PEI-alginate nanocomposites as efficient in vitro gene transfection agents

Patnaik, Satyakam; Aggarwal, Anita; Nimesh, Surendra; Goel, Anita; Ganguli, Munia; Saini, Neeru; Singh, Yogendra; Gupta, Kailash C.

doi:10.1016/j.jconrel.2006.06.025

Cited by 89 publications

(52 citation statements)

References 49 publications

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“…Furthermore, the low transfection efficiency of Ca-pectinate nanoparticles might be due to the larger particle size of Capectinate and the negative charge of those particles. Therefore, in future experiments, a cationic polymer such as polyethyleneimine (PEI) (30), poly-L-lysine (PLL) (21), or chitosan might be added to enhance the positive charge and the DNA loading efficiency, which might improve the DNA entrapment and transfection efficiency.…”

Section: In Vitro Gene Expressionmentioning

confidence: 99%

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

et al. 2008

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Abstract. The aim of this study was to investigate the possibility of using pectinate micro/nanoparticles as gene delivery systems. Pectinate micro/nanoparticles were produced by ionotropic gelation. Various factors were studied for their effects on the preparation of pectinate micro/nanoparticles: the pH of the pectin solution, the ratio of pectin to the cation, the concentration of pectin and the cation, and the type of cation (calcium ions, magnesium ions and manganese ions). After the preparation, the size and charge of the pectin micro/nanoparticles and their DNA incorporation efficiency were evaluated. The results showed that the particle sizes decreased with the decreased concentrations of pectin and cation. The type of cations affected the particle size. Sizes of calcium pectinate particles were larger than those of magnesium pectinate and manganese pectinate particles. The DNA loading efficiency showed that Capectinate nanoparticles could entrap DNA up to 0.05 mg when the weight ratio of pectin:CaCl 2 :DNA was 0.2:1:0.05. However, Mg-pectinate could entrap only 0.01 mg DNA when the weight ratio of pectin: MgCl 2 :DNA was 1:100:0.01 The transfection efficiency of both Ca-pectinate and Mg-pectinate nanoparticles yielded relatively low levels of green fluorescent protein expression and low cytotoxicity in Huh7 cells. Given the negligible cytotoxic effects, these pectinate micro/nanoparticles can be considered as potential candidates for use as safe gene delivery carriers.

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Section: In Vitro Gene Expressionmentioning

confidence: 99%

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

et al. 2008

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“…it is important to refer that the toxicity of PEI should not be a problem because it was used as the deepest layer which remained attached to the surface. Moreover, there are several reports in the literature about the reduction of the cytotoxicity of PEI in presence of chitosan and alginate [49,50]. However, cytocompatibility tests would be necessary before in vivo application.…”

Section: Optimization Of the Lbl Coatingmentioning

confidence: 99%

Chitosan/alginate based multilayers to control drug release from ophthalmic lens

Silva

Pinto²,

Bozukova

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…In order to produce highly efficient PEI-al nanocomposites, alginate and PEI were fabricated using a procedure developed by Patnaik et al 22 …”

Section: Synthesis Of Pei-al Nanocomposites and Pei-al/dna Complexesmentioning

confidence: 99%

“…22,23 Different types of PEI are regarded as the most effective nonviral gene vectors, due to their ability to reduce the negative charge of DNA and partially condense its normally large hydrodynamic volume and the ability to escape from lysosomes. 5,25 However, PEI exhibits high cytotoxicity, so many efforts have been made to modify PEI in order to decrease its cytotoxicity whilst retaining the high transfection efficiency.…”

Section: Weeks (×20) (B) Blank Control Group After 8 Weeks (×20) (Cmentioning

confidence: 99%

“…However, the use of nonviral gene vectors has emerged as a viable alternative to viral-based gene vectors, because they overcome the limitation of viral vectors, such as immune response, oncogenicity, and difficulty in large-scale production and purification. 4,21 Patnaik et al 22 prepared a series of polyethylenimine (PEI) (linear 750 kDa and branched 25 kDa)-alginate (PEI-al) nanocomposites as a kind of novel nonviral gene vector. PEI-al nanocomposites exhibit higher transfection efficiency and lower cytotoxicity than those of commercial transfection reagents.…”

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confidence: 99%

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Efficiently engineered cell sheet using a complex of polyethylenimine–alginate nanocomposites plus bone morphogenetic protein 2 gene to promote new bone formation

Jin¹,

Zhang²,

Qiao³

et al. 2014

IJN

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Regeneration of large bone defects is a common clinical problem. Recently, stem cell sheet has been an emerging strategy in bone tissue engineering. To enhance the osteogenic potential of stem cell sheet, we fabricated bone morphogenetic protein 2 ( BMP-2 ) gene-engineered cell sheet using a complex of polyethylenimine–alginate (PEI–al) nanocomposites plus human BMP-2 complementary(c)DNA plasmid, and studied its osteogenesis in vitro and in vivo. PEI–al nanocomposites carrying BMP-2 gene could efficiently transfect bone marrow mesenchymal stem cells. The cell sheet was made by culturing the cells in medium containing vitamin C for 10 days. Assays on the cell culture showed that the genetically engineered cells released the BMP-2 for at least 14 days. The expression of osteogenesis-related gene was increased, which demonstrated that released BMP-2 could effectively induce the cell sheet osteogenic differentiation in vitro. To further test the osteogenic potential of the cell sheet in vivo, enhanced green fluorescent protein or BMP-2-producing cell sheets were treated on the cranial bone defects. The results indicated that the BMP-2-producing cell sheet group was more efficient than other groups in promoting bone formation in the defect area. Our results suggested that PEI–al nanocomposites efficiently deliver the BMP-2 gene to bone marrow mesenchymal stem cells and that BMP-2 gene-engineered cell sheet is an effective way for promoting bone regeneration.

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PEI-alginate nanocomposites as efficient in vitro gene transfection agents

Cited by 89 publications

References 49 publications

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

Chitosan/alginate based multilayers to control drug release from ophthalmic lens

Efficiently engineered cell sheet using a complex of polyethylenimine–alginate nanocomposites plus bone morphogenetic protein 2 gene to promote new bone formation

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PEI-alginate nanocomposites as efficient in vitro gene transfection agents

Cited by 89 publications

References 49 publications

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

Chitosan/alginate based multilayers to control drug release from ophthalmic lens

Efficiently engineered cell sheet using a complex of polyethylenimine&ndash;alginate nanocomposites plus bone morphogenetic protein 2 gene to promote new bone formation

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Efficiently engineered cell sheet using a complex of polyethylenimine–alginate nanocomposites plus bone morphogenetic protein 2 gene to promote new bone formation