Patterning Cells on Complex Curved Surface by Precision Spraying of Polymers

DeSilva, Mauris N.

doi:10.1007/978-90-481-9145-1_11

Cited by 1 publication

(1 citation statement)

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“…In addition to the studies of cell differentiation and phenotype, the applications of bioprinting include: Creating specific cell-to-cell patterns that mimic in vivo patterns of cellular interaction, such as the neuronal networks [30,31] ; tool for facilitating basic biology research on specific cell-cell or cell-ECM interactions [30,32] ; cell/tissue bases sensors for chemical, drug, and toxicity testing [33] ; and tissue engineering for regenerative medicine, such as the fabrication of 2D cellular organizations that can be stacked into 3D scaffolds and, as demonstrated here, to influence stem cell differentiation on a biomaterial [7,11,14,15,28,31] .…”

Section: Discussionmentioning

confidence: 99%

A novel 3D printing method for cell alignment and differentiation

Bhuthalingam¹,

Lim²,

Irvine³

et al. 2024

IJB

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Abstract:The application of bioprinting allows precision deposition of biological materials for bioengineering applications. Here we propose a 2 stage methodology for bioprinting using a back pressure-driven, automated robotic dispensing system. This apparatus can prepare topographic guidance features for cell orientation and then bioprint cells directly onto them. Topographic guidance features generate cues that influence adhered cell morphology and phenotype. The robotic dispensing system was modified to include a sharpened stylus that etched on a polystyrene surface. The same computer-aided design (CAD) software was used for both precision control of etching and bioink deposition. Various etched groove patterns such as linear, concentric circles, and sinusoidal wave patterns were possible. Fibroblasts and mesenchymal stem cells (MSC) were able to sense the grooves, as shown by their elongation and orientation in the direction of the features. The orientated MSCs displayed indications of lineage commitment as detected by fluorescence-activated cell sorting (FACS) analysis. A 2% gelatin bioink was then used to dispense cells onto the etched features using identical, programmed co-ordinates. The bioink allows the cells to contact sense the pattern while containing their deposition within the printed pattern.

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