Pengaruh Ekstrak Etanol Propolis Terhadap Aktivitas dan Kapasitas Fagositosis pada Kultur Makrofag yang Diinfeksi Enteropathogenic Escherichia coli (EPEC)

Abstract

AbstrakPenanganan penyakit infeksi diperlukan suatu imunostimulator. Propolis adalah campuran resin yang dikumpulkan lebah dari tumbuh-tumbuhan, digunakan sebagai material isolasi sarang lebah, merupakan bahan yang berpotensi sebagai imunostimulator. Penelitian dilakukan di Laboratorium Penelitian dan Pengujian Terpadu Universitas Gadjah Mada pada Januari-Maret 2013. Penelitian ini bertujuan menganalisis peningkatan aktivitas dan kapasitas fagositosis kultur makrofag yang diberi ekstrak etanol propolis terhadap Enteropathogenic Escherichia coli (EPEC). Desain penelitian eskperimen. Ke dalam kultur makrofag yang diperoleh dari tiga orang subjek, masing-masing diberi ekstrak etanol propolis 6,25; 12,5; 25; 50, dan 100 µg/mL serta kontrol tanpa ekstrak etanol propolis. Inkubasi dilakukan satu hari. Kultur diinfeksikan EPEC selama 30 menit, diwarnai dengan Giemsa dan diamati dengan mikroskop. Persentase aktivitas fagositosis diperoleh dengan menghitung jumlah makrofag yang aktif dalam 100 makrofag. Nilai kapasitas fagositosis diperoleh dengan menghitung jumlah bakteri yang difagosit oleh 50 makrofag. Uji analysis of variance (ANOVA) menunjukkan perbedaan signifikan aktivitas maupun kapasitas fagositosis kultur makrofag yang diberi ekstrak etanol propolis dibanding dengan kontrol (p=0,000). Uji Tukey HSD pada aktivitas fagositosis menunjukkan hasil signifikan antara kontrol dan konsentrasi 6,25 µg/mL (p=0,008), sedangkan pada kapasitas fagositosis terlihat antara kontrol dan konsentrasi 12,5 µg/mL (p=0,001). Simpulan, ekstrak etanol propolis meningkatkan aktivitas dan kapasitas fagositosis kultur makrofag terhadap EPEC dengan konsentrasi minimum 12,5 µg/mL. Effect of Ethanolic Extract Propolis on Phagocytosis Activity and Capacity in Macrophages Culture Infected by Enteropathogenic Escherichia coli (EPEC) AbstractThe use of immunostimulant for infectious diseases treatment is needed. Propolis is a mixture of resin collected from plants by bees and used as an insulating material in beehives which has a potential as immunostimulant. This study was performed at Laboratory of Research and Integrated Testing, Gadjah Mada University, in January− March 2013. This experimental research aimed to analyze the increase of phagocytosis activity and capacity on macrophages culture added by propolis ethanolic extract against Enteropathogenic Escherichia coli (EPEC). The design of this study was experimental study. Macrophage cultures obtained from three subjects were individually culture supplemented with the 6.25, 12.5, 25, 50, and 100 µg/mL propolis ethanolic extract. A control without the addition of propolis ethanolic extract is also used. Cultures were incubated for one day. Macrophage cultures were infected by EPEC for 30 minutes, stained by Giemsa, and observed by microscope. Percentage of phagocytosis activity is determined by the number of active macrophages per 100 macrophages. The phagocytosis capacity value was determined by counting the number of bacteria that were phagocytized by 50 macrophages. Analysis of variance ...

Effect of Lactic Acid Filtrate and Bacteriocins of Lactobacillus acidophillus on Phagocytosis Activity of Macrophages Cell againts Enteropathogen Escherichia coli (EPEC)

“…Isolated PBMC transferred into new falcon tube and washed by HBSS (1:1). Macrophages culture was performed according to Herawati et al (2013) briefly, two hundreds microliter of isolated PBMC was dispensed into each well of multidish 24 wells which contains coverslip, and incubated for two hours at 37 o C and 5% CO . Culture supernatants from every well 2 were discarded and washed by HBSS followed by giving 500 µL of complete RPMI (Roswell Park Memorial Institute), incubation was then continued to o 7-10 d at 37 C and 5% Co .…”

“…Phagocytosis Activity Examination. Macrophages phagocytosis activity examination againts EPEC was performed in duplicate according to Chairul et al (2009) and Herawati et al (2013). Briefly, 500 µL of lactic acid filtrate or bacteriosins from various concentration dispensed into each well of culture containing mature macrophages followed by adding RPMI to get the final indicated concentration of lactic acid filtrate or bacteriosin in -1 each well 12.5, 6.25, and 3.125 µg mL , including control without filtrate addition.…”

Tissue repair in post extraction sockets of Cavia cobaya induced by a combination of propolis and graft

“…Analysis of the data on day 3 confirmed that the group induced with propolis and those induced with BBG and propolis presented significant differences compared to the control group. This is due to the fact that propolis can increase TLR-2 expression within which TLR activates macrophages with the result that when TLR expression increases the amount of macrophages also rises (Herawati et al, 2015). Moreover, propolis can improve the function of macrophage phagocytes and also enhance the release of interferon-gamma (IFN-γ), interleukin-6 (IL-6), and interleukin-1β (IL-1β) (Hariri, 2019).…”

EFEK SELENIUM OKSIKLORIDA TERHADAP AKTIVITAS IMUNOMODULATOR DARI EKSOPOLISAKARIDA Lactobacillus plantarum

“…Bagian perut dibersihkan dengan menggunakan etanol 70% dan dibedah diatas papan bedah. Cairan peritoneum dibuat preparat ulas pada gelas objek dan difiksasi dengan metanol kemudian dilakukan pewarnaan dengan Giemsa (Herawati et al, 2015). Pengamatan dilakukan di bawah mikroskop dengan perbesaran 10x100 kali.…”