Pathogens in Pacific white shrimp (Litopenaeus vannamei Boone, 1931) and their relationship with physicochemical parameters in three different culture systems in Tamaulipas, Mexico

Gutiérrez-Salazar, Gilberto; Molina-Garza, Zinnia Judith; Hernández-Acosta, Mario; García-Salas, Juan A.; Mercado-Hernández, Roberto; Galaviz‐Silva, Lucio

doi:10.1016/j.aquaculture.2011.08.032

Cited by 13 publications

(6 citation statements)

References 28 publications

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“…It is therefore logical that its evolution is significantly correlated with Chl a concentration ( p = 0.004). However, Leucotrix mucor is known to cause fouling disease in shrimps, although it is not considered to be a true pathogen [ 12 ]. The family Oceanospirillaceae representing 43.1 % before the beginning of the experiment, decreased significantly after 2 days of rearing (1.2 %).…”

Section: Resultsmentioning

confidence: 99%

Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

Cardona¹,

Gueguen²,

Magré³

et al. 2016

BMC Microbiol

213

151

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BackgroundBiofloc technology (BFT), a rearing method with little or no water exchange, is gaining popularity in aquaculture. In the water column, such systems develop conglomerates of microbes, algae and protozoa, together with detritus and dead organic particles. The intensive microbial community presents in these systems can be used as a pond water quality treatment system, and the microbial protein can serve as a feed additive. The current problem with BFT is the difficulty of controlling its bacterial community composition for both optimal water quality and optimal shrimp health. The main objective of the present study was to investigate microbial diversity of samples obtained from different culture environments (Biofloc technology and clear seawater) as well as from the intestines of shrimp reared in both environments through high-throughput sequencing technology.ResultsAnalyses of the bacterial community identified in water from BFT and “clear seawater” (CW) systems (control) containing the shrimp Litopenaeus stylirostris revealed large differences in the frequency distribution of operational taxonomic units (OTUs). Four out of the five most dominant bacterial communities were different in both culture methods. Bacteria found in great abundance in BFT have two principal characteristics: the need for an organic substrate or nitrogen sources to grow and the capacity to attach to surfaces and co-aggregate. A correlation was found between bacteria groups and physicochemical and biological parameters measured in rearing tanks. Moreover, rearing-water bacterial communities influenced the microbiota of shrimp. Indeed, the biofloc environment modified the shrimp intestine microbiota, as the low level (27 %) of similarity between intestinal bacterial communities from the two treatments.ConclusionThis study provides the first information describing the complex biofloc microbial community, which can help to understand the environment-microbiota-host relationship in this rearing system.

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Section: Resultsmentioning

confidence: 99%

Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

Cardona¹,

Gueguen²,

Magré³

et al. 2016

BMC Microbiol

213

151

View full text Add to dashboard Cite

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“…GU451651). The epibiont Leucothrix mucor is the most studied member of this genus and is known to cause fouling diseases in prawns, although it is not considered to be a true pathogen [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular Analysis of Bacterial Communities and Detection of Potential Pathogens in a Recirculating Aquaculture System for Scophthalmus maximus and Solea senegalensis

et al. 2013

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The present study combined a DGGE and barcoded 16S rRNA pyrosequencing approach to assess bacterial composition in the water of a recirculating aquaculture system (RAS) with a shallow raceway system (SRS) for turbot (Scophthalmus maximus) and sole (Solea senegalensis). Barcoded pyrosequencing results were also used to determine the potential pathogen load in the RAS studied. Samples were collected from the water supply pipeline (Sup), fish production tanks (Pro), sedimentation filter (Sed), biofilter tank (Bio), and protein skimmer (Ozo; also used as an ozone reaction chamber) of twin RAS operating in parallel (one for each fish species). Our results revealed pronounced differences in bacterial community composition between turbot and sole RAS, suggesting that in the systems studied there is a strong species-specific effect on water bacterial communities. Proteobacteria was the most abundant phylum in the water supply and all RAS compartments. Other important taxonomic groups included the phylum Bacteriodetes. The saltwater supplied displayed a markedly lower richness and appeared to have very little influence on bacterial composition. The following potentially pathogenic species were detected: Photobacterium damselae in turbot (all compartments), Tenacibaculum discolor in turbot and sole (all compartments), Tenacibaculum soleae in turbot (all compartments) and sole (Pro, Sed and Bio), and Serratia marcescens in turbot (Sup, Sed, Bio and Ozo) and sole (only Sed) RAS. Despite the presence of these pathogens, no symptomatic fish were observed. Although we were able to identify potential pathogens, this approach should be employed with caution when monitoring aquaculture systems, as the required phylogenetic resolution for reliable identification of pathogens may not always be possible to achieve when employing 16S rRNA gene fragments.

