Pauferrol A, a novel chalcone trimer with a cyclobutane ring from Caesalpinia ferrea mart exhibiting DNA topoisomerase II inhibition and apoptosis-inducing activity

Nozaki, Hirōshi; Hayashi, Ken‐ichiro; Kido, Masahiro; Kakumoto, Kazuyuki; Ikeda, Shogo; Matsuura, Nobuo; Tani, Hiroyuki; Takaoka, Daisuke; Iinuma, Munekazu; Akao, Yukihiro

doi:10.1016/j.tetlet.2007.09.130

Cited by 50 publications

(34 citation statements)

References 10 publications

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“…Nakamura et al (2002) and Pereira et al (2012) reported the antitumor and anti-inflammatory properties of its fruit, which is an excellent source of energy with a large carbohydrate content that is used to feed ruminants. Nozaki et al (2007) demonstrated that C. ferrea inhibited human topoisomerase II and induced apoptosis in HL60 human leukemia cells, suggesting that this species has potential as an anti-cancer agent.…”

Section: Introductionmentioning

confidence: 97%

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

et al. 2014

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ABSTRACT. Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpinia ferrea are rare, it is important to develop and/or adapt a DNA extraction protocol that produces quality DNA samples to enable the design of strategies for the conservation of this threatened species. This study aimed to compare five methods for DNA extraction and to determine the most efficient protocol for C. ferrea. Sufficient genomic DNA was obtained from the leaves of C. ferrea using all the tested protocols to perform techniques DNA extraction from Caesalpinia ferrea involving molecular markers. Two protocols based on the detergent cetyl trimethyl ammonium bromide, as well as a commercial kit, yielded high concentrations of pure DNA. However, when polymerase chain reaction amplifications were performed, DNA was only successfully amplified from extractions performed with the commercial kit, which produced sufficient genomic DNA of good quality from the leaves of C. ferrea to perform techniques involving molecular markers.

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Section: Introductionmentioning

confidence: 97%

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

et al. 2014

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“…From stem extract of L. ferra chalcone pauferrol A, a topoisomerase II inhibitor, was isolated (Nozaki et al 2007). Different structures of metabolites of the phenolic class have already shown interaction in topoisomerase II of Leishmania species (Sen and Chattergee 2011), being this a possible mechanism of inhibitory action.…”

Section: Discussionmentioning

confidence: 99%

“…Pharmacological studies of extracts obtained from L. ferrea have demonstrated its biological anti-inflammatory (Carvalho et al 1996), bactericidal (Sampaio et al 2009), hypoglycemic and antioxidant potentials (Batubara et al 2009) and healing activity (Oliveira et al 2010). Chemical studies with this species have shown the presence of phenolic compounds with possible pharmacological applications, such as chalcones and polyphenols (Nakamura et al 2002;Nozaki et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Antileishmanial activity of extracts from Libidibia ferrea: development of in vitro and in vivo tests

et al. 2017

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Treatment of cutaneous leishmaniasis (CL) is difficult due to the scarce number of drugs able to eliminate completely the intracellular form of the parasite. In the present study, the aim was to evaluate: i) phytochemical properties of extracts from Libidibia ferrea; ii) antileishmanial activity of extracts from L. ferrea against promastigotes and amastigotes of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) guyanensis; iii) the effects of topical treatment using hydrogel containing active extract of L. ferrea on golden hamsters infected with L. (L.) amazonensis. Extracts from leaves, branches and fruits of L. ferrea were obtained with hexane and methanol and were tested by in vitro assays in promastigotes and murine macrophages J774 experimentally infected with amastigotes of Leishmania. Groups of hamsters with CL received topical treatment with a formulation of extract (10%) hydrogels, 50 mg.day ) e baixa citotoxicidade em células de macrófagos J774. Os resultados destacaram que as lesões cutâneas de animais que receberam tratamento com hidrogel + extrato (GelFrMeOH) apresentaram redução significativa (42,78%), menos úlceração e redução da carga parasitária detectada por RT-qPCR, microscopia e análise em cultura e alterações histopatológicas, como menor infiltrado inflamatório após 40 dias de tratamento. A análise química demonstrou que FrMeOH contém altos níveis de compostos fenólicos. Os resultados apontam para uma possível terapia alternativa para CL utilizando fitoterápicos. PALAVRAS-CHAVE: leishmaniose cutânea, formulações tópicas, produtos naturais, tratamento experimental

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“…Nozaki et al isolated the unique chalcone derivative 232 from the stems of C. ferrea Mart. The structure was determined on the basis of 2D-NMR spectroscopy to be a chalcone trimer, linked by a cyclobutane ring [68]. The new compound 233 was isolated from the C. decapetala for the first time [69], and compound 234 was obtained from of the stem of C. pulcherrima [2].…”

Section: Washiyama Et Al Isolated Protosappanin D (222) From Thementioning

confidence: 99%

“…Among the tested compounds, 102 was found to show a better activity profile against cervical and prostate, and breast carcinoma cell lines. Pauferrol A (232) was isolated from the stems of C. ferrea [68] and showed potent inhibitory activity against human topoisomerase II, with an IC 50 value of 2.1 mm, and cell proliferation inhibitory activity through the induction of apoptosis in human leukemia HL 60 cells, with an IC 50 value of 5.2 mm.…”

Section: Washiyama Et Al Isolated Protosappanin D (222) From Thementioning

confidence: 99%

Chemical Constituents of Plants from the Genus Caesalpinia

Wang

Zhang

et al. 2011

Chemistry & Biodiversity

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Pauferrol A, a novel chalcone trimer with a cyclobutane ring from Caesalpinia ferrea mart exhibiting DNA topoisomerase II inhibition and apoptosis-inducing activity

Cited by 50 publications

References 10 publications

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

Antileishmanial activity of extracts from Libidibia ferrea: development of in vitro and in vivo tests

Chemical Constituents of Plants from the Genus Caesalpinia

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