PCR-based methodology for the authentication of the Atlantic mackerel Scomber scombrus in commercial canned products

Infante, Carlos; Crespo, Aniela; Zuasti, Eugenia; Ponce, Marian; Pérez, Laura Crespo; Funes, Victoria; Catanese, Gaetano; Manchado, Manuel

doi:10.1016/j.foodres.2006.02.006

Cited by 26 publications

(16 citation statements)

References 18 publications

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“…Number References PCR-RLFP 71 [1, 3, 4, 6, 8, 9, 21, 23, 24, 26, 28-31, 35, 36, 38, 39, 44, 46-48, 50, 52, 54, 57-66, 70, 73, 76-78, 80, 84-88, 91, 92, 96, 100, 104-108, 112, 113, 120, 123-125, 127, 130, 133, 134, 139, 145-147, 150, 151] PCR-sequencing 43 [2, 12, 15, 17, 18, 40, 43, 45, 46, 48, 51, 53, 56, 63-67, 70, 71, 73-75, 79, 81, 82, 94, 102, 104, 107, 115, 116, 118, 124, 126, 129, 132, 136, 137, 140, 142, 148, 149] PCR-specific primers 23 [5,7,9,11,14,27,33,34,40,50,68,69,89,95,97,98,100,117,122,127,128,138,141] PCR-SSCP 9 [10,32,37,39,[109][110][111][112]144] Real-time PCR 9 [25,41,49,55,70,90,135,138,143] PCR-RAPD 5 [19,20,…”

Section: Pcr-sequencingmentioning

confidence: 99%

Molecular identification methods of fish species: reassessment and possible applications

Teletchea

2009

Rev Fish Biol Fisheries

252

192

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Fish species identification is traditionally based on external morphological features. Yet, in many cases fishes and especially their diverse developmental stages are difficult to identify by morphological characters. DNA-based identification methods offer an analytically powerful addition or even an alternative. This work intends to provide an updated and extensive overview on the PCR-methods for fish species identification. Among the ten main methods developed, three PCR-RFLP, PCR-FINS and PCR-specific primers have been the most used. Two other emerging methods, namely real-time PCR and microarray technology, offer new potential for quantification of DNA and simultaneous detection of numerous species, respectively. Almost 500 species have been targeted in the past decade, among which the most studied belong to gadoids, scombroids, and salmonids. The mitochondrial cytochrome b gene was by far the most targeted DNA markers. The most common applications belonged to the forensic, taxonomic, and ecological fields. At last, some key problems, such as the degradation of DNA, the reliability of sequences, and the use of scientific names, likely to be encountered during the development of molecular identification methods are described. In conclusion, the tremendous advances in molecular biology in the past 10 years has rendered possible the study of DNA from virtually any substrates, offering new perspectives for the development of various applications, which will likely continue to increase in the future.

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Section: Pcr-sequencingmentioning

confidence: 99%

Molecular identification methods of fish species: reassessment and possible applications

Teletchea

2009

Rev Fish Biol Fisheries

252

192

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“…The use of specific primers (or probes) has the advantage that the analysis can be performed in a short time, either by endpoint PCR (Infante et al, 2006;Hubalkova et al, 2008) or by real-time PCR (Dalmasso et al, 2007). A number of commercial kits are on the market to identify such fish species as catfish (Ictalurus punctatus), tra pangasius (Pangasius hypophthalmus), basa pangasius (Pangasius bocourti) (www.produktqualitaet.de), rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar), saithe (Pollachius virens), coal fish (Pollachius pollachius), North Atlantic hake (Merluccius merluccius), haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus), and cod (Gadus morhua) (www.coring.de).…”

Section: Raw Productsmentioning

confidence: 99%

“…Canned sardine and anchovy species, as well as herring (Clupea harengus) and sprat (Sprattus sprattus), have been differentiated recently by a protocol using sequencing of 212 to 274-bp amplicons of three mitochondrial genes (J erôme et al, 2008). A duplex-PCR assay for the authentication of Atlantic mackerel (Scomber scombrus) in commercial canned products has been developed by Infante et al (2006) using species-specific primers.…”

