PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples

Abstract

Introduction: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. Methods: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. Results: Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. Conclusions: A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance.

Detection of vanC 1 gene transcription in vancomycin-susceptible Enterococcus faecalis

“…The cDNAs were used in the PCR amplification of the vanC 1 and vanC 2/3 genes in a final volume of 25 µL. The cDNAs were also used for PCR amplification of the 16S rRNA gene (Medeiros et al 2010). …”

Types of fraud in meat and meat products: a review.

“…Cada año se actualizan nuevas metodologías de detección e identificación de componentes en los alimentos usando por ejemplo métodos proteicos o de ADN (Ali et al, 2012;Demirhan et al, 2012) aplicado a mieles (Bruni et al, 2015); frutas y vegetales (Abdolmaleki et al, 2014;Ramos-Gomez et al, 2014;Shioda et al, 2003;Wright y Annes, 2013); en la identificación de microorganismos en alimentos (Aguado et al, 2004;Garrido-Maestu et al, 2014;Huang et al, 2015;Kadiroglu et al, 2014;Khemthongcharoen et al, 2015;Li et al, 2015;McKeague et al, 2014;Ripp et al, 2014;Rodriguez-Lazaro et al, 2014;Ruiz et al, 2014;Saiyudthong y Trevanich, 2013;Shinzato et al, 2009;Suh et al, 2014;Ubeda et al, 2009); en carnes y pescados (Demirhan et al, 2012;Keeratipibul y Techaruwichit, 2012;Maede, 2006;Osman y Nishibori, 2014;Park et al, 2007;Ripp et al, 2014). Otros métodos recientes es la técnica de Reacción en cadena de la polimerasa (PCR) la cual ha sido últimamente aplicado a la detección de microorganismos (Cremonesi et al, 2014;Delibato et al, 2014;Garrido-Maestu et al, 2014;Gattuso et al, 2014;Hong et al, 2014;Kaminska et al, 2014;Kwon et al, 2014;Mancusi y Trevisani, 2014;Medeiros et al, 2010;…”