Pd34-02 Salvage Radical Prostatectomy Versus Salvage Cryotherapy for Localized Radiorecurrent Prostate Cancer: Comparative Long-Term Outcomes

Abstract

suggests that there may be a potential opportunity to bias the host response towards repair and replacement of tissue defects. This may be achieved by maneuvering host stem and progenitor cells using target specific scaffolds. In this study, we aimed to regenerate muscle functionality following volumetric muscle loss through the use of a targetspecific scaffolding system. The objectives of this study were to evaluate the functionalized decellularized tissue scaffolds on the host muscle cell migration and muscle function recovery in vivo.METHODS: Decellularized porcine scaffolds were implanted into a created defect site in the tibialis anterior muscle of rats. Three experimental groups were tested (along with two control groups); those with empty scaffolds, scaffolds seeded with muscle progenitor cells, and scaffolds injected with IGF-1 prior to implantation. To evaluate the effect of the scaffolds, a force transducer was utilized to measure isometric force produced by the tibialis anterior muscle at either 2 or 4 weeks post-implantation. To evaluate the cell infiltration of the scaffolds, the muscle and scaffold were removed and characterized by H&E and Masson 0 s Trichrome.RESULTS: Functional testing showed an increase in muscle functionality in all groups by 4 weeks relative to the defect only group. Scaffolds seeded with primary muscle cells recovered 53% of functionality by week 4, a 21% increase over the defect only group. The retrieved implants showed progressive cell infiltration of the scaffolds over time. The IGF-1 and seeded progenitor cell scaffolds yielded the greatest cell count. Fiber-like orientation of infiltrated cells within the scaffold could be seen as early as week 2.CONCLUSIONS: Our data suggest an ability of host stem cells to recruit into the scaffolds with the capability of differentiating to muscle cells, which resulted in accelerating muscle regeneration in situ. This study hopes to provide a platform for the development of tissue engineering and regenerative medicine in regards to volumetric muscle loss.

Cryosurgery Versus Primary Androgen Deprivation Therapy for Locally Recurrent Prostate Cancer After Primary Radiotherapy: A Propensity-Matched Survival Analysis

“…While there are no randomized trials to compare local salvage modalities, systematic reviews suggest similar efficacy (roughly 50% long-term biochemical control) with rates of significant toxicity (albeit with differing side-effect profiles) [3,4]. Recent propensity-matched analyses of institutional series suggest comparable efficacy between modalities such as CRYO and salvage prostatectomy [12]. However, no such comparisons exist between up-front salvage ADT and local salvage.…”