Pathogenicity of Mycoplasma synoviae in Chicken Tracheal Organ Cultures

Hirano, Haruko; Hayatsu, Eizo; Kume, Katsumi; Kawakubo, Yasuaki; Nakagawa, Susumu; Nishiyama, Yasukazu; Yoshioka, Morimasa

doi:10.1292/jvms1939.40.147

Cited by 7 publications

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“…The inoculated plates were incubated at 37 C for 3 to 7 days in humid air and the colonies were counted. Determination of ciliary actiuity, Ciliary activity of the tracheal rings was determined by the method described by Hirano et al (14). The tubes were observed daily to determine the presence or absence of ciliary motion (10).…”

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Comparison of Ciliostasis by Mycoplasmas in Mouse and Chicken Tracheal Organ Cultures

Araake

1982

Microbiology and Immunology

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The cilia-stopping effect of mycoplasmas of human and various animal origin in mouse and chicken tracheal organ cultures was studied. From the results in mouse tracheal organ cultures, the mycoplasma strains tested were divided into three groups: Mycoplasma pulmonis m53, M pulmonis JB, M. pulmonis OK, M. mycoides subsp. Mycoides PGl and M. Gallisepticum S6 showed a strong cilia-stopping effect; M. pulmonis PG22, M. mycoides subsp. capri PG3, M. meleagridis 19729, M. neurolyticum Type A and M. arthritidis PG6 showed a mild effect; and M. pneumoniae FH, M. salivarium Hup, M. hominis type 1-C and M. orale N-C human origin and Acholeplasma laidlawii PG8 showed a weak effect. On the other hand, in chicken tracheal organ cultures, only M. gallisepticum S6 showed a strong effect, M. meleagridis 19729 was affected to a lesser degree, and the other mycoplasma strains showed a weak or no effect. The results indicate that some murine and poultry mycoplasmas showed a cilia-stopping tendency in mouse and chicken tracheal organ cultures, respectively, while human mycoplasmas showed weak or little effect in both organ cultures. In mouse tracheal organ cultures, M. pulmonis m53 treated with heat, trypsin or formaldehyde, and the sterile filtrate of an m53 broth culture showed no cilia-stopping effect. The relationship of the pathogenicity of mycoplasmas for their natural hosts to that for cultured respiratory cells is discussed.

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Comparison of Ciliostasis by Mycoplasmas in Mouse and Chicken Tracheal Organ Cultures

Araake

1982

Microbiology and Immunology

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Mycoplasmal Respiratory Infections

Cassell¹,

Clyde²,

Davis³

1985

Mycoplasma Pathogenicity

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Characterization and Pathogenicity of Hemolysis Mutants of Mycoplasma pneumoniae

Yayoshi¹,

Araake²,

Hayatsu

et al. 1984

Microbiology and Immunology

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Hemolysis mutants were produced by treating Mycoplasma pneumoniae FH‐P24 strain with N‐methyl‐N‐nitro‐nitrosoguanidine and were classified into three different groups. The first group of mutants, strains P24‐L1, L2, and L11, showed wide and clear hemolytic zones. Their attachment ability to erythrocytes of various animals and to hamster lung cells were the same as those of the parent strain. The second group, strain P24‐S1, showed non‐hemolysis and non‐hemadsorption, but retained the attachment ability to lung cells, although not to erythrocytes. The third group, strain P24‐S11, was non‐hemolytic, had completely lost the attaching ability, and did not proliferate in vivo. Strains in the first group produced significant microscopic pneumonic lesions in hamsters while strain P24‐S1 produced milder lung lesions. Strain P24‐S11 did not cause any lung lesions, and organisms were not recovered from the lungs of hamsters. The attachment of M. pneumoniae to respiratory epithelium as a cause of infection and the existence of a relationship between the hemolytic abilities of the organisms and histopathogenicity in the hamster lung tissue were further supported by the present data. It was also shown that the use of hemolysis mutants is useful for the elucidation of pathogenesis in mycoplasmal infections.

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Pathogenicity of Mycoplasma synoviae in Chicken Tracheal Organ Cultures

Cited by 7 publications

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Comparison of Ciliostasis by Mycoplasmas in Mouse and Chicken Tracheal Organ Cultures

Comparison of Ciliostasis by Mycoplasmas in Mouse and Chicken Tracheal Organ Cultures

Mycoplasmal Respiratory Infections

Characterization and Pathogenicity of Hemolysis Mutants of Mycoplasma pneumoniae

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