PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides

Jurado, Miguel; Vázquez, Covadonga; Patiño, Belén; González-Jaén, M. Teresa

doi:10.1016/j.syapm.2005.02.003

Cited by 116 publications

(65 citation statements)

References 17 publications

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“…DNA concentrations were calculated using a fluorometer (Qubit TM -Invitrogen, Buenos Aires, Argentina). Polymerase chain reaction (PCR) analyses (XP Termal Cycler, BIOR Technology CO, Hangzhou, China) using available species-specific primers for F. oxysporum (Mishra et al, 2003) and F. equiseti (Jurado et al, 2005) were made and compared with positive controls. In order to confirm the identity of F. solani isolations, the elongation factor 1-α (EF-1α) was amplified (O'Donnell et al, 1998;Geiser et al, 2004).…”

Section: Fungal Isolation and Identificationmentioning

confidence: 99%

Occurrence and distribution of soil Fusarium species under wheat crop in zero tillage

Silvestro

Stenglein

Forján³

et al. 2013

Span. j. agric. res.

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The presence of Fusarium species in cultivated soils is commonly associated with plant debris and plant roots. Fusarium species are also soil saprophytes. The aim of this study was to examine the occurrence and distribution of soil Fusarium spp. at different soil depths in a zero tillage system after the wheat was harvested. Soil samples were obtained at three depths (0-5 cm, 5-10 cm and 10-20 cm) from five crop rotations: I, conservationist agriculture (wheat-sorghum-soybean); II, mixed agriculture/livestock with pastures, without using winter or summer forages (wheat-sorghum-soybean-canola-pastures); III, winter agriculture in depth limited soils (wheat-canola-barley-late soybean); IV, mixed with annual forage (wheat-oat/Vicia-sunflower); V, intensive agriculture (wheat-barley-canola, with alternation of soybean or late soybean). One hundred twenty two isolates of Fusarium were obtained and identified as F. equiseti, F. merismoides, F. oxysporum, F. scirpi and F. solani. The most prevalent species was F. oxysporum, which was observed in all sequences and depths. The Tukey’s test showed that the relative frequency of F. oxysporum under intensive agricultural management was higher than in mixed traditional ones. The first 5 cm of soil showed statistically significant differences (p=0.05) with respect to 5-10 cm and 10-20 cm depths. The ANOVA test for the relative frequency of the other species as F. equiseti, F. merismoides, F. scirpi and F. solani, did not show statistically significant differences (p<0.05). We did not find significant differences (p<0.05) in the effect of crop rotations and depth on Shannon, Simpson indexes and species richness. Therefore we conclude that the different sequences and the sampling depth did not affect the alpha diversity of Fusarium community in this system.

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Section: Fungal Isolation and Identificationmentioning

confidence: 99%

Occurrence and distribution of soil Fusarium species under wheat crop in zero tillage

Silvestro

Stenglein

Forján³

et al. 2013

Span. j. agric. res.

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“…Identification of fungi of the genus Fusarium, based on the morphology of mycelium and macroconidia, is a reliable method but it requires time and necessary skills. The polymerase chain reaction (PCR) technique is one of the most frequently used molecular tools for rapid and sensitive identification of Fusarium species (Niessen et al 2004;Mulé et al 2005;Demeke et al 2005;Jurado et al 2005Jurado et al , 2006.…”

Section: Introductionmentioning

confidence: 99%

Early PCR-based detection of Fusarium culmorum, F. graminearum, F. sporotrichioides and F. poae on stem bases of winter wheat throughout Poland

et al. 2014

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Foot rot and crown rot are fungal diseases of wheat caused by a complex of Fusarium species. They have a huge economic impact mainly due to yield reduction. A survey was conducted to identify four Fusarium species, occurring on wheat stem bases, using speciesspecific PCR assays in samples collected during spring of 2012. The dominant species was F. graminearum, which was identified in above 64 % of samples. F. culmorum was detected in 15.71 %, F. poae in 15.71 % and F. sporotrichioides in 5.71 % wheat fields. Most of the wheat fields in the eastern Poland were infected with at least one or two of Fusarium species, while in central Poland no Fusarium species were identified in most of the fields. The presence of F. graminearum tends to favor the presence of F. culmorum and this effect was visible also for F. poae and F. sporotrichioides. The frequency of F. graminearum and F. culmorum detections were highest where wheat crops were preceded by maize and in the samples from late sown fields. The opposite observation was made for F. poae and F. sporotrichioides, where the number of detections of these species was higher in samples from early sown fields. The number of detected Fusarium species was significantly lower in samples collected from fields protected with autumn herbicide in comparison to unprotected fields. The rate of autumn N fertilization did not affect the number of Fusarium detections.

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“…In some studies, it was observed that they amplified products of different size, i.e., about 400 bp in F. graminearum , about 550 bp in F. asiaticum and about 500 bp in F. meridionale [Castañares et al, 2014;Covarelli et al, 2011]. Jurado et al [2005] developed species-specific primers for F. graminearum based on the IGS region. The Fgr-F/R primers were tested on the DNA obtained from wheat seeds with the confirmed presence of this species.…”

Section: Pcr Assaysmentioning

confidence: 99%

“…The methods of Mishra et al [2003] and Jurado et al [2005] targeted ITS and IGS of rDNA, respectively. The 175F and 430R primer set were tested in 92 isolates of 5 toxigenic Fusarium species and validated against the [Mishra et al, 2003].…”

Section: Pcr Assaysmentioning

confidence: 99%

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat

Kuzdraliński

Kot²,

Szczerba³

et al. 2017

Microb Physiol

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Infection of phyllosphere (stems, leaves, husks, and grains) by pathogenic fungi reduces the wheat yield and grain quality. Detection of the main wheat pathogenic fungi provides information about species composition and allows effective and targeted plant treatment. Since conventional procedures for the detection of these organisms are unreliable and time consuming, diagnostic DNA-based methods are required. Nucleic acid amplification technologies are independent of the morphological and biochemical characteristics of fungi. Microorganisms do not need to be cultured. Therefore, a number of PCR-based methodologies have been developed for the identification of key pathogenic fungi, such as Fusarium spp., Puccinia spp., Zymoseptoria tritici, Parastagonospora nodorum, Blumeria graminis f. sp. tritici, and Pyrenophora tritici-repentis. This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests for Fusarium graminearum, Puccinia spp., and P. tritici-repentis are insufficient.

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PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides

Cited by 116 publications

References 17 publications

Occurrence and distribution of soil Fusarium species under wheat crop in zero tillage

Occurrence and distribution of soil Fusarium species under wheat crop in zero tillage

Early PCR-based detection of Fusarium culmorum, F. graminearum, F. sporotrichioides and F. poae on stem bases of winter wheat throughout Poland

A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat

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