PCR amplification of DNA sequence related to the<i>hrpD</i>gene of<i>Xanthomonas cucurbitae</i>in leaf spot disease of pumpkin and their antagonism by soil bacteria

Hasan, Sharizal; Hossain, Firose; Zaoti, Zannati Ferdous; Hasan, Faruk; Islam, Asadul; Khalekuzzaman, Md.; Sikdar, Biswanath

doi:10.1080/03235408.2018.1460923

Cited by 6 publications

(3 citation statements)

References 36 publications

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“…Also, amplification and sequencing of 16s rRNA gene resulted in 98 to 99 per cent similarity to X. cucurbitae accessions in GenBank [19]. Amplification of hrpD gene of X. cucurbitae in leaf spot disease of pumpkin was successfully carried out in isolates of X. cucurbitae [38]. PCR of X. cucurbitae with same primers resulted in 1.4 kb amplicon and gyrB rpoD fyuA dnaK resulted in isolate identity [23].…”

Section: Molecular Characterisationmentioning

confidence: 99%

“…Minimum, maximum and optimum temperatures were found to be 4-6, 34-36, and 24-30°C, respectively, and a pH of 6.5 to 7 has been reported for colony development of X. cucurbitae isolates. [38]. For growth of bacteria, an optimum temperature range of 25 to 30°C and pH range of 6-7 has been reported in case of bottle gourd and pumpkin [28].…”

Section: Cultural Studiesmentioning

confidence: 99%

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Bacterial Leaf Spot of Cucurbits: A Menace to Cultivation

Jarial¹,

Jarial²,

Bhardwaj³

et al. 2023

Biological and Abiotic Stress in Cucurbitaceae Crops

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Bacterial leaf spot is one of the important diseases of cucurbits affecting almost all the cultivated cucurbits worldwide. The disease has been reported to cause huge losses to the cucurbits as the symptoms of the disease appear on all plant parts including fruits. The disease is favoured by moderate to high temperatures and high humidity. The pathogen perpetuates in The seed and infected crop debris. The management efforts for the disease are still on and there are reports of it's management upto more than 50 per cent level under field conditions. However, the disease can be managed by the use of common antibiotics .

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Section: Molecular Characterisationmentioning

confidence: 99%

Section: Cultural Studiesmentioning

confidence: 99%

Bacterial Leaf Spot of Cucurbits: A Menace to Cultivation

Jarial¹,

Jarial²,

Bhardwaj³

et al. 2023

Biological and Abiotic Stress in Cucurbitaceae Crops

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“…The morphological and biochemical characteristics of the isolated bacteria were done. Bacteriological analysis was performed using selective media [10][11][12][13] 14 .…”

Section: Biochemical Characterizationmentioning

confidence: 99%

Molecular Detection and Biological Control of Human Hair Dandruff Causing Microorganism Staphylococcus aureus

Mas-ud¹,

Dna²,

Ali³

et al. 2020

J. Pure Appl. Microbiol.

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Human hair dandruff (HHD) is a common unwanted scalp disorder that is prevalent to most human populations all over the world. This study was designed to isolate and characterize pathogens that are responsible for HHD as well as the evaluation of their biological control technique. Isolated bacteria were characterized by different biochemical tests and molecular identification methods. Here, disc diffusion methods were used to determine antibiotic and antibacterial activity against isolated bacteria. The isolated bacterial colonies were found to be Gram-positive, small, round-shaped, and purple. PCR amplification was done using 27F and 1492R primer pairs. A BlastN search of a sequenced 1465 bp region of 16S rRNA in NCBI GenBank revealed approximately 99% genome similarity with Staphylococcus aureus. The sequence was deposited in GenBank (Accession No. MH603394). In the antibiotic sensitivity test, Kanamycin showed the highest 31.0±0.5 mm diameter zone of inhibition (DZI) against the isolated bacteria. Moreover, as a plant-derived compound, the Methanol extract of Allium sativum revealed the highest, 15.0±0.5 mm DZI. The present study would give a promising direction of identification and control of this pathogen biologically.

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