Past, present and future of ICSI in livestock species

Briski, O.; Salamone, D.

doi:10.1016/j.anireprosci.2022.106925

Cited by 18 publications

(13 citation statements)

References 172 publications

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“…While IVF has been performed successfully in bovine, [ 30 ], ICSI bovine and porcine embryos fail to develop in vitro, and low blastocyst rates are obtained [ 31 ]. However, this can be improved by assisted activation [ 32 , 33 , 34 ]. Our work demonstrated that bovine oocytes activated by using a Zn chelator and, bypassing Ca oscillations [ 13 ], can develop up to the blastocyst stage, but the developmental rates and quality of these embryos are compromised.…”

Section: Discussionmentioning

confidence: 99%

Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation

Cabeza

Cámera

Briski

et al. 2022

Animals

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After sperm-oocyte fusion, intracytoplasmic rises of calcium (Ca) induce the release of zinc (Zn) out of the oocyte (Zn sparks). Both phenomena are known to play an essential role in the oocyte activation process. Our work aimed to explore different protocols for activating bovine and porcine oocytes using the novel zinc chelator 1,10-phenanthroline (PHEN) and to compare developmental rates and quality to bovine IVF and parthenogenetic ionomycin-induced embryos in both species. Different incubation conditions for the zinc chelator were tested, including its combination with ionomycin. Embryo quality was assessed by immunofluorescence of SOX2, SOX17, OCT4, and CDX2 and total cell number at the blastocyst stage. Even though blastocyst development was achieved using a zinc chelator in bovine, bypassing calcium oscillations, developmental rates, and blastocyst quality were compromised compared to embryos generated with sperm-induced or ionomycin calcium rise. On the contrary, zinc chelation is sufficient to trigger oocyte activation in porcine. Additionally, we determined the optimal exposure to PHEN for this species. Zinc chelation and artificial induction of calcium rise combined did not improve developmental competence. Our results contribute to understanding the role of zinc during oocyte activation and preimplantation embryo development across different mammalian species.

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Section: Discussionmentioning

confidence: 99%

Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation

Cabeza

Cámera

Briski

et al. 2022

Animals

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“…This inefficiency can be due to spermatozoa delayed or incomplete nucleus decondensation [21]. Even though IVF is widely used, the development of ICSI in bovine could be useful to decrease the generation interval, use of high valuable males and use semen samples with low sperm numbers, such as those obtained after sex-sorted [22]. Interestingly, in IVF it is necessary to use at least 10,000 spermatozoa per oocyte to have a good cleavage rate [23] suggesting that not all the spermatozoa are equally competent to fertilize the oocyte.…”

Section: Introductionmentioning

confidence: 99%

Bull spermatozoa selected by thermotaxis exhibit high DNA integrity, specific head morphometry, and improve ICSI outcome

Ruiz-Díaz¹,

Mazzarella²,

Navarrete-López³

et al. 2023

J Animal Sci Biotechnol

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Background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DNA fragmentation (SDF) in mice, humans, and stallions. This study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by ICSI. Methods We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TALP medium supplemented with 25 mmol/L of HEPES and 5 mmol/L of caffeine. In these conditions, sperm selection was achieved, obtaining a net thermotaxis of 3.6%. Subsequently, we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay, and we evaluated the size of the sperm head using Hemacolor® staining with Motic Images Plus 3 software. Additionally, migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured (IVM) oocytes by ICSI, a very inefficient procedure in cattle that is only successful when the oocyte is artificially activated. Results The results showed lower SDF (χ², P < 0.001, 13.3% reduction, n = 8) and lower head size parameters (length and width, P < 0.01; and perimeter and area, P < 0.001; n = 4) in those spermatozoa migrated in comparison to those not-migrated. The distribution of sperm subpopulations structure varied between groups, highlighting cluster 2, characterized by spermatozoa with small head size, and high ellipticity and elongated heads, as the most abundant in the thermotaxis migrated group. When performed ICSI (without oocyte artificial activation) with the thermotactic sperm, the blastocyst rate was 32.2% ± 9.3% in the group microinjected with the thermotactic spermatozoa vs. 8.3% ± 7.8% in the group of not-migrated sperm (χ², P < 0.05). Conclusion Our results showed that bull sperm selection by thermotaxis has a much higher DNA integrity, small and elongated head size parameters, and different sperm subpopulation structure than the not-selected spermatozoa. Additionally, we evidenced that thermotactic spermatozoa improve ICSI success rates.

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“…For some species with unsuccessful breeding programmes ( Lees & Wilcken 2009 ), cryopreserving (freezing cells with cryoprotectants enabling long-term viable cell and tissue storage), followed by cryobanking (indefinite storage of viable cells and tissue in liquid nitrogen at −196°C or ultra-low freezers) and assisted or advanced assisted reproductive technology (ART/aART) can save the genotypes that are being lost today ( Mitchell & Williams 2022 , for definitions please see Supplementary Table 1, see section on supplementary materials given at the end of this article). ART includes techniques that utilise oocytes and spermatozoa to generate offspring such as artificial insemination (AI), in vitro fertilisation (IVF) or intra cytoplasmic sperm injection (ICSI) ( Brown et al 2004 , Howard et al 2016 , Briski & Salamone 2022 ). These techniques are used for a number of taxa, but species-specific protocols need honing for many endangered species.…”

Section: Introductionmentioning

confidence: 99%

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

Bolton¹,

Mooney²,

Pettit

et al. 2022

Reproduction and Fertility

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Biodiversity: the variety of all living organisms on Earth is essential for human survival. However, anthropogenic activities are causing the sixth mass extinction, threatening even our own species. For many animals, dwindling numbers are becoming fragmented populations with low genetic diversity, threatening long-term species viability. With extinction rates 1,000-10,000 times greater than is natural, ex situ and in situ conservation programmes need additional support to save species. The indefinite storage of cryopreserved (-196oC) viable cells and tissues (cryobanking), followed by assisted or advanced assisted reproductive technology (ART: utilisation of oocytes and spermatozoa to generate offspring; aART: utilisation of somatic cell genetic material to generate offspring), may be the only hope for species long-term survival. As such, cryobanking should be considered a necessity for all future conservation strategies. Following cryopreservation, ART/aART can be used to reinstate lost genetics back into a population, resurrecting biodiversity. However, for this to be successful, species-specific protocol optimisation and increased knowledge of basic biology for many taxa is required. Current ART/aART is primarily focused on mammalian taxa, however, this needs to be extended to all, including to some of the most endangered: amphibians. Gamete, reproductive tissue and somatic cell cryobanking can fill the gap between losing genetic diversity today, and future technological developments. This review explores species prioritisation for cryobanking and the successes and challenges of cryopreservation and multiple ARTs/aARTs. We discuss the value of cryobanking before more species are lost and the potential of advanced reproductive technologies not only to halt, but reverse biodiversity loss.

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Past, present and future of ICSI in livestock species

Cited by 18 publications

References 172 publications

Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation

Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation

Bull spermatozoa selected by thermotaxis exhibit high DNA integrity, specific head morphometry, and improve ICSI outcome

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

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