Pathogenesis and Pathological Changes of Avian Influenza in Human

Feng, Yinchang; Le, Xiaohua; Wu, Sihan

doi:10.1007/978-981-16-1429-3_4

Cited by 3 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In most cases, influenza A (IAV) infects tracheal cells and as well the bronchial epithelial cells, causing localised cellular damage and inducing an acute inflammatory response within the host [17]. Berberine alkaloid was studied in vitro for their effect on IAV infections.…”

Section: Antiviral Activities Of Alkaloids Against Multiple Virusesmentioning

confidence: 99%

Alkaloids As Potential Antiviral : A Comprehensive Review

2024

IJPREMS

View full text Add to dashboard Cite

show abstract

Section: Antiviral Activities Of Alkaloids Against Multiple Virusesmentioning

confidence: 99%

Alkaloids As Potential Antiviral : A Comprehensive Review

2024

IJPREMS

View full text Add to dashboard Cite

show abstract

“…Seasonal influenza A strains generate major morbidity and economic losses around the world each year. In most cases, influenza A (IAV) infects tracheal cells and as well the bronchial epithelial cells, causing localised cellular damage and inducing an acute inflammatory response within the host [ 17 ].…”

Section: Antiviral Activities Of Alkaloids Against Multiple Virusesmentioning

confidence: 99%

Alkaloids as potential antivirals. A comprehensive review

Faisal

Badshah

Kubra

et al. 2023

Nat. Prod. Bioprospect.

View full text Add to dashboard Cite

Alkaloids are a diverse group of natural phytochemicals. These phytochemicals in plants provide them protection against pests, and herbivorous organisms and also control their development. Numerous of these alkaloids have a variety of biological effects, and some have even been developed into medications with different medicinal properties. This review aims to provide a broad overview of the numerous naturally occurring alkaloids (isolated from both terrestrial and aquatic species) along with synthetically produced alkaloid compounds having prominent antiviral properties. Previous reviews on this subject have focused on the biological actions of both natural and synthetic alkaloids, but they have not gone into comprehensive detail about their antiviral properties. We reviewed here several antiviral alkaloids that have been described in the literature in different investigational environments i.e. (in-vivo, in-ovo, in-vitro, and in-silico), and found that these alkaloid compounds have significant antiviral properties against several infectious viruses. These alkaloids repressed and targeted various important stages of viral infection at non-toxic doses while some of the alkaloids reported here also exhibited comparable inhibitory activities to commercially used drugs. Overall, these anti-viral effects of alkaloids point to a high degree of specificity, implying that they could serve as effective and safe antiviral medicines if further pursued in medicinal and pharmacological investigations.

show abstract

Expression of Influenza M2e-NP Recombinant Fusion Protein in Escherichia coli BL21 (DE3) and Its Binding to Antibodies

et al. 2022

View full text Add to dashboard Cite

The current influenza vaccines only confer protection against the circulating influenza subtypes, therefore universal vaccines are needed to prevent upcoming influenza outbreaks caused by emerging influenza subtypes. The extracellular domain of influenza A M2 protein (M2e) is highly conserved among different subtypes of influenza A viruses, and it is able to elicit protective immunity against the viruses. The influenza nucleoprotein (NP) was used to display the M2e in this study due to its promising T-cell response and adjuvanticity. The M2e gene was fused to the 5′-end of the NP gene and then cloned into pRSET B vector. The DNA sequencing analysis revealed six point mutations in the M2e-NP fusion gene, including one mutation in the M2e peptide and five mutations in the NP. The mutations were reverted using PCR site-directed mutagenesis. The recombinant plasmids (pRSET B-M2e-NP and pRSET B-mM2e-NP) were introduced into Escherichia coli (E. coli) BL21 (DE3) for protein expression. The mutated and non-mutated proteins were subsequently expressed and named mM2e-NP and M2e-NP, respectively. The expression of mM2e-NP and M2e-NP was not affected by the mutations. The binding of anti-M2e antibody to the purified native mM2e-NP and M2e-NP also remained active. However, when the anti-NP antibody was tested, the signal produced by mM2e-NP was very weak. The results implied that the amino acid changes in the NP had adversely impacted on the conformation of mM2e-NP and subsequently affected the antibody binding. In light of the remarkable antibody binding to the M2e-NP fusion protein, this study highly recommends the potential of M2e-NP as a universal influenza vaccine candidate.

show abstract

Pathogenesis and Pathological Changes of Avian Influenza in Human

Cited by 3 publications

References 10 publications

Alkaloids As Potential Antiviral : A Comprehensive Review

Alkaloids As Potential Antiviral : A Comprehensive Review

Alkaloids as potential antivirals. A comprehensive review

Expression of Influenza M2e-NP Recombinant Fusion Protein in Escherichia coli BL21 (DE3) and Its Binding to Antibodies

Contact Info

Product

Resources

About