Passive evasion of encapsulation in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae), a polyembryonic parasitoid of Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae)

Hu, Jian; Zhu, Xiaopeng; Fu, Wenjun

doi:10.1016/s0022-1910(03)00021-0

Cited by 66 publications

(62 citation statements)

References 27 publications

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“…This was consistent with the previous report in which granulocytes and plasmatocytes were described as making up more than 50% of the circulating haemocytes in lepidopteran larval stages (Strand & Pech, 1995;Lavine & Strand, 2002). Hu et al (2003) even demonstrated that granular haemocytes and plasmatocytes comprised > 90% of the total haemocyte population. These two types of haemocytes are also the only haemocyte types capable of adhering to foreign surfaces and eliminating the invaders (Elrod-Erickson et al, 2000).…”

Section: Discussionsupporting

confidence: 93%

“…Hu et al (2003) also identifi ed these fi ve haemocyte types in O. furnacalis larvae under phase contrast microscopy and transmission electron microscopy. Furthermore, granulocytes and plasmatocytes were more common than the other three types of haemocytes, although the exact proportion of granulocytes and plasmatocytes was not recorded due to the rare observation of prohaemocytes and spherulocytes.…”

Section: Discussionmentioning

confidence: 97%

“…Then 3 μl of (Blattodea: Isoptera: Rhinotermitidae) or Phytomonas serpens-induced Large milkweed bugs, Oncopeltus fasciatus (Dallas) (Hemiptera: Lygaeidae) increased signifi cantly, while the pool of circulating haemocytes became depleted in Bacillus thuringiensis-infected Greater wax moths, Galleria mellonella (L.) (Lepidoptera: Pyralidae) (Chouvenc et al, 2009; Alves e Silva et al, 2013). In Macrocentrus cingulum-parasitized Asian corn borer moth larvae, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae), the total number of haemocytes remained unchanged for 4 days, but decreased signifi cantly 5 days post parasitization (Hu et al, 2003).…”

Section: Effect Of Plasma On Phagocytosis and Nodulation In O Furnacmentioning

confidence: 99%

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Cellular immune response of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae), to infection by the entomopathogenic fungus, Beauveria bassiana

Shen¹,

Li²,

Cheng³

et al. 2016

Eur. J. Entomol.

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Abstract. The term cellular immune response refers to haemocyte-mediated responses, including phagocytosis, nodulation, and encapsulation. In the present study, we identifi ed fi ve types of circulating haemocytes in larvae of the haemolymph of the Asian corn borer, Ostrinia furnacalis (Guenée), including granulocytes, oenocytoids, plasmatocytes, prohaemocytes, and spherulocytes. The relative number of total free haemocytes per larva decreased signifi cantly 0.5, 24, and 36 h after the injection of Beauveria bassiana conidia. Upon conidia challenge, both phagocytosis and nodulation were observed in the collected haemolymph from O. furnacalis larvae. In addition, plasma was found to be necessary for both phagocytosis and nodulation. Therefore, we here confi rm that phagocytosis and nodulation are involved in O. funacalis larvae during their fi ght against infection by B. bassiana, and further, that the cellular immune response of O. furnacalis helps eliminate the invading organisms despite the fact that not all the fungal conidia are killed.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Section: Effect Of Plasma On Phagocytosis and Nodulation In O Furnacmentioning

confidence: 99%

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Cellular immune response of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae), to infection by the entomopathogenic fungus, Beauveria bassiana

Shen¹,

Li²,

Cheng³

et al. 2016

Eur. J. Entomol.

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“…Although several potential horizontal transfer routes can exist in all Maculinea species, e.g. transfer by Myrmica host ants being in regular physical contact with caterpillars (ant workers feed them by regurgitation or caterpillars feed on the ant brood) or transfer by parasitoids (in certain cases the host"s immune system may neutralise the parasitoid larva, see Davies and Vinson 1986;Hu et al 2003), the lifecycle of M. teleius and M. nausithous is closely related to each other in many respects. Females in both species lay their eggs on Sanguisorba officinalis.…”

