Pathotypes of Bacterial Spot Pathogen Infecting Capsicum Peppers in Korea

Abstract

Sixty-seven isolates of bacterial spot pathogen (Xanthomonas spp.) collected from six provinces of Korea were tested for the identification of their pathotypes and determination of their distribution throughout Korea in an effort to genetically manage the disease. Near isogenic lines of Early Calwonder (Capsicum annuum) pepper plants carrying Bs1, Bs2 and Bs3, and PI235047 (C. pubescens) were used as differential hosts. Race P1 was found to be predominant, followed by race P7, and races P3 and P8 were also observed. This is the first report of races P7 and P8 in Korea. The races P7 and P8 were differentiated from the former races P1 and P3, respectively, on the basis of their ability to elicit hypersensitive reactions to PI235047.

Transcriptome analysis reveals defense-related genes and pathways against Xanthomonas campestris pv. vesicatoria in pepper (Capsicum annuum L.)

“…due to hypersensitive response reaction to Xcv containing avrBs1, while non-hypersensitive reaction in response to infiltration of the bacterial suspension into leaf tissues appeared in ECW [43], which was consistent with the results in this study (Fig 1). However, the molecular mechanism of VI037601 and ECW in response to Xcv infection was unclear.…”

Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics

“…These genes were found in PI163192 (Bs1, C. annuum), PI260535 (Bs2, C. chacoense), PI271322 (Bs3, C. annuum), PI235047 (Bs4, C. pubescens) and UNEF1556 (Bs7, C. baccatum var. pendulum [39]. Moreover, two recessive genes (bs5 and bs6), that govern a non-hypersensitive resistance and act additively with each other, were discovered in PI271322, Pep13 and PI163192 (C. annuum) [38,40].…”

Characterization of Sources of Resistance to Bacterial Spot in Capsicum Peppers

“…고추의 세균성점무늬병에는 X. euvesicatoria가 주요 원인 균종인 것으로 보인다 (Jones et al, 1998(Jones et al, , 2004EPPO, 2013). X. euvesicatoria의 병원성 변이형(race)은 기주 식물의 과민반응형 저항성(hypersensitive resistance) 유전자와의 특이적 반응에 따라 현재까지 race 0부터 race 10까지 분류하고 있으나 (Sahin and Miller, 1998;Kousik and Ritchie, 1999;Stall et al, 2009) 국내에서는 오랜 기간 race 1과 race 3만 알려졌으며 (Kim et al, 1990;Pae et al, 1994;Lee and Cho, 1996), 최근 Bs4 유전자를 보유한 PI235047을 이용하여 race 7과 race 8까지 분류되었다 (Wai et al, 2015).…”

“…세균성점무늬병에 대한 저항성은 미국식물도입국(PI) 계통들에서 고도의 저항성이 보고되었는데 (Sowell, 1960), 이들 계통 중 PI163192, PI260435, PI271322, PI235047에서 발견된 과민반응형 저항성 유전자는 각각 Bs1, Bs2, Bs3, Bs4로 명명되었 다. 이들의 과민반응형(hypersensitive reaction, HR) 저항성은 병원균이 침입하면 즉각적으로 침입부위를 중심으로 조직이 괴 사하면서 방어조직을 형성하여 병의 진행을 막는 현상으로서 각 계통의 유전양식은 서로 독립적으로 단일 우성유전자에 지배 되며 Stall, 1963, 1969;Cook and Guevara, 1984;Kim and Hartmanm, 1985;Hibberd et al, 1987;Sahin and Miller, 1998), 유전자-유전자 양식(gene-for-gene model)에 따라 병원세균에 들어있는 비병원성 유전자(avirulence gene) 와의 상호작용에 따라 과민형 반응을 나타낸다 (Minsavage et al, 1990;Stall et al, 2009;Wai et al, 2015). 이러한 주동 유전자 는 여교잡법으로 실용품종에 도입하기 쉬우나 새로운 race에 쉽게 무력화되는 문제가 있다.…”