Penta-<i>O</i>-galloyl-β-<scp>d</scp>-glucose Suppresses EGF-Induced eIF3i Expression through Inhibition of the PI3K/AKT/mTOR Pathway in Prostate Cancer Cells

Lin, Victor C.; Kuo, Po Tsun; Lin, Ying; Chen, Yeh; Hseu, You Cheng; Yang, Hsin Ling; Kao, Jung-Yie; Ho, Chi Tang; Way, Tzong‐Der

doi:10.1021/jf502447e

Cited by 19 publications

(12 citation statements)

References 30 publications

(56 reference statements)

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“…is result indicated that the MCF-7 breast cancer cell line treated with 60 μg/mL MPSE had elevated levels of ROS generation at 24 h treatment, in part due to the increase in the release of ROS from the damaged cells, a process so-called ROS-induced ROS release (RIRR) [42]. However, a further in-depth study focusing on the mitochondrial ROS generation, 16 Evidence-Based Complementary and Alternative Medicine determined by mitoSOX correlation with the effect of MPSE-treated breast cancer cells, must be performed to confirm this. Moreover, ROS produced in the mitochondria has been linked to mitochondrial membrane rupture and the loss of mitochondrial membrane potential (ΔΨm).…”

Section: Discussionmentioning

confidence: 99%

“…PGG has attracted attention because of its therapeutic potential and has shown certain functional properties such as antimicrobial, anti-inflammatory, anticancer, antidiabetic, and antioxidant activities [15]. PGG possesses antiproliferative effects on a variety of cancer cells including prostate cancer [16], liver cancer [17], and breast cancer [18].…”

Section: Introductionmentioning

confidence: 99%

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Pentagalloyl Glucose‐ and Ethyl Gallate‐Rich Extract from Maprang Seeds Induce Apoptosis in MCF‐7 Breast Cancer Cells through Mitochondria‐Mediated Pathway

Kantapan

Paksee²,

Chawapun

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Bouea macrophylla Griffith, locally known as maprang, has important economic value as a Thai fruit tree. The maprang seed extract (MPSE) has been shown to exhibit antibacterial and anticancer activities. However, the bioactive constituents in MPSE and the molecular mechanisms underlying these anticancer activities remain poorly understood. This study aims to identify the active compounds in MPSE and to investigate the mechanisms involved in MPSE-induced apoptosis in MCF-7 treated cancer cells. The cytotoxic effect was determined by MTT assay. The apoptosis induction of MPSE was evaluated in terms of ROS production, mitochondrial membrane potential depolarization, and apoptosis-related gene expression. The compounds identified by HPLC and LC/MS analysis were pentagalloyl glucose, ethyl gallate, and gallic acid. MPSE treatment decreased cell proliferation in MCF-7 cells, and MPSE was postulated to induce G2/M phase cell cycle arrest. MPSE was found to promote intracellular ROS production in MCF-7 treated cells and to also influence the depolarization of mitochondrial membrane potential. In addition, MPSE treatment can lead to increase in the Bax/Bcl-2 gene expression ratio, suggesting that MPSE-induced apoptosis is mitochondria-dependent pathway. Our results suggest that natural products obtained from maprang seeds have the potential to target the apoptosis pathway in breast cancer treatments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pentagalloyl Glucose‐ and Ethyl Gallate‐Rich Extract from Maprang Seeds Induce Apoptosis in MCF‐7 Breast Cancer Cells through Mitochondria‐Mediated Pathway

Kantapan

Paksee²,

Chawapun

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Phosphorylation of PI3K activates the phosphorylation of Akt and mTOR, which have central roles in the regulation of cell proliferation, growth, and survival [35]. Many previous studies have shown that natural substances have the ability to suppress the PI3K/Akt/mTOR signaling pathway in several cancer cells, thus, targeting the PI3K/Akt/mTOR signaling pathway is a key strategy for the suppression of cancer [36][37][38]. The levels of phospho-PI3K, phospho-Akt, and phospho-mTOR decreased in RC-58T cells following exposure to sanggenol L treatment ( Figure 6A).…”

Section: Discussionmentioning

confidence: 99%

Sanggenol L Induces Apoptosis and Cell Cycle Arrest via Activation of p53 and Suppression of PI3K/Akt/mTOR Signaling in Human Prostate Cancer Cells

