Peptide antibiotics in mast cells of fish

Silphaduang, Umaporn; Noga, Edward J.

doi:10.1038/35104690

Cited by 329 publications

(339 citation statements)

References 11 publications

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“…Furthermore, the present study has shown that in addition to their constitutive production in the liver, both LEAP-2 peptides are induced in intestine and skin following bacterial exposure. It has been demonstrated that eosinophilic granule cells in some mucosal sites of hybrid striped bass contain AMPs although their identity is still unknown (Silphaduang and Noga, 2001). Whilst such cells could be responsible for LEAP-2 production in intestine and skin of bacterially challenged rainbow trout the liver lacks such cells and so either a different cell type produces LEAP-2 at both sites or LEAP-2 is produced by more than one cell type in trout.…”

Section: Discussionmentioning

confidence: 99%

Discovery and characterization of two types of liver-expressed antimicrobial peptide 2 (LEAP-2) genes in rainbow trout

Zhang

Zou

Chang

et al. 2004

Veterinary Immunology and Immunopathology

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Section: Discussionmentioning

confidence: 99%

Discovery and characterization of two types of liver-expressed antimicrobial peptide 2 (LEAP-2) genes in rainbow trout

Zhang

Zou

Chang

et al. 2004

Veterinary Immunology and Immunopathology

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“…Previous studies of vertebrate antimicrobial compounds indicate that these substances are not specific, with inhibitory effects demonstrated on a broad range of microbes (Zasloff 1987(Zasloff , 2002Boman 1995;Cole et al 1997;LeMaitre et al 1997;Silphaduang & Noga 2001). Because a limited amount of mucus could be acquired from each E. crossopterum male, and relatively small population sizes in each stream limited the number of males that could be captured, we directed antimicrobial assays at bacterial and fungal strains that represented common characteristics of the microbial community.…”

Section: (C) Microbial Assaysmentioning

confidence: 99%

“…Oncorhynchus mykiss (Austin & McIntosh 1988); Cyprinus carpio (Cole et al 1997); Pleuronectes americanus (LeMaitre et al 1997); Morone saxatilis´M. chrysops hybrid (Silphaduang & Noga 2001)). These species are members of different orders (Salmoniformes, Cypriniformes, Pleuronectiformes and Perciformes, respectively), suggesting that the presence of antimicrobial compounds in fish mucus is not phylogenetically constrained and such compounds may be ubiquitous among fishes.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial egg cleaning by the fringed darter (Perciformes: Percidae:Etheostoma crossopterum): implications of a novel component of parental care in fishes

Knouft

Page

Plewa

2003

Proc. R. Soc. Lond. B

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Broad-spectrum antimicrobial compounds have recently been identified in the epidermal mucus of fishes and probably serve as a first line of defence against microbial pathogens. Because of the ubiquitous nature of fungi and bacteria in aquatic systems, defence against these pathogens should be required throughout the lifespan of fishes, including the egg stage. We conducted experiments on Etheostoma crossopterum (Percidae: Catonotus), the fringed darter, to determine if the presence of a guarding male inhibits microbial colonization of eggs. Based on results from a combination of in-stream experiments, in vitro microbial assays, and morphological characteristics and behaviour of breeding males, we propose that antimicrobial egg cleaning by the guarding male is an effective component of parental care in these fish. Although innate antimicrobial compounds have been identified in a variety of organisms ranging from insects to vertebrates, integration of these compounds into a species's reproductive life history has been identified only in a small number of insect species. The results from this study not only indicate that E. crossopterum males provide a novel form of vertebrate parental care, but also have implications regarding the evolution of parental care in fishes and transitional evolutionary stages from no parental care to male parental care.

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“…Fish have evolved to thrive in an aqueous environment rich in microbial flora and are presumed to use their innate immune system as the first line of defense against microbial invasion. In the last decade, many species-specific antimicrobial peptides have been isolated from fish (misgurin, pleurocidin, paradaxins, hagfish intestinal antimicrobial peptides, and parasin I), some of them showed a broad spectrum of activity against Gram-positive and Gram-negative bacteria (Zasloff, 2002;Silphaduang & Noga, 2001). Compared with largely found antimicrobial peptides from aquatic animals, less antimicrobial proteins from aquatic species are reported.…”

Section: Discussionmentioning

confidence: 99%

“…Fish live in a microbe-rich environment and are vulnerable to be invaded by pathogenic or opportunistic micro-organisms. Compounds with broad antimicrobial activities are thought to be especially important for fish, as their adaptive immune system is structurally simpler than that of mammals or amphibians, and is not fully effective in young fry or at low environmental temperature (Silphaduang & Noga, 2001). The skin epithelium and other mucosal surfaces of fish are rich in anti-microbial peptides (Bergsson et al, 2005).…”

mentioning

confidence: 99%

Purification and Antimicrobial Activity of Antimicrobial Protein from Brown-spotted Grouper, <I>Epinephelus fario</I>

Zhang¹,

Zou²,

RiGa³

et al. 2009

Zoological Research

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Antimicrobial proteins and peptides had been found from a wide variety of organisms in the last few years. These molecules have attracted much research interest because of their biochemical diversity, broad specificity on anti-viral, anti-bacterial, anti-fungi, anti-protozoan parasites, anti-tumoural, and wound-healing effects. Antimicrobial proteins and peptides play key roles in innate immunity. They interact directly with bacteria and kill them. The brown-spotted grouper, Epinephelus fario, is an important marine fish cultured in southern China. Recently, bacteria and virus have caused high mortality in E. fario cultures, but its endogenous antimicrobial peptides and proteins have not been explored. An antimicrobial component was found from the skin homogenate of E. fario. After the skin homogenate was digested with trypsin, its antimicrobial activity was lost, which showed that the antimicrobial component is a protein. The antimicrobial protein (Efap) was purified from the skin homogenate of E. fario by successive ion-exchange and gel filtration chromatography. Efap was demonstrated to be single protein band by SDS-PAGE, with the apparent molecular weight of 41 kD. Efap exhibited antimicrobial activity both for the Gram-positive bacteria, Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis, and for the Gram-negative bacteria, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio fluvialis, Pasteurella multocida, Aeromonas hydrophila, Eschrrichiu coli, and Pseudomonas aeruginosa. Except A. hydrophila, P. aeruginosa, and E. coli (MIC>20 mol/L), most of the tested Gram-negative bacteria were sensitive to Efap (MIC<20 mol/L). Interestingly, Efap showed potent antimicrobial activity against Gram-positive bacteria S. aureus (MIC 5-10 mol/L) but comparatively weak antimicrobial activity against M. luteus and B. subtilis. The broad antimicrobial activities of Efap suggest that it contributes to the innate host defence of E. fario.

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Peptide antibiotics in mast cells of fish

Cited by 329 publications

References 11 publications

Discovery and characterization of two types of liver-expressed antimicrobial peptide 2 (LEAP-2) genes in rainbow trout

Discovery and characterization of two types of liver-expressed antimicrobial peptide 2 (LEAP-2) genes in rainbow trout

Antimicrobial egg cleaning by the fringed darter (Perciformes: Percidae:Etheostoma crossopterum): implications of a novel component of parental care in fishes

Purification and Antimicrobial Activity of Antimicrobial Protein from Brown-spotted Grouper, <I>Epinephelus fario</I>

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