PCP-A1, a Defensin-Like Brassica Pollen Coat Protein That Binds the S Locus Glycoprotein, Is the Product of Gametophytic Gene Expression

Doughty, James; Dixon, Suzanne; Hiscock, Simon J.; Willis, Antony C.; Parkin, Isobel A. P.; Dickinson, H. G.

doi:10.2307/3870644

Cited by 37 publications

(75 citation statements)

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“…However, the position of the fourth conserved cysteine residue from the N-terminal end is different from that of class A proteins of the PCP family (8). Thus, allelic variants of SP11 form a novel class of the PCP family.…”

Section: Resultsmentioning

confidence: 99%

“…Alignment of the predicted amino acid sequences of the four allelic forms of SP11 revealed that all of their mature proteins contain eight conserved cysteine residues, one of the hallmarks of the PCP family proteins (8). However, the position of the fourth conserved cysteine residue from the N-terminal end is different from that of class A proteins of the PCP family (8).…”

Section: Resultsmentioning

confidence: 99%

“…SP11 was identified previously in the S 9 haplotype of B. campestris from sequencing analysis of the SLG 9 ͞SRK 9 region of the S locus (10): SP11 is located at the S locus, expressed exclusively in the anther, and encodes a small, cysteine-rich basic protein similar to PCP-A1, which has been shown to interact with SLG (8). Genomic DNA gel blot analyses using the SP11-9 cDNA as a probe revealed that SP11-9 hybridized either very weakly or not at all to the genomic DNA of other S haplotypes.…”

Section: Resultsmentioning

confidence: 99%

“…Digoxigenin-labeled sense and antisense RNA probes of SP11-8 (coding region) and BcPCP-A1 (coding region) were prepared by using the SP6͞T7 digoxigenin RNA labeling kit (Roche Diagnostics). In situ hybridization to 10-m-thick Paraplast (Sigma) sections of formaldehyde-fixed anthers was performed as described previously (8).…”

Section: Methodsmentioning

confidence: 99%

“…Doughty et al (7,8) analyzed the pollen coat protein (PCP) of Brassica oleracea and identified a basic 7-kDa protein, termed PCP-A1 (protein 1 of class A PCP), which specifically bound SLGs. However, because PCP-A1 binds SLGs regardless of the S haplotype and its gene is not linked to the S locus, it is unlikely to be the pollen S determinant.…”

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confidence: 99%

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The pollen determinant of self-incompatibility in Brassica campestris

Takayama

Shiba

Iwano

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

419

313

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Many flowering plants possess self-incompatibility (SI) systems that prevent inbreeding. In Brassica, SI is controlled by a single polymorphic locus, the S locus. Two highly polymorphic S locus genes, SLG (S locus glycoprotein) and SRK (S receptor kinase), have been identified, both of which are expressed predominantly in the stigmatic papillar cell. We have shown recently that SRK is the determinant of the S haplotype specificity of the stigma. SRK is thought to serve as a receptor for a pollen ligand, which presumably is encoded by another polymorphic gene at the S locus. We previously have identified an S locus gene, SP11 (S locus protein 11), of the S9 haplotype of Brassica campestris and proposed that it potentially encodes the pollen ligand. SP11 is a novel member of the PCP (pollen coat protein) family of proteins, some members of which have been shown to interact with SLG. In this work, we identified the SP11 gene from three additional S haplotypes and further characterized the gene. We found that (i) SP11 showed an S haplotype-specific sequence polymorphism; (ii) SP11 was located in the immediate flanking region of the SRK gene of the four S haplotypes examined; (iii) SP11 was expressed in the tapetum of the anther, a site consistent with sporophytic control of Brassica SI; and (iv) recombinant SP11 of the S9 haplotype applied to papillar cells of S9 stigmas, but not of S8 stigmas, elicited SI response, resulting in inhibition of hydration of cross-pollen. All these results taken together strongly suggest that SP11 is the pollen S determinant in SI.I n Brassica, SI is sporophytically controlled by a highly polymorphic locus, termed the S locus, with more than 100 haplotypes identified so far (1, 2). To date, two stigmatically expressed highly polymorphic genes have been identified at the S locus. One is the S locus glycoprotein (SLG) gene, which encodes a secreted glycoprotein abundantly present in the papillar cell of the stigma surface (3, 4), and the other is the S locus receptor kinase (SRK) gene, which encodes a putative receptor-like serine͞threonine protein kinase presumed to span the plasma membrane of the papillar cell (5). The predicted extracellular domain of SRK shares extensive sequence similarity with SLG. Results from our recent gain-of-function experiments have shown that SRK is the sole determinant of the S haplotype specificity of the stigma (6). SRK is thought to function as a receptor for the S determinant of pollen with the same S haplotype. Binding of SRK to the pollen S determinant then would elicit a signaling cascade in the papillar cell, leading to the rejection of self-pollen. Our recent study also has shown that the role of SLG is probably to enhance this recognition process, although how this is accomplished is not yet known (6).In contrast to the S determinant of the stigma, the pollen S determinant had long remained elusive. Doughty et al. (7,8) analyzed the pollen coat protein (PCP) of Brassica oleracea and identified a basic 7-kDa protein, termed PCP-A1 (protein 1 of cla...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The pollen determinant of self-incompatibility in Brassica campestris

