Pathophysiological effects of Klebsiella pneumoniae infection on Galleria mellonella as an invertebrate model organism

Sugeçti, Serkan

doi:10.1007/s00203-021-02346-y

Cited by 18 publications

(7 citation statements)

References 44 publications

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“…The genus Tetrasphaera is involved in enhanced biological phosphorus removal (Nielsen et al, 2019). Tetrasphaera may be able to influence the biological behavior of K. pneumoniae by regulating the total amount of phosphorus (Sugecţi, 2021). One study reported that Leuconostoc lactis can cause liver abscess formation (Vagiakou-Voudris et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Metagenome Analysis of the Bacterial Characteristics in Invasive Klebsiella Pneumoniae Liver Abscesses

Zhang

Wang

Guo

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundKlebsiella pneumoniae liver abscess (KPLA) combined with extrahepatic migratory infection (EMI) is defined as invasive KPLA (IKPLA) and is associated with a poor prognosis. The mechanism of IKPLA formation is yet to be elucidated. In this study, metagenomic sequencing was used to compare the bacterial characteristics between IKPLA and KPLA to explore the underlying mechanism of invasiveness.MethodsClinical details, imaging, and microbial features were retrospectively evaluated by medical record review. Metagenomic sequencing was performed on the pus samples of liver abscesses whose culture results were indicative of monomicrobial Klebsiella pneumoniae (K. pneumoniae). Bacterial diversity and composition in IKPLA and KPLA were comparatively analyzed, and the key pathways and genes that may affect invasiveness were further explored.ResultsSixteen patients were included in this study. Five patients with EMI were included in the IKPLA group, and the other eleven patients without EMI were assigned to the KPLA group. There was no statistical difference in the hypermucoviscous phenotype and serotype of K. pneumoniae between the two groups. The bacterial diversity of IKPLA was lower than that of KPLA. The abundant taxa in the IKPLA group were primarily species of unclassified Enterobacteriaceae and K. pneumoniae. The KPLA group had a high abundance of the genera Tetrasphaera and Leuconostoc. Metabolic pathway genes represented most of the enriched genes in IKPLA. Fourteen pathogenic genes with significant differences in abundance were identified between the two groups, including ybtS, fepC, phoQ, acrB, fimK, magA, entC, arnT, iucA, fepG, oqxB, entA, tonB, and entF (p < 0.001).ConclusionThe diversity and bacterial composition of IKPLA were significantly different from those of KPLA. Microbiological changes in the abscess, activation of the related metabolic pathways, and the pathogenic gene expression may constitute a novel mechanism that regulates the invasiveness of KPLA.

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Section: Discussionmentioning

confidence: 99%

Metagenome Analysis of the Bacterial Characteristics in Invasive Klebsiella Pneumoniae Liver Abscesses

Zhang

Wang

Guo

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Stress can cause changes on erythrocytes and biochemical parameters (eg glucose, catecholamine, enzyme activity, and cortisol etc.) (Sugeçti, 2021a;Sugeçti, 2021b;Witeska et al, 2022). There is an increase in MCV and Ht values due to stress.…”

Section: Various Factors That Can Affect Blood Biochemistrymentioning

confidence: 99%

Blood Sampling Techniques and Preparing for Analysis in Rainbow Trout (Oncorhynchus mykiss)

Duran

Çenesiz

ŞAHİN

2023

Black Sea Journal of Agriculture

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In aquaculture, biochemical and hematological analyzes are frequently performed for scientific research, health screening and diagnosis of diseases. Biochemical and hematological parameters in fish as in other vertebrates varies nutrition, water quality, pathogens and various environmental factors that can create stress. Caudal vascular blood collection is a non-invasive method that is widely used to investigate fish health, biochemistry, and physiology. As a result of this method being performed under the influence of a properly selected anesthetic agent, animal welfare is affected the least, thus preventing a serious change in biochemical parameters with minimum stress. In this review, sampling collection suitable for the analysis to be performed and choosing the right anesthetic agent are presented.

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“…author reported that ions such as Mg, Ca, and K due to this damage were suppressed (Sugeçti 2021a). In another study, the effects of organophosphate insecticide dichlorvos (at concentrations of 2, 4, 6, and 8 µg per 100 g diet) on G. mellonella investigated.…”

Section: Acknowledgmentmentioning

confidence: 99%

“…Metabolic enzymes such as alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), creatine kinase (CK) and antioxidants such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) eliminate oxidative damage (Sugeçti and Büyükgüzel, 2018;Pisoschi and Pop, 2015;Coskun et al, 2020). In addition, metabolic enzymes and amount of ions have important roles in the regulation of energy metabolism and homeostasis (Kayis et al, 2015;Sugeçti, 2021a) Galleria mellonella is an important pest belonging to the order Lepidoptera. G. mellonella is a pest that causes significant damage to beehives (Kwadha et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of Ni (II) p-hydroxybenzoate with caffeine on metabolic, antioxidant and biochemical parameters of model insect Galleria mellonella L. (Lepidoptera: Pyralidae)

2021

Turk J Zool

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Many insecticides used against pests cause environmental pollution. Therefore, it is very important to develop new alternative strategies to toxic insecticides. In this study, the physiological and biochemical effects of Ni (II) p-hydroxybenzoate with caffeine on the model insect Galleria mellonella were investigated. In the present study, transferase enzyme activities such as alanine transferase (ALT), aspartate transferase (AST) and gamma-glutamyl transferase (GGT), which regulate amino acid metabolism, were altered in hemolymph of G. mellonella larvae exposed to Ni (II) p-hydroxybenzoate with caffeine. In addition, the activities of metabolic enzymes such as creatine kinase (CK), lactate dehydrogenase (LDH) and amylase (AMYL) increased in hemolymph of G. mellonella larvae. Amounts of the biochemical parameters such as uric acid (UA), bilirubin (BIL), albumin (ALB), cholesterol (CHOL) and total protein (TP) were increased exposed to Ni (II) p-hydroxybenzoate with caffeine. Based on these results, we can suggest a well-adjusted of Ni (II) p-hydroxybenzoate with caffeine concentrations as a potential alternative chemical for pest control.

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Pathophysiological effects of Klebsiella pneumoniae infection on Galleria mellonella as an invertebrate model organism

Cited by 18 publications

References 44 publications

Metagenome Analysis of the Bacterial Characteristics in Invasive Klebsiella Pneumoniae Liver Abscesses

Metagenome Analysis of the Bacterial Characteristics in Invasive Klebsiella Pneumoniae Liver Abscesses

Blood Sampling Techniques and Preparing for Analysis in Rainbow Trout (Oncorhynchus mykiss)

Effects of Ni (II) p-hydroxybenzoate with caffeine on metabolic, antioxidant and biochemical parameters of model insect Galleria mellonella L. (Lepidoptera: Pyralidae)

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