Won

Seo

2020

Nutrients

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Prostate cancer is the most common cancer in Western countries. Recently, Asian countries are being affected by Western habits, which have had an important role in the rapid increase in cancer incidence. Sanggenol L (San L) is a natural flavonoid present in the root barks of Morus alba, which induces anti-cancer activities in ovarian cancer cells. However, the molecular and cellular mechanisms of the effects of sanggenol L on human prostate cancer cells have not been elucidated. In this study, we investigated whether sanggenol L exerts anti-cancer activity in human prostate cancer cells via apoptosis and cell cycle arrest. Sanggenol L induced caspase-dependent apoptosis (up-regulation of PARP and Bax or down-regulation of procaspase-3, -8, -9, Bid, and Bcl-2), induction of caspase-independent apoptosis (up-regulation of AIF and Endo G on cytosol), suppression of cell cycle (down-regulation of CDK1/2, CDK4, CDK6, cyclin D1, cyclin E, cyclin A, and cyclin B1 or up-regulation of p53 and p21), and inhibition of PI3K/Akt/mTOR signaling (down-regulation of PI3K, p-Akt, and p-mTOR) in prostate cancer cells. These results suggest the induction of apoptosis via suppression of PI3K/Akt/mTOR signaling and cell cycle arrest via activation of p53 in response to sanggenol L in prostate cancer cells.

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“…Poly-galloyl-glucopyranose or 1,2,3,4,6-penta-O-galloyl-βd-glucose (PGG), is a polyphenolic gallotannin synthesized by plants. Initially extracted from Rhus typhina (sumac) in 1990 by Hofmann and Gross, studies indicate that PGG has antidiabetic, antioxidant, anti-cancer and anti-inflammatory activities [26].…”

Section: Poly-galloyl-glucopyranosementioning

confidence: 99%

“…1, 2, 3, 4, and then incubated for 48 h. The induction pattern followed similar methods for this cancer cell line, as described in previous studies. We selected the PGG concentrations based on published literature describing PGG effects in several cell lines [26][27][28]31].…”

Section: Cell Culturementioning

confidence: 99%

Small molecule inhibition of matrix metalloproteinases as a potential therapeutic for metastatic activity in squamous cell carcinoma

et al. 2019

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Purpose One strategy for treating cancer is to prevent metastatic spread. Matrix metalloproteinase are considered potential targets for cancer therapy because of their role in degrading the extracellular matrix and fostering tumor progression. In some cancer models, the small molecule 1,2,3,4,6-Penta-O-galloyl-β-d-glucose (PGG) exhibited inhibitory properties against matrix metalloproteinase (MMP) related metastatic activity. This study explored whether PPG may limit the potential for metastatic spread in oral squamous cell carcinoma. Materials and methods This study used Cal-27 cells, a cell line derived from a squamous cell carcinoma line of human tongue origin, and antibodies for MMP-1,-2,-3,-9,-13, MT1-MMP signal transducers and activators of transcription 3 (Stat3), and pStat3. Cells were treated with PGG at different concentrations to evaluate MMP and Stat3 activation. Expansion assays were performed using Matrigel matrixes to measure Cal-27 invasiveness in the presence of PGG. Results PGG decreased the expression of MMP-2,-9 and-13 in the Cal-27 cell line, decreased phosphorylation of Stat3 and reduced gene expression of MMP-2,-9 and-13. As observed in Matrigel expansion assays, PGG limited the invasiveness of Cal-27 cells in a dose-dependent manner. Conclusion PPG is a small molecule inhibitor with the potential to reduce the expression of the matrix metalloproteinases and to limit the invasiveness of the squamous cell carcinoma line, Cal-27. By controlling the expression of molecules responsible for metastasis, PPG may offer a new therapeutic option for treating oral squamous cell carcinoma.

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Penta-O-galloyl-β-d-glucose Suppresses EGF-Induced eIF3i Expression through Inhibition of the PI3K/AKT/mTOR Pathway in Prostate Cancer Cells

Cited by 19 publications

References 30 publications

Pentagalloyl Glucose‐ and Ethyl Gallate‐Rich Extract from Maprang Seeds Induce Apoptosis in MCF‐7 Breast Cancer Cells through Mitochondria‐Mediated Pathway

Pentagalloyl Glucose‐ and Ethyl Gallate‐Rich Extract from Maprang Seeds Induce Apoptosis in MCF‐7 Breast Cancer Cells through Mitochondria‐Mediated Pathway

Sanggenol L Induces Apoptosis and Cell Cycle Arrest via Activation of p53 and Suppression of PI3K/Akt/mTOR Signaling in Human Prostate Cancer Cells

Small molecule inhibition of matrix metalloproteinases as a potential therapeutic for metastatic activity in squamous cell carcinoma

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