Pengaruh Cekaman Aluminium terhadap Kandungan Asam Organik dalam Kalus dan Pinak Tomat (Lycopersicon esculentum Mill.)

Abstract

The Effects of Aluminum Stress on Organic Acid Content of Lycopersicon esculentum Mill. cv. Intan Callus and Plantlet. Wening Enggarini and Erly Marwani. The purpose of this research was to evaluate the effects of Al stress on citric, malic and oxalic acid content of L. esculentum cv. Intan callus and plantlet, also aluminum content of L. esculentum plantlet. Callus was induced from cotyledone of L. esculentum on Murashige & Skoog (MS) media containing 10 -7 M NAA and 10 -6 kinetin. The callus was then transferred step wisely at 3 weeks interval to media containing 220, 275, 330, 385, 440, 550, 825, and 1100 μM AlCl 3 . The callus cultures on the control media and media with the addition of 550 μM AlCl 3 were able to regenerate and produce shoots after 8 passages of subculture. The shoots from media with the addition of 550 μM AlCl 3 were transferred into the media with addition of 825 μM AlCl 3 , then to the media with 1100 μM AlCl 3 . The High Pressure Liquid Chromatography (HPLC) analysis showed that Al stress callus and plantlets contained malic acid, but no citric and oxalic acid. The content of malic acid in callus decreased with increasing AlCl 3 concentration from 0 to 385 μM. On the other hand, the content of malic acid in callus increased with increasing AlCl 3 concentration from 440 μM to 1100 μM. Similarly, the content of malic acid in root increased with increasing concentration of AlCl 3 from 550 μM to 1100 μM. The result of Neutron Activation Analysis showed that Al content in root decreased as the amount of AlCl 3 increased in the media. These results suggested that L. esculentum callus and plantlet respond to the Al stress by producing higher amount of malic acid.

Proline Activity and Growth of Oil Palm affected by Aluminium Toxicity and Silica as Ameliorant

“…Forforilase barriers cause ATP to form, yet hexaphospate such as glucose-6phosphate and ADP formation would be disrupted. According to Enggarini and Marwani (2006), phosphorylation disruption leads to leaf chlorosis, nutrient deficiency, and stunt.…”