Pentacyclic triterpenoids from the stem of Grewia bracteata Roth demonstrate promising inhibition on tumour cells

Abstract

Grewia bracteata Roth stem was investigated for its anticancer potential for the first time.Initially, polarity-guided extracts from three solvents were screened on HeLa, HCT-116 and MCF-7 tumours cells. The results revealed that ethyl acetate extract (GSE) significantly (p<0.05) inhibited HeLa, HCT-116 and MCF-7 cells with respective IC50 values of 30.58, 14.26 and 22.91 µg/ml. GSE inhibited HCT-116 cells with 6-and 21-folds higher than hexane (GSN) and methanol (GSM) extracts, respectively. Hence, column chromatography of GSE was performed and fractionated to 18 fractions. The obtained fractions were further tested on HCT-116 cells. Amongst, the fractions HF6 and DF1 were active with the respective IC50 values of 25.35 and 31.28, µg/ml (p<0.05). These active fractions were profiled using H 1 -NMR, C 13 -NMR and LC-MS/MS analysis, and found the presence of pentacyclic triterpenoids like betulin diacetate and ursolic acid.

Chemical Constituents From the Leaves of Grewia bulot Gagn. and Their Cytotoxic Activity

“…3 In addition, Grewia significantly contributed to the food processing sector, particularly in the productions of preserves, fruit juices, and teas. 4 It is found that plants of this genus served as a reservoir of diverse phytochemicals, 4 including flavonoids, 3,5 alkaloids, 3,6,7 triterpenoids, 3,8 phenols, 3,8,9 polysaccharides, 10,11 and others. 7,12–16 These compounds manifest a spectrum of bioactivities, such as antioxidant, anti-inflammatory, anticancer, antidiabetic, antibacterial, antispasmodic, antiviral, antimicrobial, and antimalarial properties.…”