Passage of Salmonella enteritidis and Salmonella thompson through chick ileocecal mucosa

Abstract

The passage of Salmonella enteritidis and S. thompson across the cecal mucosa has been visualized in an electron microscope study with the freshly hatched chick as a model. The uptake of salmonellae by macrophages took place in the cecal lumen; the macrophages became abnormal and often ruptured to release organisms back into the lumen. The entry of bacteria into the epithelial cells was associated with a series of pathological changes, beginning with the appearance of active Golgi apparatus and the production of a variety of lysosomal vesicles. Salmonellae became sequestered within lysosomes but were unaffected by the presence of hydrolytic enzyme. Epithelial cell death was related to particularly large numbers of bacteria. Fragments of invaded epithelial cells, especially those undergoing cell death, contributed to the cytoplasmic debris and released further salmonellae into the lumen. Bacteria were never observed in large numbers below the basement membrane, and there was no significant pathology in the lamina propria tissue. Wandering cells, identified as macrophages and containing the bacteria, were observed spanning the epithelial and lamina propria regions through breaks in the basement membrane. It is suggested that the passage of bacteria from the epithelium to the lamina propria is primarily the result of capture and transport within host macrophages.

Effects of Orally Administered Bdellovibrio bacteriovorus on the Well-Being and Salmonella Colonization of Young Chicks

“…That many of the Salmonella-treated birds which did not receive predatory Bdellovibrio had pale ceca with abnormal dense white mucoid gut material within suggested that the untreated Salmonella had caused the inflammation of the ceca and recruitment of heterophils to that area of the gut, as others have reported (1,2,16,36,41,42). It was marked how treatment with predatory Bdellovibrio, but not the ⌬pilA strain, had ameliorated this effect (Table 1 and Fig.…”

Effects of Orally Administered Bdellovibrio bacteriovorus on the Well-Being and Salmonella Colonization of Young Chicks

“…That many of the Salmonella-treated birds which did not receive predatory Bdellovibrio had pale ceca with abnormal dense white mucoid gut material within suggested that the untreated Salmonella had caused the inflammation of the ceca and recruitment of heterophils to that area of the gut, as others have reported (1,2,16,36,41,42). It was marked how treatment with predatory Bdellovibrio, but not the ⌬pilA strain, had ameliorated this effect (Table 1 and Fig.…”

Mutation of invH , but Not stn , Reduces Salmonella -Induced Enteritis in Cattle

“…Bacteria have frequently been observed within lamina propria macrophages of intestines infected with salmonellas (28, 35), and so the effect of the invH mutation on the interaction of S. typhimurium with bovine alveolar macrophages was assessed. Intestinal macrophages were the cells of choice to use for this experiment, but it was not possible to recover either a pure cell population or sufficient numbers for these experiments (data not shown).…”

In vitro attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis phage type 8

“…Invasion of intestinal walls by S. enteritidis leads to systemic spread of the organism (6,14,15,33,36). Previous studies indicated that following invasion of intestinal epithelial cells, S. enteritidis were found in macrophages in the lamina propria (32). The colonization of preovulatory follicles by blood-borne S. enteritidis could possibly involve interactions with the cellular components in the ovarian follicular wall (35).…”

Effects of Orally Administered Bdellovibrio bacteriovorus on the Well-Being and Salmonella Colonization of Young Chicks

“…That many of the Salmonella-treated birds which did not receive predatory Bdellovibrio had pale ceca with abnormal dense white mucoid gut material within suggested that the untreated Salmonella had caused the inflammation of the ceca and recruitment of heterophils to that area of the gut, as others have reported (1,2,16,36,41,42). It was marked how treatment with predatory Bdellovibrio, but not the ⌬pilA strain, had ameliorated this effect (Table 1 and Fig.…”

Mutation of invH , but Not stn , Reduces Salmonella -Induced Enteritis in Cattle

“…Bacteria have frequently been observed within lamina propria macrophages of intestines infected with salmonellas (28, 35), and so the effect of the invH mutation on the interaction of S. typhimurium with bovine alveolar macrophages was assessed. Intestinal macrophages were the cells of choice to use for this experiment, but it was not possible to recover either a pure cell population or sufficient numbers for these experiments (data not shown).…”

In vitro attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis phage type 8

“…Invasion of intestinal walls by S. enteritidis leads to systemic spread of the organism (6,14,15,33,36). Previous studies indicated that following invasion of intestinal epithelial cells, S. enteritidis were found in macrophages in the lamina propria (32). The colonization of preovulatory follicles by blood-borne S. enteritidis could possibly involve interactions with the cellular components in the ovarian follicular wall (35).…”

Effects of Orally Administered Bdellovibrio bacteriovorus on the Well-Being and Salmonella Colonization of Young Chicks

“…That many of the Salmonella-treated birds which did not receive predatory Bdellovibrio had pale ceca with abnormal dense white mucoid gut material within suggested that the untreated Salmonella had caused the inflammation of the ceca and recruitment of heterophils to that area of the gut, as others have reported (1,2,16,36,41,42). It was marked how treatment with predatory Bdellovibrio, but not the ⌬pilA strain, had ameliorated this effect (Table 1 and Fig.…”

Mutation of invH , but Not stn , Reduces Salmonella -Induced Enteritis in Cattle

“…Bacteria have frequently been observed within lamina propria macrophages of intestines infected with salmonellas (28, 35), and so the effect of the invH mutation on the interaction of S. typhimurium with bovine alveolar macrophages was assessed. Intestinal macrophages were the cells of choice to use for this experiment, but it was not possible to recover either a pure cell population or sufficient numbers for these experiments (data not shown).…”

In vitro attachment and invasion of chicken ovarian granulosa cells by Salmonella enteritidis phage type 8

“…Invasion of intestinal walls by S. enteritidis leads to systemic spread of the organism (6,14,15,33,36). Previous studies indicated that following invasion of intestinal epithelial cells, S. enteritidis were found in macrophages in the lamina propria (32). The colonization of preovulatory follicles by blood-borne S. enteritidis could possibly involve interactions with the cellular components in the ovarian follicular wall (35).…”