“…The IAA levels produced by P. chlororaphis O6 with the various treatments (5 to 37 g/ml) were in the region that would boost rather than inhibit growth (3,4,24,32). The levels of Cu and Zn from the NPs used in testing the soil bacterium were relevant to levels reported for contaminated soils, ranging from 100 mg/kg to 1,000 mg/kg of Cu and 2,000 mg/kg of Zn; normal soils contain between 22 and 68 mg/kg Cu and 92 and 180 mg/kg Zn (14,33,38,39,41). At 200 mg of Cu/liter, the CuO NPs increased the conversion of trp to IAM in 15-h cultures and resulted in higher IAA levels in the culture medium by 48 h than in control cultures.…”