Pemphigus vulgaris. Superior sensitivity of monkey esophagus in the determination of pemphigus antibody

Feibelman, Cary E.

doi:10.1001/archderm.117.9.561

Cited by 25 publications

(18 citation statements)

References 8 publications

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“…Total IgG concentration was measured by nephelometry using monospecific goat anti-human IgG (Beckman Instruments, Missasauga, Ontario, Canada). Pemphigus Ab titers were measured by indirect immunofluorescence using monkey esophagus epithelium as the tissue substrate (22). As a negative control, IgG fractions were isolated and removed from PV plasma using protein A (PA).…”

Section: Preparation Of Pemphigus Iggmentioning

confidence: 99%

“…Sera were collected 24 h after PV IgG treatment, PA treatment (IgG depleted), or sham treatment. Indirect immunofluorescence studies were performed according to standard techniques (22,26). Cryostat sections (5 m) of monkey esophagus were employed as substrate, washed for 10 min in PBS, incubated for 30 min with different concentrations of sera (1:1-1:600), washed in PBS for 15 min, labeled with FITC-conjugated F(abЈ) 2 fragment of rabbit anti human IgG (Dako) for 30 min, and then washed again in PBS for 15 min.…”

Section: Tissue Specimens Stainingmentioning

confidence: 99%

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Immune Modulation in Pemphigus Vulgaris: Role of CD28 and IL-10

Toto

Feliciani

Amerio

et al. 2000

The Journal of Immunology

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Pemphigus vulgaris (PV) is an autoimmune bullous skin disease characterized by Abs to the desmosomal cadherin desmoglein-3. Although the autoantibodies have been shown to be pathogenic, the role of the cellular immune system in the pathology of pemphigus-induced acantholysis is unclear. To further delineate the potential role of T cell-signaling pathways in the pathogenesis of PV, we performed passive transfer experiments with PV IgG in gene-targeted mutant mice. Our results demonstrated that CD28-deficient mice (lacking a costimulatory signal for T cell activation) are 5-fold more sensitive to the development of PV than wild-type mice. To evaluate whether the higher incidence of disease was due to an impairment in intercellular adhesion of keratinocytes, we performed an in vitro acantholysis, using CD28−/− mice keratinocytes. No alteration in in vitro adhesion was detected in CD28−/−-type keratinocytes. Because the CD28 molecule plays a pivotal role in the induction of Th2 cytokines, we examined the levels of a prototypic Th2 cytokine (IL-10) in CD28−/− mice. Lower levels of IL-10 mRNA were found in lesions from CD28−/− mice. To determine whether pemphigus susceptibility in CD28−/− was related to IL-10 deficiency, we performed passive transfer experiments in IL-10−/− mice that demonstrated increased blisters compared with controls. To confirm that IL-10 is involved in the pathogenesis, rIL-10 was given with PV IgG. IL-10 significantly suppressed the disease activity. These data suggest a potential role of IL-10 in PV.

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Section: Preparation Of Pemphigus Iggmentioning

confidence: 99%

Section: Tissue Specimens Stainingmentioning

confidence: 99%

Immune Modulation in Pemphigus Vulgaris: Role of CD28 and IL-10

Toto

Feliciani

Amerio

et al. 2000

The Journal of Immunology

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“…Serum from one PV patient was then diluted in PBS over a range of eight doubling dilutions from 1:10 to 1:1280 [11]. The final titer that was obtained from ME substrate was used as a reference titer for this serum.…”

Section: Methodsmentioning

confidence: 99%

“…For IIF technique, patient serum is used to identify circulating IgG antibodies to the intercellular spaces of keratinocytes of substrate. Monkey esophagus (ME) and guinea pig esophagus are well-accepted substrates for pemphigus vulgaris (PV) and pemphigus foliaceous (PF), respectively [11,14]. Sensitivity of IIF on ME ranged from 81 to 100 % [2,14,[32][33][34]37].…”

Section: Introductionmentioning

confidence: 99%

Human cervix: an alternative substrate for detecting circulating pemphigus antibodies

et al. 2016

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Monkey esophagus (ME) is a well-accepted substrate for diagnosing pemphigus vulgaris (PV) by indirect immunofluorescence (IIF). However, its availability is sometimes limited due to ethical concerns. This study aimed to investigate the usefulness of human cervix (HC) as a substrate in the diagnosis of PV by IIF. Initially, serum from 1 PV patient was incubated with tissues from 48 HCs. Median IIF titers on HCs that had different demographic and clinical characteristics were compared. Sera from 5 PV patients were then incubated with ME and 21 HCs. For each serum, the titer of IIF on HC that was not different from ME by more than two-fold dilutions was acceptable. Last, sera from 42 PV, 14 pemphigus foliaceous, and 62 non-pemphigus patients were used to evaluate sensitivity and specificity. The results demonstrate that differences in demographic data among HCs did not affect IIF titers. Titers obtained from ME and HC were comparable (81-100 % acceptable values). Sensitivity of HC for diagnosis of PV was better than for diagnosis of pemphigus foliaceus (90.5 and 71.4 %, respectively). Specificity for PV and PF was 96.2 %. We proposed that HC substrate can be used as an alternative substrate for diagnosis of PV by IIF.

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“…Total IgG concentration was measured by nephelometry using monospecific goat anti-human IgG (Beckman Instruments, Missasauga, ON, Canada). Pemphigus Ab titers were measured by indirect immunofluorescence (IIF) using monkey esophagus epithelium as the tissue substrate [13]. As a negative control, IgG fractions were isolated and removed from PV plasma using protein A (PA).…”

Section: Methodsmentioning

confidence: 99%

A Possible Role for CD8+ T Lymphocytes in the Cell-Mediated Pathogenesis of Pemphigus Vulgaris

Giurdanella

Fania

Gnarra

et al. 2013

Mediators of Inflammation

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Pemphigus vulgaris (PV) is an autoimmune blistering disease whose pathogenesis involves both humoral and cell-mediated immune response. Though the pathogenetic role of autoantibodies directed against desmoglein 3 is certain, a number of other factors have been suggested to determine acantholysis in PV. In this study we examined the possible role of CD8+ T cells in the development of acantholysis by a passive transfer of PV autoantibodies using CD8 deficient mice, and we also studied the inflammatory infiltrate of PV skin lesions by immunohistochemical staining. The results of the immunohistochemical staining to study the expression of CD3, CD4, and CD8 in PV skin lesions showed that CD4+ are more expressed than CD8+ in the inflammatory infiltrate of PV lesions, confirming the data of the previous literature. The passive transfer study showed a lower incidence of pemphigus in the group of CD8 deficient mice compared to the control one of wild-type mice. These results suggest that CD8+ T cells may play a role in the pathogenesis of PV, perhaps through the Fas/FasL pathway.

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Pemphigus vulgaris. Superior sensitivity of monkey esophagus in the determination of pemphigus antibody

Cited by 25 publications

References 8 publications

Immune Modulation in Pemphigus Vulgaris: Role of CD28 and IL-10

Immune Modulation in Pemphigus Vulgaris: Role of CD28 and IL-10

Human cervix: an alternative substrate for detecting circulating pemphigus antibodies

A Possible Role for CD8+ T Lymphocytes in the Cell-Mediated Pathogenesis of Pemphigus Vulgaris

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