from the arginine‐rich TAT motif with additional residues of cecropin‐A and melittin can efficiently release calcein within 30 min with a calcein concentration of 250 µ m . [ 183 ] However, endosomal escape could also be detected with significantly lower calcein doses of 40 µ m or less.…”

Section: Calcein Release Studied Within Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Hausig‐Punke

Richter

Hoernke

et al. 2022

Macromolecular Bioscience

View full text Add to dashboard Cite

Crossing the cellular membrane and delivering active pharmaceuticals or biologicals into the cytosol of cells is an essential step in the development of nanomedicines. One of the most important intracellular processes regarding the cellular uptake of biologicals is the endolysosomal pathway. Sophisticated nanocarriers are developed to overcome a major hurdle, the endosomal entrapment, and delivering their cargo to the required site of action. In parallel, in vitro assays are established analyzing the performance of these nanocarriers. Among them, the release of the membrane‐impermeable dye calcein has become a popular and straightforward method. It is accessible for most researchers worldwide, allows for rapid conclusions about the release potential, and enables the study of release mechanisms. This review is intended to provide an overview and guidance for scientists applying the calcein release assay. It comprises a survey of several applications in the study of endosomal escape, considerations of potential pitfalls, challenges, and limitations of the assay, and a brief summary of complementary methods. Based on this review, it is hoped to encourage further research groups to take advantage of the calcein release assay for their own purposes and help to create a database for more efficient cross‐correlations between nanocarriers.

show abstract

Section: Simultaneous and Sequential Addition Of Nanocarrier And Calc...mentioning

confidence: 99%

Section: Calcein Release Studied Within Cellsmentioning

confidence: 99%

Section: Calcein Release Studied Within Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Hausig‐Punke

Richter

Hoernke

et al. 2022

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Enzymes present in the body are suitable analytes, which can induce a hydrogel’s response in select environments for targeted drug-delivery applications. Typically, this is accomplished using peptide sequences that are degraded by the target enzyme [ 161 , 162 ]. Kopecek and colleagues [ 163–172 ] have developed and tested N-(2-hydroxypropyl)methacrylamide hydrogels containing bound drug molecules attached via oligopeptide cross-links.…”

Section: Stimuli-responsive Biomedical Systemsmentioning

confidence: 99%

Advanced biomedical hydrogels: molecular architecture and its impact on medical applications

Peters

Wechsler

Peppas

2021

Regenerative Biomaterials

View full text Add to dashboard Cite

Hydrogels are crosslinked polymeric networks swollen in water, physiological aqueous solutions, or biological fluids. They are synthesized by a wide range of polymerization methods that allow for the introduction of linear and branched units with specific molecular characteristics. In addition, they can be tuned to exhibit desirable chemical characteristics including hydrophilicity or hydrophobicity. The synthesized hydrogels can be anionic, cationic, or amphiphilic, and can contain multifunctional crosslinks, junctions or tie points. Beyond these characteristics, hydrogels exhibit compatibility with biological systems, and can be synthesized to render systems that swell or collapse in response to external stimuli. This versatility and compatibility have led to better understanding of how the hydrogel’s molecular architecture will affect their physicochemical, mechanical, and biological properties. We present a critical summary of the main methods to synthesize hydrogels which define their architecture, and advanced structural characteristics for macromolecular/biological applications.

show abstract

“…Researchers have explored the possible conjugation between peptide precursors and polymers to form smart NPs for sequential targeting or enhance the efficacy of encapsulated payloads in vivo [101,102]. Most recently, CC-9 peptide conjugated to poly(acrylamide-co-methacrylic acid) nanogels showed enhanced co-localization with SW-48 colon cancer cell lines to augment colon cancer targeting abilities [103]. To date, various peptides (e.g., papain, albumin, arginine-glycine-aspartic acid (RGD), and azido-bi-functionalized peptides) have been used in the development of peptide-polymer conjugates [101,[104][105][106].…”

Section: Peptide-polymer Conjugatesmentioning

confidence: 99%

Development of Polymer-Assisted Nanoparticles and Nanogels for Cancer Therapy: An Update

Neerooa

Ooi

Shameli

et al. 2021

Gels

View full text Add to dashboard Cite

With cancer remaining as one of the main causes of deaths worldwide, many studies are undergoing the effort to look for a novel and potent anticancer drug. Nanoparticles (NPs) are one of the rising fields in research for anticancer drug development. One of the key advantages of using NPs for cancer therapy is its high flexibility for modification, hence additional properties can be added to the NPs in order to improve its anticancer action. Polymer has attracted considerable attention to be used as a material to enhance the bioactivity of the NPs. Nanogels, which are NPs cross-linked with hydrophilic polymer network have also exhibited benefits in anticancer application. The characteristics of these nanomaterials include non-toxic, environment-friendly, and variable physiochemical properties. Some other unique properties of polymers are also attributed by diverse methods of polymer synthesis. This then contributes to the unique properties of the nanodrugs. This review article provides an in-depth update on the development of polymer-assisted NPs and nanogels for cancer therapy. Topics such as the synthesis, usage, and properties of the nanomaterials are discussed along with their mechanisms and functions in anticancer application. The advantages and limitations are also discussed in this article.

show abstract

Peptide conjugation enhances the cellular co-localization, but not endosomal escape, of modular poly(acrylamide-co-methacrylic acid) nanogels

Cited by 8 publications

References 49 publications

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters

Advanced biomedical hydrogels: molecular architecture and its impact on medical applications

Development of Polymer-Assisted Nanoparticles and Nanogels for Cancer Therapy: An Update

Contact Info

Product

Resources

About