Takayama

Shiba

Iwano

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

419

313

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Cell Adhesion, Separation and Guidance in Compatible Plant Reproduction

Mollet¹,

Faugeron²,

Morvan³

Plant Cell Separation and Adhesion

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The Developmental Biology of Pollen

Twell

2018

Annual Plant Reviews Online

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Contents X.1 Introduction Part 1: Cell biology and differentiation X.2 Microsporogenesis X.3 Microgametogenesis X.4 Sporophytic-gametophytic interactions X.4.1 The unique pollen wall X.4.2 Exine pattern mutants X.4.3 Phenylpropanoids X.4.4 The pollen coat X.4.5 Microspore nutrition and metabolism X.5 Microspore development X.5.1 Microspore isolation X.5.2 Vacuole morphogenesis, growth and polarity X.6 Asymmetric division, cell fate and polarity X.6.1 Asymmetric cytokinesis-sealing cell fate X.6.2 Microspore and pollen embryogenesis X.6.3 Models of cell-fate determination X.6.4 Determination of microspore polarity X.6.5 Mutants affecting microspore polarity, division and cell-fate X.7 Generative and sperm cell development X.7.1 Generative cell migration X.7.2 Generative cell morphogenesis X.7.3 Generative cell division X.7.4 The male germ unit X.7.4.1 Male germ unit morphogenesis Part 2: Molecular biology and genetics X.8 Male gametophytic gene expression X.8.1 Developmental phases of expression X.8.2 Transcription in developing microspores X.8.3 Transcription in developing pollen X.8.4 Pollen-specific regulatory elements X.8.5 Post-transcriptional regulation X.8.6 Gene expression in generative and sperm cells 3X.9 Male gametophytic gene functions X.9.1 Pectin degrading enzymes X.9.2 Cytoskeleton-related proteins X.9.2.1 Actin binding proteins X.9.2.2 GTPases X.9.2.3 Microtubules X.9.3 Transcriptional regulators X.9.4 Protein kinases X.9.4.1 Receptor-like kinases X.9.4.2 MAP kinases X.9.4.3 Ca 2+ -regulated protein kinases X.9.5 Pollen allergens X.9.6 Pollen wall proteins X.10 Advances in gametophytic genetics X.10.1 Pollen morphogenesis screens X.10.2 Segregation ratio distortion screens X.11 Conclusions and perspective

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PCP-A1, a Defensin-Like Brassica Pollen Coat Protein That Binds the S Locus Glycoprotein, Is the Product of Gametophytic Gene Expression

Cited by 37 publications

References 54 publications

The pollen determinant of self-incompatibility in Brassica campestris

The pollen determinant of self-incompatibility in Brassica campestris

Cell Adhesion, Separation and Guidance in Compatible Plant Reproduction

The Developmental Biology of Pollen

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