Penentuan Serotipe Virus Dengue dan Gambaran Manifestasi Klinis serta Hematologi Rutin pada Infeksi Virus Dengue

Abstract

AbstrakSetiap serotipe virus dengue dapat menimbulkan manifestasi klinis mulai dari demam dengue (DD) sampai demam berdarah dengue (DBD) dan sindrom syok dengue (SSD). Sulit untuk menilai karakteristik klinis dan hasil hematologi yang khas untuk setiap serotipe. Tujuan penelitian ini untuk menentukan serotipe virus dengue dan gambaran manifestasi klinis DD, DBD, SSD serta hasil pemeriksaan hematologi rutin yaitu hemoglobin, hematokrit, leukosit, dan trombosit pada setiap serotipe virus dengue. Penelitian dilakukan di Rumah Sakit Dr. Hasan Sadikin Bandung sejak Maret 2010 sampai Juli 2011. Subjek penelitian adalah penderita infeksi dengue usia >14 tahun dengan riwayat demam <5 hari. Sampel darah diambil untuk penentuan serotipe dengue dengan teknik reverse transcription polymerase chain reaction (RT-PCR) dilanjutkan dengan seminested PCR. Data manifestasi klinis dan hematologi rutin diperoleh dari penelusuran rekam medis. Penelitian ini merupakan penelitian deskriptif. Sebanyak 75 penderita ikut dalam penelitian. Serotipe virus dapat dideteksi pada 27 (36%) sampel dengan dominasi DENV-3 (13) diikuti DENV-2 (8), DENV-4 (4), dan DENV-1 (2). Kasus DBD ditemukan paling banyak pada infeksi DENV-3. Infeksi DENV-2 memberikan penurunan hemoglobin tertinggi, persentase peningkatan hematokrit tertinggi, leukosit terendah, dan trombosit terendah dibandingkan dengan serotipe lainnya. Dengue Virus Serotyping and Its Clinical Manifestation and Routine Haematology in Dengue Infections AbstractAll DENV serotypes can cause a spectrum of disease from dengue fever (DF) to dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It is difficult to differentiate clinical characteristicand hematologic result for each serotype. Aim of this study were to determine dengue serotype and describe clinical manifestation of DF, DHF, DSS and routine hematologic results, i.e.haemoglobin, hematocrit, leukocyte, and thrombocyte in each serotype. This study was conducted at Dr. Hasan Sadikin Hospital Bandung from March 2010 until July 2011. Subjects were dengue patients aged >14 years with a history of fever <5 days. Blood samples were taken for serotype determination by reverse transcription polymerase chain reaction (RT-PCR) followed by semi-nested PCR. Clinical manifestation data and haematologic result were obtained from medical records. This was a descriptive study. Seventy five patients were included in this study. Dengue serotype can be detected in 27 (36%) samples with DENV-3 (13) were dominating followed by DENV-2 (8), DENV-4 (4), and DENV-1 (2). DHF was mainly found in DENV-3. DENV-2 gavethe highest decrease in hemoglobin, highest percentage increase in haematocrit, lowest leukocyte, and lowest thrombocyte. In conclusion, all 4 serotypes are found in RSUP Dr. Hasan Sadikin Hospital Bandung with DENV-3 domination. DHF is mainly caused by DENV-3. In routine hematology, the most abnormal results are found in DENV-2 infection. [MKB. 2012;44(4):253-60].

Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades

“…A full assessment of the 120 references resulted in the exclusion of 53 references due to the following: full texts were unavailable, and the available abstracts did not include adequate information to satisfy our outcome, the full text did not include adequate or complete serotype data, and nonstandard methods were used for serotyping. This resulted in 67 eligible studies, of which 13 references were from national journals . An additional 11 studies were included from reference list .…”

“…In total, 78 eligible studies were included for DENV serotype analysis . Of all the eligible studies, four studies were conducted based on mosquito isolates while the remaining studies assessed DENV isolates from human samples . Sixty two studies investigated dengue cases among local inhabitants of Indonesia,…”

“…Between 2008 and 2012, however, DENV‐1 become the dominant serotype in most Indonesian regions (Table ). Data of traveller‐associated dengue from Australia, Taiwan, Germany, and Romania support this finding.…”

Correlation of Dengue Virus Serotype and Dvi Severity in Adult Patients

“…2 Patients who have been exposed to different dengue virus serotypes will have risk factors for severe DVI manifestations or DSS. 3,4 Serological test is usually performed to detect antigens in the form of NSI3, while anti-dengue IgM and IgG tests are conducted to detect IgM and IgG antibodies. 4,5 The method commonly used in those examinations is immunochromatography.…”

“…3,4 Serological test is usually performed to detect antigens in the form of NSI3, while anti-dengue IgM and IgG tests are conducted to detect IgM and IgG antibodies. 4,5 The method commonly used in those examinations is immunochromatography. Nevertheless, although the serological test is useful for the detection of DVI from day 2-3 to 7 after the fever, it is difficult to detect specifically at the onset of fever (≤ five days).…”