Section: Canned Fishmentioning

confidence: 99%

Aquatic Food

Rehbein¹

2009

Molecular Biological and Immunological Techniques and Applications for Food Chemists

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“…The use of DNA markers in the authentication process can solve some of these problems, as DNA is not susceptible to alteration due to cultivation conditions and can be obtained from a small amount of biological material, even after processed (INFANTE et al, 2006;UJIHARA et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Molecular authentication of Maytenus sp by PCR-RFLP

et al. 2013

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Maytenus aquifolia and Maytenus ilicifolia are native plants from South America and popularly known as 'Espinheira-santa'. Both are used as tea due to their efficiency in the treatment of ulcer, gastritis and indigestion. However, adulteration of processed Maytenus genus tea with Sorocea genus may happen due to their botanical similarity, compromising the quality of the products and opening a derogatory business opportunity that may lead to the discrediting of medicinal plant products. This study aimed to distinguish Maytenus sp and Sorocea bonplandii by PCR-RFLP of a chloroplast DNA (cpDNA) intergenic region. Three commercial products of processed tea leaves of Maytenus sp, and in natura leaves of Maytenus sp and S. bonplandii were analyzed. PCR detected unique fragments for all samples in natura. The trnH-psbA region amplicon of both M. ilicifolia and M. aquifolia was 660 bp, and for S. bonplandii was 565 bp. These PCR products can be used as markers to distinguish the two genera. Forty-five percent of the processed samples presented only Maytenus genus, without adulterations. However, the amplification of 38% of the samples suggests adulteration with S. bonplandii while 17% seem to be adulterated with another plant (fragment of 649 bp in brand A and 690 bp in brand B). Three out of the fifteen restriction enzymes were able to detect M. ilicifolia and M. aquifolia in natura and in processed leaf samples. It was concluded that PCR technique is efficient to distinguish Maytenus sp from S. bonplandii, and other adulterating plants in processed commercial products of 'Espinheira-santa' tea. The trnH-psbA spacer of cpDNA is easily amplified and has satisfactory discriminating capacity to help in authentication processes of samples of the genera in natura and in processed plants. Key words: Maytenus aquifolia, Maytenus ilicifolia, Sorocea bonplandii, Celastraceae, Moraceae, trnH-psbA ResumoMaytenus aquifolia e Maytenus ilicifolia são plantas nativas da América do Sul e conhecidas popularmente como "Espinheira-santa". Ambas são usadas como chá devido à sua eficiência no tratamento de gastrite, úlcera e indigestão. No entanto adulteração de chá do gênero Maytenus com o gênero Sorocea pode acontecer devido a sua semelhança botânica e isto compromete a qualidade dos produtos, abrindo uma oportunidade para comércio depreciativo que leva ao descrédito das plantas medicinais. Este estudo teve como objetivo distinguir Maytenus sp e Sorocea bonplandii por PCR-RFLP de DNA da região intergênica do cloroplasto (cpDNA). Três produtos comerciais de chá de Maytenus sp foram analisados, e a análise foi feita também em folhas in natura de Maytenus sp e de S. bonplandii. PCR detectou fragmentos únicos para todas as amostras in natura. O amplicon da região trnH-psbA de M. ilicifolia e M. aquifolia foi de 660 pb e para S. bonplandii foi de 565 pb. Os produtos de PCR podem ser usados como marcadores para distinguir os dois gêneros. Quarenta e cinco por cento das amostras processadas apresentaram apenas o gênero Maytenus, sem adu...

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PCR-based methodology for the authentication of the Atlantic mackerel Scomber scombrus in commercial canned products

Cited by 26 publications

References 18 publications

Molecular identification methods of fish species: reassessment and possible applications

Molecular identification methods of fish species: reassessment and possible applications

Aquatic Food

Molecular authentication of Maytenus sp by PCR-RFLP

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