Patients with nasopharyngeal carcinoma demonstrate enhanced serum and tissue ceruloplasmin expression

Abstract

The proteomics approach was adopted to study the simultaneous expression of serum proteins in patients with nasopharyngeal carcinoma (NPC). We have subjected unfractionated whole sera of ten newly diagnosed Malaysian Chinese patients with WHO type III NPC to two-dimensional gel electrophoresis (2-DE) and image analysis. The results obtained were then compared to that generated from sera of ten normal healthy controls of the same ethnic group and range of age. Our data demonstrated that the serum high abundance 2-DE protein profiles of NPC patients were generally similar to that of the controls, with exception of the ceruloplasmin (CPL) spots (identified by mass spectrometric analysis and MASCOT database search), which showed higher expression. The enhanced expression of CPL in the patients' sera was confirmed by competitive ELISA. Immunohistochemical analysis of nasopharyngeal lesions of NPC patients demonstrated moderate to strong positive CPL staining in the cytoplasm of cells at the regions of malignancy but only weak cytoplasmic staining at normal epithelial lining areas. When follow-up 2-DE and ELISA studies were performed on five of the NPC patients who responded positively to six months treatment, the difference in CPL expression was no longer significant.

Expression of high‐abundance proteins in sera of patients with endometrial and cervical cancers: Analysis using 2‐DE with silver staining and lectin detection methods

“…Taken together, the altered patterns of expression of highabundance proteins detected for EACa, SCCa and ACCa were different when compared to our previously reported protein expression profiles of patients with BrCa and NPCa [13,14], although the upregulated expression of CLU and downregulated expression of AAT have also been observed for the BrCa patients.…”

“…By using 2-DE and image analysis, we have reported the aberrant expression of high-abundance acutephase serum proteins in patients with cancers of the breast (breast carcinoma; BrCa) and nasopharynx (nasopharyngeal carcinoma; NPCa). While patients with BrCa demonstrated different altered expression of serum a 1 -antichymotrypsin (ACT), clusterin (CLU) and complement factor B [13], ceruloplasmin was the sole high-abundance protein that was upregulated in the sera of NPCa patients [14]. However, the question of whether the altered patterns of expression of the serum proteins is truly unique for the respective cancers could only be resolved if similar proteomics analyses were broadened to patients with other types of cancer.…”

Glycoproteomic analysis of WGA‐bound glycoprotein biomarkers in sera from patients with lung adenocarcinoma

“…It has many biological functions, such as roles in angiogenesis, oxidation of low-density lipoproteins, metal ion transport and homeostasis [43][44][45]. A high expression level of ceruloplasmin has been reported in various cancers, such as ovarian cancer, hepatocarcinoma and nasopharyngeal carcinoma [46][47][48]. In lung cancer, the ceruloplasmin gene was found to be overexpressed in adenocarcinoma and squamous cell carcinoma [49], which was in agreement with our results of up-regulated ceruploplasmin in lung adenocarcinoma serum samples.…”

Plasma proteomic analysis of patients with squamous cell carcinoma of the uterine cervix

“…17 Increased expression of ceruloplasmin has been reported in the serum of several cancer patients. 12,[20][21][22] Complement components play a central role as mediators of inflammation and regulate of the immune response. 23 Complement C3, the third component of complement, is not only the most abundant complement protein in human serum, but also plays a central role in the complement system.…”

“…Between plasma and serum, plasma tends to be preferable for many laboratory investigators, as the components of plasma better reflect the pathological status of a patient than serum 10,11 To identify new cancer biomarkers, proteomic analysis with two dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) have been used to examine the differential expression of protein in serum or plasma specimens from different tumors, such as nasopharyngeal carcinoma, gastric cancer, and hepatocellular carcinoma. [12][13][14] The advantage of 2-DE combined with MALDI-MS is that it can detect differences in the expression levels of various proteins between healthy normal controls and cancer patients both qualitatively and quantitatively. 15 This study was performed to evaluate the expression of plasma proteins using 2-DE combined with MALDI-MS in patients with squamous cell carcinoma (SCC) of the uterine cervix and patients with benign gynecologic disease as normal controls.…”