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“…These environmental parameters are important factors in ascertaining the transmission of pathogens and environmental persistence (disease ecology). They are usually employed in projecting and preventing cases of infectious disease outbreaks arising from waterborne pathogens [39,40].…”

Section: Discussionmentioning

confidence: 99%

Distribution and Public Health Significance of Vibrio Pathogens Recovered from Selected Treated Effluents in the Eastern Cape Province, South Africa

Osunla

Abioye

Okoh

2021

Water

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Treated sewage harbours pathogenic microbes, such as enteric bacteria and protozoa, are capable of causing several diseases. Some of these are emerging pathogens sometimes recovered in the absence of common water quality indicator organisms. The possibility of selected treatments plants serving as momentary reservoirs of Vibrio pathogens during a non-outbreak period was assessed. The occurrence and diversity of Vibrio pathogens were monitored for one year (December 2016 to November 2017) in the treated effluents and upstream and downstream areas of the receiving water bodies of two wastewater treatment plants (WWTPs), designated AL and TS. Physicochemical parameters of TS and AL WWTPs’ water samples were analysed using a multi-parameter meter (Hanna, model HI 9828, Padova, Italy) and a turbidimeter (HACH, model 2100P, Johannesburg, South Africa). Water samples were augmented with alkaline peptone water and cultured on thiosulfate citrate bile salts sucrose agar at 37 °C for 24 h. The recovered probable pathogens were confirmed via PCR amplification, using primers specific for Vibrio species of public health significance. The distribution of Vibrio species positively and significantly (p < 0.01) correlated with turbidity (r = 0.630), temperature (r = 0.615), dissolved oxygen (r = 0.615), pH (r = 0.607), biological oxygen demand (r = 0.573), total dissolved solid (r = 0.543), total suspended solid (r = 0.511), electrical conductivity (r = 0.499), residual chlorine (r = 0.463) and salinity (r = 0.459). The densities of Vibrio species were found to be significantly higher (p < 0.05) in effluents from both AL and TS WWTPs than upstream and downstream of the receiving rivers across the sampling regime. Furthermore, the maximum Vibrio species density across the sampling regime were observed during the warmer Summer and Spring season. Moreover, six medically important Vibrio species were detected in the water samples, indicating that the methods employed were efficient in revealing that WWTPs are potential reservoirs of Vibrio pathogens, which could pose a substantial public health risk if the receiving water is used for domestic purposes. Our findings further strengthen existing calls for the inclusion of emerging bacterial pathogens, including Vibrio species, as water quality indicators by the South African Department of Water Affairs. Hence, we recommend regular monitoring of treated effluents and receiving water bodies to ensure early control of potential outbreaks of vibriosis and cholera.

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Pathogens in Pacific white shrimp (Litopenaeus vannamei Boone, 1931) and their relationship with physicochemical parameters in three different culture systems in Tamaulipas, Mexico

Cited by 13 publications

References 28 publications

Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

Molecular Analysis of Bacterial Communities and Detection of Potential Pathogens in a Recirculating Aquaculture System for Scophthalmus maximus and Solea senegalensis

Distribution and Public Health Significance of Vibrio Pathogens Recovered from Selected Treated Effluents in the Eastern Cape Province, South Africa

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