Past, Present, and Future Industrial Biotechnology in China

Li, Zhenjiang; Ji, Xiao‐Jun; Kan, Suli; Qiao, Hongqun; Jiang, Min; Lu, Dingqiang; Wang, Jun; Huang, He; Jia, Honghua; Ouyuang, Pingkai; Ying, Hanjie

doi:10.1007/10_2010_76

Cited by 11 publications

(11 citation statements)

References 14 publications

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“…And the seed culture was prepared in a 250-ml Erlenmeyer flask containing 50 ml fresh seed medium inoculated with a full loop of K. oxytoca from fresh slant tube and cultivated at 37°C on a rotary shaker at 200 rpm for 24 h and subsequently inoculated into the bioreactor at 5% (v/v). The initial fermentation medium was consisted of (g/l): glucose, 80 (fed-batch fermentation) or 220 (batch fermentation); K 2 [22]. The glucose concentration in feeding medium was 800 g/l.…”

Section: Microorganism and Mediamentioning

confidence: 99%

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Nie

Huang

et al. 2010

Appl Biochem Biotechnol

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Substrate concentration in 2,3-butanediol (2,3-BD) fermentation could not be controlled well in traditional feeding strategies, such as constant, impulse, and exponential feeding strategies. In the present study, fermentative 2,3-BD production by Klebsiella oxytoca was investigated under different batch and fed-batch strategies. The glucose-feedback fed-batch strategy was proved to be not effective for economical 2,3-BD production for the inability of timely feeding, leading that the bacteria reused 2,3-BD as carbon source for cell growth. Based on the phenomena that the byproducing acids caused the pH declining and the requirement of maintaining the pH at a proper level for both cell growth and 2,3-BD accumulation, an improved strategy of pH-stat fed-batch culture with glucose and sodium hydrate fed at the same time was established. Thus, the residual glucose concentration could be controlled through the adjustment of pH automatically. At last, efficient 2,3-BD production was fulfilled under this fed-batch strategy, and the highest 2,3-BD concentration, productivity, and yield were 127.9 g/l, 1.78 g/(l•h), and 0.48 g/g (2,3-BD/glucose), respectively, compared to 98.5 g/l, 1.37 g/(l•h), and 0.43 g/g obtained in glucose-feedback fed-batch strategy. This feeding strategy was simple and easy to operate and could be feasible for industrial 2,3-BD production in the future.

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Section: Microorganism and Mediamentioning

confidence: 99%

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Nie

Huang

et al. 2010

Appl Biochem Biotechnol

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“…L-Phenylalanine is also used as a building block for the synthesis of aspartame, an artificial sweetener: poly-gamma-glutamic acid, which is used in the synthesis of biodegradable nanoparticles for pharmaceutical and biomedical applications. The worldwide production of L-phenylalanine was estimated to be more than 30 000 tons per year in 2014 (Li et al, 2010(Li et al, ). et al, 2014Maeda & Dudareva, 2012;Rodríguez et al, 2014;Zhang et al, 2013) (Fig.…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas putida as a platform for the synthesis of aromatic compounds

et al. 2016

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Aromatic compounds such as L-phenylalanine, 2-phenylethanol and trans-cinnamate are aromatic compounds of industrial interest. Current trends support replacement of chemical synthesis of these compounds by 'green' alternatives produced in microbial cell factories. The solvent-tolerant Pseudomonas putida DOT-T1E strain was genetically modified to produce up to 1 g l À1 of L-phenylalanine. In order to engineer this strain, we carried out the following stepwise process: (1) we selected random mutants that are resistant to toxic phenylalanine analogues; (2) we then deleted up to five genes belonging to phenylalanine metabolism pathways, which greatly diminished the internal metabolism of phenylalanine; and (3) in these mutants, we overexpressed the pheA fbr gene, which encodes a recombinant variant of PheA that is insensitive to feedback inhibition by phenylalanine. Furthermore, by introducing new genes, we were able to further extend the diversity of compounds produced. Introduction of histidinol phosphate transferase (PP_0967), phenylpyruvate decarboxylase (kdc) and an alcohol dehydrogenase (adh) enabled the strain to produce up to 180 mg l À1 2-phenylethanol. When phenylalanine ammonia lyase (pal) was introduced, the resulting strain produced up to 200 mg l À1 of trans-cinnamate. These results demonstrate that P. putida can serve as a promising microbial cell factory for the production of L-phenylalanine and related compounds.

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“…Thus, validamycin A has been an important commodity in both the pharmaceutical and agricultural industries. In China alone, about 40,000 tons of validamycin are produced annually (8).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and metabolic engineering of pseudo-oligosaccharides

Alanzi

Demessie

Mahmud

2018

Emerging Topics in Life Sciences

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Pseudo-oligosaccharides are microbial-derived secondary metabolites whose chemical structures contain pseudosugars (glycomimetics). Due to their high resemblance to the molecules of life (carbohydrates), most pseudo-oligosaccharides show significant biological activities. Some of them have been used as drugs to treat human and plant diseases. Because of their significant economic value, efforts have been put into understanding their biosynthesis, optimizing their fermentation conditions, and engineering their metabolic pathways to obtain better production yields. A number of unusual enzymes participating in diverse biosynthetic pathways to pseudo-oligosaccharides have been reported. Various methods and conditions to improve the production yields of the target compounds and eliminate byproducts have also been developed. This review article describes recent studies on the biosynthesis, fermentation optimization, and metabolic engineering of high-value pseudo-oligosaccharides.

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Past, Present, and Future Industrial Biotechnology in China

Cited by 11 publications

References 14 publications

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Pseudomonas putida as a platform for the synthesis of aromatic compounds

Biosynthesis and metabolic engineering of pseudo-oligosaccharides

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