PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

Hartati, Sri; Budiyono, Agus; Cahyono, Ongko

doi:10.20961/carakatani.v31i1.11938

Cited by 13 publications

(14 citation statements)

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“…Keadaan ini mengindikasikan bahwa sito-kinin dan auksin endogen mencukupi untuk pertumbuhan protokorm menjadi planlet. Hasil yang sama terdapat pada percobaan Hartati et al, (2016), bahwa tidak terjadi interaksi antara BAP dan NAA terhadap semua variabel pengamatan (jumlah tunas, panjang tunas, panjang akar, dan jumlah akar).…”

Section: Hasil Dan Pembahasanunclassified

Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

et al. 2019

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The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium spectabile orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1. The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves. Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets. BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture

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Section: Hasil Dan Pembahasanunclassified

Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

et al. 2019

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“…Application of excessive auxin to the culture media will cause an imbalance in the interaction with endogenous auxin and cannot produce a greater number of roots [17]. Similarly, administration of high concentrations of auxin found to affects the formation of many plant roots but can inhibit root elongation [18]. Higher administration of exogenous cytokinin hormones can have an impact on inhibition of shoot formation [19] and in turn causes stunted growth in plants [8].…”

Section: Discussionmentioning

confidence: 99%

In vitro multiplication of “Kepok” banana (Musa acuminata x Musa balbisiana) using different concentration of BAP and NAA in MS media

Safitri,

Ridwan,

Yassi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study aims to determine the effect of different composition of BAP and NAA concentration added to Murashige and Skoog (MS) basic media on the in vitro multiplication of Indonesian “Kepok” banana variety. The research was conducted at the Tissue Culture Laboratory of Bonto-Bonto Horticultural Plant Seed Center. A completely randomized design (CRD) was employed with two factors tested. Four concentrations of each BAP and NAA used were 0 ppm, 4.5 ppm, 5 ppm, and 5.5 ppm for BAP, and 0 ppm, 2 ppm, 2.5 ppm, and 3 ppm for NAA resulted in 15 composition of BAP:NAA in addition to control (without the hormones). The results show no interaction between the BAP and NAA treatments, BAP concentration of 5 ppm gave the best result on parameter time to shoots emergence (7.50 DAP). NAA concentration of 2.5 ppm gave the best results on root emergence time (8.61 DAP), time to plantlet formation (11.72 DAP), number of roots (4.81 roots) and root length (5.93 cm).

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“…Hal ini diduga bahwa konsentrasi ZPT eksogen yang ditambah pada eksplan M petola terlalu tinggi sehingga menghambat pertumbuhan eksplan. Menurut Hartati dkk [14], tinggi pada tanaman merupakan ukuran pertumbuhan yang paling mudah untuk dilihat. Adanya pertambahan tinggi eksplan dapat disebabkan oleh dua proses yaitu pembelahan dan juga pemanjangan sel.…”

Section: Tinggi Batangunclassified

The Effect of Various Concentration BAP (6-Benzyl Amino Purine) on Orchid Growth (Macodes petola (Blume) Lindl.) In-Vitro

et al. 2022

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Macodes petola (Blume) Lindl. has an attractive of leaves veins motif with letters pattern, so it has a high economic value. Tissue culture technique is reported as the best way of plant propagation in short time. BAP (6-Benzyl Amino Purine) has a role in stimulating shoot growth with a certain concentration. The purpose of this study was to determine the effect of Various Concentration BAP (6-Benzyl Amino Purine) on Orchid Growth (M petola (Blume) Lindl.) in vitro. This study used a completely randomized design (CRD) with 4 concentration BAP levels (0; 0.5; 1; 1.5 ppm) with 3 replication. Based on the results of the study, it showed that up to 60 DAP (Day After Planting) the composition of the media with a concentration of 0 ppm BAP had a higher average value for the increase in the stem height, number of shoots and number of roots, namely 1.77±0.798 cm, 2.4 ± 1,528 shoot and 2.4 ± 2,082 root. In contrast to the concentration of 1.5 ppm at this concentration it has a low average value the parameters of stem height, number of shoots and number of roots.

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PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

Cited by 13 publications

References 0 publications

Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

In vitro multiplication of “Kepok” banana (Musa acuminata x Musa balbisiana) using different concentration of BAP and NAA in MS media

The Effect of Various Concentration BAP (6-Benzyl Amino Purine) on Orchid Growth (Macodes petola (Blume) Lindl.) In-Vitro

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