Peculiarities of calcium and iron effects on some wild terrestrial orchids in vitro compared to in vivo

Jakobsone, Gunta; Osvalde, Anita

doi:10.1007/s11627-019-09959-5

Cited by 2 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This explanation may be suitable for the G. conopsea low germination rates obtained in our study, as well as in [11], where germination was also poor (20%). Various studies reported that the traditional tissue culture media have been found to be too concentrated for temperate orchids, therefore, should be diluted approximately 2 to 10 times [14]. In our study, the m1/3 MS medium that worked well for seed germination was also successful in protocorms and seedlings formation and long culture maintenance.…”

Section: Discussionsupporting

confidence: 54%

Initial stages of Gymnadenia conopsea (Orchidaceae) morphogenesis in in vitro culture

Nabieva

Zhmud

2020

BIO Web Conf.

View full text Add to dashboard Cite

In the West Siberia there is very little data on the biology of cenopopulations of Gymnadenia conopsea. Habitat destruction and environmental changes, which potentially affected species long-term viability, led to G. conopsea cenopopulations fragmentation and isolation. A detailed study of the morphology variability of the Fragrant orchid representatives was carried out in the cenopopulation in Novosibirsk region. Our results indicate that asymbiotic germination of G. conopsea seeds is difficult to achieve and the species has serious conservation issues. It is the first report when the introduction of G. conopsea in tissue culture was undertaken as the initial stage of germplasm conservation of Siberian cenopopulation. Three modified nutrient media with different growth additives were tested to promote protocorm and seedling formation. The advanced G. conopsea seedlings establishment was obtained in 1/3 Murashige and Skoog (MS) medium, supplemented by 1.0 mgl−1 2-isopentenyladenine (2iP), 0.1 mgl−1 1-Naphthaleneacetic acid (NAA) and 10% coconut water. This study allowed establishing a reliable and reproducible system for the G. conopsea maintenance and conservation ex situ.

show abstract

Section: Discussionsupporting

confidence: 54%

Initial stages of Gymnadenia conopsea (Orchidaceae) morphogenesis in in vitro culture

Nabieva

Zhmud

2020

BIO Web Conf.

View full text Add to dashboard Cite

show abstract

“…Adjusting the pH of the culture medium is important to ensure the plant's physiological processes are not disturbed. Acidic medium prevents the uptake of phosphoric acid, Ca 2+ , and Mg 2+ ; alkalic medium prevents the uptake of iron, Cu 2+ , Zn 2+ , Mn 2+ , and boron (Bell et al 2020;Jakobsone and Osvalde 2019;Ichinose et al 2018). In addition, pH affects the solubility and absorption of nutrients by activating certain enzymes and solidifying gelling agents; and preventing the absorption of toxic substances (Sahu et al 2017;Lager et al 2010).…”

Section: Culture Mediamentioning

confidence: 99%

Review: Factors affecting mass propagation of Vanda orchid in vitro

Setiaji¹,

Annisa

Santoso

et al. 2021

Cell Biol Dev

View full text Add to dashboard Cite

Abstract. Setiaji A, Annisa RRR, Santoso AD, Kinasih A, Riyadi ADR. 2021. Review: Factors affecting mass propagation of Vanda orchid in vitro. Cell Biol Dev 5: 51-62. For the past decade, Vanda has been the primadonna of ornamental orchids in the south and southeast Asia, along with Phalaenopsis and Dendrobium. Along with the increase in demand for Vanda, this genus has faced several threats, from illegal collection to habitat loss. Therefore, mass propagation through in vitro culture is a promising strategy to ensure sustainable business in horticulture and conservation. This review provides an overview and synthesizes various Vanda in vitro culture literature. We showed the researchers' preferences on several aspects for growing Vanda, including species, basal medium, plant growth regulators, explant, and culture conditions. The most commonly used as explants are seeds or protocorms, growing on Murashige & Skoog or Vacin & Went medium. This medium can be added banana homogenate to increase its nutritional value. Vanda seedlings can be incubated at 25 ± 1-3oC, with a lighting intensity of 50 ?mol m-2 s-1 at 12/16 h PP. Choosing a medium that is cheaper but still rich in nutrients and its additives, especially during the subculture phase; selecting explants that are responsive and minimizing the possibility of contamination; as well as seeing the target market in particular, can make Vanda's propagation efforts more effective, efficient, and profitable.

show abstract

Peculiarities of calcium and iron effects on some wild terrestrial orchids in vitro compared to in vivo

Cited by 2 publications

References 30 publications

Initial stages of Gymnadenia conopsea (Orchidaceae) morphogenesis in in vitro culture

Initial stages of Gymnadenia conopsea (Orchidaceae) morphogenesis in in vitro culture

Review: Factors affecting mass propagation of Vanda orchid in vitro

Contact Info

Product

Resources

About