Section: Discussionmentioning

confidence: 99%

Controversial patterns of Wolbachia infestation in the social parasitic Maculinea butterflies (Lepidoptera: Lycaenidae)

et al. 2015

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Wolbachia is a common group of intracellular bacteria found in arthropods and filarial nematodes. Since the past decade they have attracted considerable interest owing to their various effects on hosts, which range from reproductive manipulation to mutualism. Moreover, they can influence the mitochondrial DNA pattern which do not reflect the real evolutionary history of the target species and may be incongruent with nuclear data.Previously Wolbachia-manipulated mtDNA patterns, namely mito-nuclear discordance and deep mitochondrial splits associated with specific Wolbachia infections have been also discovered in the genus Maculinea. Here we present a comprehensive study on Wolbachia infestation and the genetic diversity of all Maculinea species in the Carpathian Basin. The prevalence and the pattern of the infestation highly differ among Maculinea species. M. alcon and M. arion are infected in 100%, each of these species with a single strain, but the infection level of M. nausithous and M. teleius is much lower, additionally, they are infected with multiple strains. The genetic diversity of Maculinea species proved to be generally low, only M. nausithous showed geographic pattern based on mitochondrial sequences and allozymes. In contrast with the previous studies, we could not detect mitonuclear discordance or find evidence for Wolbachia-induced selective sweep. Based on our results, we cannot hold only Wolbachia responsible for the restricted genetic diversity of Maculinea in the Carpathian Basin.Probably several factors shape together the level and pattern of genetic variability in Maculinea butterflies.

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“…Parasitoid effectiveness depends on their searching ability to locate the host (Nordlund et al, 1988). Macrocentrus cingulum Brischke (Hymenoptera: Braconidae) is a polyembryonic endoparasitoid of the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae) and the European corn borer, O. nubilalis (Hübner) (Edwards & Hopper, 1999;Hu et al, 2003). The native range of M. cingulum is distributed across Europe and throughout Asia, including Japan, Korea and China (Watanabe, 1967).…”

Section: Rt-pcr For Expression Analysismentioning

confidence: 99%

Identification and expression pattern analysis of chemosensory receptor genes in the Macrocentrus cingulum (Hymenoptera: Braconidae) antennae

Ahmed¹,

Zhang²,

Wang³

et al. 2016

Eur. J. Entomol.

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Abstract.Macrocentrus cingulum is an important polyembryonic endoparasitic wasp that attacks larvae of the Asian corn borer, Ostrinia furnacalis (Guenée) and the European corn borer, O. nubilalis (Hübner). Parasitoids use antennae as the main sensory organ to recognize herbivore-induced plant volatiles as host searching cues. The antennal olfaction proteins, odorant receptors (ORs) and ionotropic receptors (IRs) are involved in olfactory signal transduction pathway as a sensory neuron response. In the present study, we constructed a cDNA library from the male and female antennae for identifying the olfaction-related genes in M. cingulum. For that, we sequenced 3160 unique gene sequences and annotated them with gene ontology (GO), cluster of orthologous groups of proteins (COG), and KEGG ontology (KO). Through the homology search, we identifi ed 9 odorant receptors (ORs), 3 ionotropic receptors (IRs) and 1 odorant binding protein (OBP) genes from the cDNA library sequences. Additionally, the expression patterns of these ORs and IRs in different tissues (antennae, heads, thoraxes, abdomens, and legs) were demonstrated by RT-PCR. The qualitative gene expression analyses showed that most of the OR genes were more highly expressed in female than male antennae; whereas IRs, unlike ORs, were more expressed in various male than females tissues. We are the fi rst to report ORs and IRs in M. cingulum, which should help in deciphering the molecular basis of olfaction system in this wasp.

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Passive evasion of encapsulation in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae), a polyembryonic parasitoid of Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae)

Cited by 66 publications

References 27 publications

Cellular immune response of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae), to infection by the entomopathogenic fungus, Beauveria bassiana

Cellular immune response of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae), to infection by the entomopathogenic fungus, Beauveria bassiana

Controversial patterns of Wolbachia infestation in the social parasitic Maculinea butterflies (Lepidoptera: Lycaenidae)

Identification and expression pattern analysis of chemosensory receptor genes in the Macrocentrus cingulum (Hymenoptera: Braconidae) antennae

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