Pathological and immunohistochemical evaluation of skin and teat papillomas in cattle

Beytut, Enver

doi:10.3906/vet-1609-65

Cited by 11 publications

(7 citation statements)

References 25 publications

(81 reference statements)

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“…There was also epidermal maculopapular evidence of an erosive epidermal lesion. These re-sults concurred with those reported elsewhere [17]. The affected keratinocytes had larger nuclei, eosinophilic intracytoplasmic inclusion bodies, and vacuolation.…”

Section: Discussionsupporting

confidence: 92%

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Khaleefah,

Al-Tameemi,

Kraidi

et al. 2024

Korean J Vet Res

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Bird species, particularly poultry and other bird types, including domestic pigeons, are susceptible to fowl pox, a contagious viral disease. The main goal of this study was to validate clinical avipoxvirus diagnoses using molecular analytical methods. The essential components of the investigation were the clinical signs, visible abnormalities, histological changes, and polymerase chain reaction analysis. Twenty out of 120 pigeons had clinical symptoms, which included yellowish crust or nodules near the feet, eyes, and beak. An erosive epidermal lesion and an epidermal acanthotic papular lesion with basal vacuolation were maculopapular evidence associated with significant epidermal hyperkeratosis, as confirmed by histological analysis. In addition, the results showed keratinocyte necrosis beneath the hyperkeratotic epidermal layer, together with superficial and deep dermal perivascular lymphocytic infiltration. In addition, the P4b core protein gene underwent phylogenetic analysis. The sequence analysis results indicated a high degree of similarity across the local strains, with just minor variations observed. Five sample sequences were selected and submitted to the NCBI database. These sequences were identified as OR187728, OR187729, OR187730, OR187731, and OR187732. All the various strains in this research may be classified under clade A of the chicken pox virus phylogenetic classification. This study presents the first description and characterization of pox virus infections in domestic pigeons inside the Basrah governorate.

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Section: Discussionsupporting

confidence: 92%

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Khaleefah,

Al-Tameemi,

Kraidi

et al. 2024

Korean J Vet Res

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“…However, despite the wide variety of BPVs identified in this study, the same papilloma-associated histological changes were observed in all the samples, and there was no correlation between the gross pattern and the BPV type identified. Although not considered pathognomonic of BPV infection, the presence of koilocytes observed in the histological analysis likely indicates the cytopathic effect of the papillomavirus in the tissues [2,5]. PCR assays using the FAP59/FAP64 degenerate primers to amplify partial fragments of the BPV L1 gene, followed by sequencing of the amplified product, is an important tool that is widely used to determine the presence of different BPV types in cattle herds from different geographical regions [1,10,18,21,23,25].…”

Section: Discussionmentioning

confidence: 99%

“…Papillomas may be large enough to interfere with the milking process and milk flow, especially when they are located near the sphincter of the teat, predisposing the cattle to mastitis. In addition, ulceration and rupture of the lesions may result in bleeding and distortion of the lactiferous ducts [5,6,8,15,19,25].…”

Section: Introductionmentioning

confidence: 99%

Molecular and pathological characterization of teat papillomatosis in dairy cows in southern Brazil

et al. 2019

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Teat papillomatosis is caused by different bovine papillomavirus (BPV) types and is especially important for dairy cows, because it results in severe damage to the health and structure of the mammary gland. This work describes the molecular and pathological aspects of teat papillomatosis in dairy cows in southern Brazil. Samples of teat papillomas were collect from 73 slaughtered dairy cows. Fragments of the lesions were collected in individual pools per animal and subjected to PCR using the FAP primer pair and sequencing of the amplification products. Teats with the remaining lesions were fixed in 10% neutral buffered formalin, routinely processed for histopathology, and stained with hematoxylin and eosin (H&E). Papillomatous lesions were characterized by three macroscopic patterns, namely exophytic (5 [6.9%]), flat (29 [39.7%]), and mixed (39 [53.4%]). Histologically, all samples were identified as squamous papillomas. Partial sequencing of the L1 gene resulted in the detection of 8 classical BPV types (BPVs 4,

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“…In Beytut (2017), on the other hand, observed widespread epidermal hyperplasia, acanthosis and hyperkeratosis, as well as an increase in connective tissue in the dermis. However, koilocytes have seen balloon-like degeneration, pycnotic and nuclear fragmentation containing dense chromatin.…”

Section: Discussionmentioning

confidence: 97%

Phylogenetic Analysis And Searching Bovine Papillomaviruses In Teat Papillomatosis Cases In Cattle By Histopathological, Immunohistochemical And Transmission Electron Microscopy Methods

Yıldırım

Kale

Özmen

et al. 2021

Preprint

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Papillomaviruses are epitheliotropic viruses causing proliferations in skin, mucosa and various internal organs in different animal species. Especially due to lesions it causes in teats of cattle, it leads to important economical losses in milk sector. In this study, the aim was to diagnose bovine papillomaviruses (BPVs) causing teat papillomas in cattle by immunohistochemical, transmission electron microscopy (TEM) and molecular methods and to detect the defect on tissues by the virus using histopathological method. In addition to this, sequence analysis of the isolated field strains were to be carried out and their genetic and phylogenetic closeness with the strains within the literature were to be detected. After confirming teat papillomatosis in the collected samples using histopathological and immunohistochemical methods, other diagnosis methods were then used. During the TEM examination of teat lesions, intranuclear virus particles were seen in epithelium cells. After carrying out PCR using degenerate primers and type specific primers, 7 samples were detected as positive for BPV and these samples were used for typing using sequence analysis/PCR with type-specific primers. Within these analysis, three out of seven BPV isolates we collected from infected teat tissues of different cattle were detected as BPV-6, two as BPV-10, one as BPV-2 and one as BPV-8. Five isolates we isolated during sequence analysis of positive samples were found in Xipapillomavirus 1 genus, one in Epsilonpapillomavirus 1 genus and another in Deltapapillomavirus genus. As a result, in molecular diagnosis of BPV that takes place in etiology of teat papillomas, using type specific primers proved to be useful following the usage of genotyping in molecular diagnosis of BPV and generate primers in characterization. Detecting BPV types and their prevalence, taking biosafety measures in animal breeding and giving importance to vaccine studies was considered essential.

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Pathological and immunohistochemical evaluation of skin and teat papillomas in cattle

Cited by 11 publications

References 25 publications

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Clinical and molecular detection of fowl pox in domestic pigeons in Basrah Southern of Iraq

Molecular and pathological characterization of teat papillomatosis in dairy cows in southern Brazil

Phylogenetic Analysis And Searching Bovine Papillomaviruses In Teat Papillomatosis Cases In Cattle By Histopathological, Immunohistochemical And Transmission Electron Microscopy Methods

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