Patterned Thermoresponsive Microgel Coatings for Noninvasive Processing of Adherent Cells

Uhlig, Katja; Wegener, Thomas; He, Jian; Zeiser, Michael; Bookhold, Johannes; Dewald, Inna; Godino, Neus; Jaeger, Magnus S.; Hellweg, Thomas; Fery, Andreas; Duschl, Claus

doi:10.1021/acs.biomac.5b01728

Cited by 70 publications

(70 citation statements)

References 23 publications

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“…Uhlig et al designed artificial surfaces patterned with thermoresponsive microgels using nanodispensing and microcontact printing (µCP) for controlled adhesion, and cell growth of L929 mouse fibroblasts, and CHO‐K1 epithelial cells . Cell attachment, growth, and detachment from the surface were controlled by tuning the degree of swelling and elastic modulus of microgels.…”

Section: Microgels For Tissue Engineeringmentioning

confidence: 99%

“…These surfaces could be switched between a cell‐attractive and a cell‐repellent state and the grown cells could be used to perform noninvasive cell experiments. It was observed that the cells were well spread on the surface at 37 °C, while high water content, increased repulsive interactions, and unfavorable modulus at 25 °C lead to their complete detachment ( Figure ) …”

Section: Microgels For Tissue Engineeringmentioning

confidence: 99%

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Functional Microgels: Recent Advances in Their Biomedical Applications

Agrawal

2018

Small

120

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Here, a spotlight is shown on aqueous microgel particles which exhibit a great potential for various biomedical applications such as drug delivery, cell imaging, and tissue engineering. Herein, different synthetic methods to develop microgels with desirable functionality and properties along with degradable strategies to ensure their renal clearance are briefly presented. A special focus is given on the ability of microgels to respond to various stimuli such as temperature, pH, redox potential, magnetic field, light, etc., which helps not only to adjust their physical and chemical properties, and degradability on demand, but also the release of encapsulated bioactive molecules and thus making them suitable for drug delivery. Furthermore, recent developments in using the functional microgels for cell imaging and tissue regeneration are reviewed. The results reviewed here encourage the development of a new class of microgels which are able to intelligently perform in a complex biological environment. Finally, various challenges and possibilities are discussed in order to achieve their successful clinical use in future.

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Section: Microgels For Tissue Engineeringmentioning

confidence: 99%

Section: Microgels For Tissue Engineeringmentioning

confidence: 99%

Functional Microgels: Recent Advances in Their Biomedical Applications

Agrawal

2018

Small

120

View full text Add to dashboard Cite

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“…In addition, above the LCST, the microgels are hydrophobic and show a smooth surface whereas below the LCST the surface is fuzzy and the polymers are hydrated . The temperature‐control over hydrophobicity, surface structure, and stiffness was successfully used to construct microgel coatings for switchable cell culture surfaces . However, these materials do not specifically bind certain cells or bacteria but are quite promiscuous and would bind to virtually any protein, which makes it difficult to control their adhesive properties.…”

Section: Switchable Carbohydrate Presenting Scaffoldsmentioning

confidence: 99%

Interactive Polymer Gels as Biomimetic Sensors for Carbohydrate Interactions and Capture–Release Devices for Pathogens

Schmidt

Paul

Strzelczyk

2019

Macro Chemistry & Physics

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Adhesive processes mediated by carbohydrate‐decorated interfaces play a crucial role in many biological processes such as cell development or pathogen invasion. The involved carbohydrate scaffolds are soft and present multiple subsites forming complex and dynamic bonds to carbohydrate binding proteins. New tools and data are needed to understand how ligand presentation and mechanical properties drive these binding processes. This article highlights recent developments in the area of adhesion assays with a focus on soft biomimetic carbohydrate scaffolds as probes of adhesion forces. Key findings state that carbohydrate functionalized polymer networks largely show additive multivalency (statistical effects) and that the overall interaction forces are strongly affected by the stiffness of the network. These results indicate that phase transitions of carbohydrate bearing polymer gels may enable tunable affinity toward carbohydrate binding proteins. As an example, polymer networks undergoing large changes in mechanical rigidity, density, and spacing of carbohydrate ligands upon temperature stimulus are shown to bind or release carbohydrate binding bacteria such as Escherichia coli. The presented adhesion assays and the developed responsive systems can provide new insights into the mechanism through which carbohydrates mediate adhesion processes and establish new avenues toward scaffolds for the capture or release of cells or pathogens.

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“…In previous work, poly( N ‐isopropylacrylamide)‐based microgels have been used as novel thermoresponsive coatings to harvest cell/cell sheets by temperature stimulation . These work mainly focused on cell detachment behavior using the microgel monolayer, the effect of the thermoresponsive microgels distribution on cell attachment, morphology, activity, and detachment has not been well investigated.…”

Section: Introductionmentioning

confidence: 99%

The effects of thermoresponsive microgel density on cell adhesion, proliferation, and detachment

Tang

Zeng

Xia

et al. 2019

J of Applied Polymer Sci

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In recent years, poly(N‐isopropylacrylamide)‐based microgels have been emerged as a new thermoresponsive coating for cell/cell sheet harvesting, yet few work reports their effect on cell attachment, morphology, activity, and proliferation in details. In this work, poly(N‐isopropylacrylamide‐co‐styrene) (pNIPAAmSt) microgel was selected as the model to study its density on NIH3T3 cell adhesion, morphology, activity, and detachment. Results showed that 0.5 wt % pNIPAAmSt microgel density leads to more cells adhesion, higher cell activity yet lower cell proliferation. Moreover, cell adhesion location can be well controlled either by manipulating the sub‐cellular scale distances between microgels or by fabricating large scale surface patterns of the microgels on higher microgel density. By temperature stimuli, similar ratio cells detached from the microgel density surface from 0.5 to 1.5 wt %. The results in this article are worthy for the application of thermoresponsive microgels in cell regulation. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48773.

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Patterned Thermoresponsive Microgel Coatings for Noninvasive Processing of Adherent Cells

Cited by 70 publications

References 23 publications

Functional Microgels: Recent Advances in Their Biomedical Applications

Functional Microgels: Recent Advances in Their Biomedical Applications

Interactive Polymer Gels as Biomimetic Sensors for Carbohydrate Interactions and Capture–Release Devices for Pathogens

The effects of thermoresponsive microgel density on cell adhesion, proliferation, and detachment

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