2008
DOI: 10.1590/s0102-09352008000200003
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Abstract: RESUMOPadronizou-se uma técnica de reação em cadeia da polimerase múltipla (PCR multiplex) para detecção de Clostridium chauvoei e Clostridium septicum em culturas puras. Foram utilizados pares de iniciadores para segmentos específicos dos genes que codificam a flagelina de C. chauvoei e a toxina alfa de C. septicum. Para avaliação da PCR multiplex, foram testados 16 isolados clínicos de C. chauvoei e 15 isolados de C. septicum provenientes de ruminantes, quatro sementes vacinais de cada um desses agentes. Amo… Show more

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Cited by 5 publications
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“…Samples positive for C. chauvoei were analyzed for C. chauvoei and C. septicum using multiplex PCR techniques ( 35 ).…”
Section: Methodsmentioning
confidence: 99%
“…Samples positive for C. chauvoei were analyzed for C. chauvoei and C. septicum using multiplex PCR techniques ( 35 ).…”
Section: Methodsmentioning
confidence: 99%
“…Fragments of quadriceps femoris and masseter skeletal muscles were sampled respectively from Calves 1, and 2 from areas of characteristic blackleg lesions. These fragments were immersed in 56°C paraffin to maintain an anaerobic environment [10]; they were then refrigerated at 5°C for 20 days after which were analyzed for C. chauvoei and C. septicum using multiplex PCR techniques [12].…”
Section: Methodsmentioning
confidence: 99%
“…Samples positive for C. chauvoei were analyzed for C. chauvoei and C. septicum using multiplex PCR techniques 34 .…”
Section: The Laboratory Diagnosis Was Based Onmentioning
confidence: 99%