PENGARUH KONDISI KULTUR PADA AKTIVITAS SELULASE ISOLAT Pycnoporus sp. DAN Phlebiopsis sp. (EFFECTS OF CULTURE CONDITIONS ON CELLULASE ACTIVITIES PRODUCED BY Pycnoporus sp. AND Phlebiopsis sp.)

Abstract

ABSTRAKPenelitian pengaruh media kultivasi, pH dan suhu inkubasi terhadap produksi enzim selulase-kompleks dari Pycnoporus sp. FORDACC-03452 dan Phlebiopsis sp. FORDACC-02482 yang ditumbuhkan di media dedak padi dan tongkol jagung dengan metode kultur padat pada variasi pH 4-7 dan suhu 30°C-45°C, telah dilakukan. Hasil memperlihatkan bahwa media dedak padi cenderung menginduksi produksi endo-β-1,4-glukanase dan selobiohidrolase, sedangkan media tongkol jagung menginduksi produksi β-glukosidase. Namun, campuran kedua substrat tersebut tidak menghasilkan aktivitas selulase yang lebih baik. Selulase-kompleks yang dihasilkan Pycnoporus sp. menunjukkan aktivitas lebih baik dibandingkan dengan yang diproduksi Phlebiopsis sp. Filtrat kasar Pycnoporus sp. menunjukkan aktivitas-spesifik endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,403 ± 0,010 IU/mg); selobiohidrolase pada pH 6, suhu 40°C (0,540 ± 0,020 IU/mg); dan β -glukosidase pada pH 4, suhu 30°C (0,022±0,001 IU/mg). Sementara, Phlebiopsis sp. menunjukkan aktivitas-spesifik endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,202 ± 0,005 IU/mg); selobiohidrolase pada pH 4, suhu 45°C (0,180 ± 0,002 IU/mg); dan β-glukosidase pada pH 6, suhu 45°C (0,007 ± 0,001 IU/mg). Rendahnya aktivitas β-glukosidase menyebabkan selulase-kompleks dari penelitian ini belum dapat menghidrolisis limbah lignoselulosa dengan sempurna dan kadar glukosa yang diperoleh masih rendah. Oleh karena itu, optimasi produksi selulasekompleks dari Pycnoporus sp. dan Phlebiopsis sp. masih perlu diteliti lebih lanjut.

Biological Degradation of Bamboo Paper by Two White-Rot Fungi Species

“…and Phlebiopsis sp. are some of the white rot fungi that have been studied for their cellulase activity, which demonstrated that the Pycnoporus isolate produces cellulase with higher activity than the Phlebiopsis isolate (Agustini et al, 2017).…”

“…Pycnoporus sp. and Phlebiopsis sp., whose cellulolytic activities have been characterized (Agustini et al, 2017), can be tested for this purpose. Moreover, a study reported that the presence of carbonaceous products stimulated the proliferation of soilresident fungi and enhanced the enzymatic activities (Taskin, Teresa, Altomare, & Loffredo, 2019).…”

Coffee Pulp Activated Carbon for Immobilizing Cellulase from Aspergillus niger ICP2: Enhancing Enzyme Stability, Activity, and Its Reusability

“…The absorbance value of the supernatant was measured with a spectrophotometer at a wavelength of 500 nm. Cellulase activity was calculated based on the reducing sugar value obtained using the formula in equation (1).…”

“…Cellulase is an enzyme complex capable of breaking down cellulose into glucose monomers. This complex comprises endocellulase, exocellulase, and β-glucosidase, which, in microorganisms, work synergistically to break down cellulose polymer chains [1,2]. Cellulase has been widely utilized in various fields, including textile, animal feed, agriculture, pharmaceutical, detergent, bioconversion, paper, and pulp industries [3,4].…”